LOCUS       sequence-68117-e        1134 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  wt esaR gene under control of Plac in pACYC184.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1134)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 1134)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR
FEATURES             Location/Qualifiers
     source          1..1134
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    108..129
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(144..149,150..167,168..174)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    182..198
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     187..209
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     RBS             216..227
                     /note="strong bacterial ribosome binding site (Elowitz and 
                     Leibler, 2000)"
     terminator      1008..1079
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     terminator      1095..1122
                     /label=T7Te terminator
                     /note="phage T7 early transcription terminator"
ORIGIN
        1 tctagaccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
       61 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
      121 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
      181 attgtgagcg gataacaatt tcacacagga aacataaaga ggagaaaggt acccatgatg
      241 ttctctttct tccttgaaaa ccaaacaata acggatacgc ttcagactta catacagaga
      301 aagttatctc cgctgggtag tccggattac gcttacactg ttgtgagcaa aaaaaatcct
      361 tcaaatgttc tgattatttc cagttatcct gacgaatgga ttaggttata ccgcgctaac
      421 aactttcagc tgaccgatcc ggttattctc acggccttta aacgcacctc gccgtttgcc
      481 tgggatgaga atattacgct gatgtccgac ctgcggttca ccaaaatttt ctctttatcc
      541 aagcaataca acatcgttaa cggctttacc tatgtcctgc atgaccacat gaacaacctt
      601 gctctgttgt ccgtgatcat taaaggcaac gatcagactg cgctggagca acgccttgct
      661 gccgaacagg gcacgatgca gatgctgctg attgatttta acgagcagat gtaccgactg
      721 gcaggcaccg aaggtgaacg agcaccggcg ttaaatcaga gcgcggacaa aacgatattt
      781 tcctcgcgtg aaaatgaggt gttgtactgg gcgagtatgg gcaaaaccta tgctgagatt
      841 gccgctatta cgggcatttc tgtgagtacc gtgaagtttc acatcaagaa tgtggtcgtg
      901 aaactgggcg tcagtaacgc ccgacaggct atcagactgg gtgtagaact ggatcttatc
      961 agaccggcag cgtcagcagc aaggtagtga tagggatccc caggcatcaa ataaaacgaa
     1021 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     1081 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttatagt cgac
//
