LOCUS       sequence-68127-e         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pAC-14-LEXA-QF
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..29
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(39..290,291..644)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 aaatgccacc caagaagaag cgaaaagtag aagatccaat gaaggctctc acggcccgac
       61 aacaggaagt ttttgatttg atacgggatc atatatccca aacgggtatg cctccgaccc
      121 gcgcagagat agcacagcga ctgggctttc gatcgcctaa cgccgcggag gagcacttga
      181 aggcactggc ccgcaagggt gtcattgaaa tcgtgtccgg tgcgagccgc ggaatccggc
      241 tgttgcagga ggaggaagag ggcctgccac tggtgggacg cgtggccgct ggcgagccgc
      301 tgctggccca gcaacacata gagggacact atcaggtgga cccctccttg tttaagccaa
      361 atgctgattt cctgttgcgg gtgtcgggaa tgtccatgaa ggacatcggt attatggatg
      421 gtgacctcct cgccgtccat aagacacagg atgtcaggaa cggccaggta gtcgttgcca
      481 ggatagacga tgaggtcact gtgaaacgtc tcaagaagca aggcaataag gtcgagctgc
      541 tgccggagaa tagcgagttc aagccgatcg tggtggatct gcgacagcag tcctttacta
      601 tcgagggctt ggccgtgggt gtgatccgca acggagattg gctgggacgt cagttggagc
      661 tccctcctac cgccacggct acggcctcga taatgccgca cgtgatggag cagcctctca
      721 gtaccagcat taaccccgtc aacgaccgct tcaacggtat tcccaacccc actccgtata
      781 actccgatgc agctctcgat gctatcactc agaccaacga ttatgg
//
