LOCUS       sequence-68130-e         920 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 920)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 920)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pAC-15-LexABD-G4MD-QFAD
FEATURES             Location/Qualifiers
     source          1..920
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             17..37
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(47..298,299..652)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 gaattccaaa atgccaccca agaagaagcg aaaagtagaa gatccaatga aggctctcac
       61 ggcccgacaa caggaagttt ttgatttgat acgggatcat atatcccaaa cgggtatgcc
      121 tccgacccgc gcagagatag cacagcgact gggctttcga tcgcctaacg ccgcggagga
      181 gcacttgaag gcactggccc gcaagggtgt cattgaaatc gtgtccggtg cgagccgcgg
      241 aatccggctg ttgcaggagg aggaagaggg cctgccactg gtgggacgcg tggccgctgg
      301 cgagccgctg ctggcccagc aacacataga gggacactat caggtggacc cctccttgtt
      361 taagccaaat gctgatttcc tgttgcgggt gtcgggaatg tccatgaagg acatcggtat
      421 tatggatggt gacctcctcg ccgtccataa gacacaggat gtcaggaacg gccaggtagt
      481 cgttgccagg atagacgatg aggtcactgt gaaacgtctc aagaagcaag gcaataaggt
      541 cgagctgctg ccggagaata gcgagttcaa gccgatcgtg gtggatctgc gacagcagtc
      601 ctttactatc gagggcttgg ccgtgggtgt gatccgcaac ggagattggc tgggatccat
      661 cgacagtgcg gcccaccacg acaacagcac cattccactg gacttcatgc ctcgtgacgc
      721 actgcatgga tttgactgga gcgaggagga tgatatgagc gacggactcc cattcctgaa
      781 gaccgacccc aataacaatg gtttctttgg cgacggcagc ctgctgtgta tcctgcgaag
      841 tattggtttt aagccggaaa attacacgaa cagtaatgtc aatcgtcttc ccacaatgat
      901 caccgaccgt tacacgctgg
//
