LOCUS       sequence-68133-e         871 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 871)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 871)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-2-G4BDMD-QFAD-hsp70
FEATURES             Location/Qualifiers
     source          1..871
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             24..464
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="DNA binding domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 DNA binding domain
                     /translation="MKLLSSIEQACDICRLKKLKCSKEKPKCAKCLKNNWECRYSPKTK
                     RSPLTRAHLTEVESRLERLEQLFLLIFPREDLDMILKMDSLQDIKALLTGLFVQDNVNK
                     DAVTDRLASVETDMPLTLRQHRISATSSSEESSNKGQRQLTVS"
ORIGIN
        1 atcatcgaca gccgaattcc aacatgaagc tactgtcttc tatcgaacaa gcatgcgata
       61 tttgccgact taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga
      121 agaacaactg ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac
      181 atctgacaga agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc
      241 ctcgagaaga ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt
      301 taacaggatt atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt
      361 cagtggagac tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat
      421 cggaagagag tagtaacaaa ggtcaaagac agttgactgt atcgattgac tcggcagctc
      481 atcatgataa ctccacaatt ccgttggatt ttatgcccag ggatgctctt catggatttg
      541 attggtctga agaggatgac atgtcggatg gcttgccctt cctgaaaacg gaccccaaca
      601 ataatgggtt ctttggcgac ggttctctct tatgtattct tcgatctatt ggctttaaac
      661 cggaaaatta cacgaactct aacgttaaca ggctcccgac catgattacg gatagataca
      721 cgttggcttc tagatccaca acatcccgtt tacttcaaag ttatctcaat aattttcacc
      781 cctactgccc tatcgtgcac tcaccgacgc taatgatgtt gtataataac cagattgaaa
      841 tcgcgtcgaa ggatcaatgg caaatccttt t
//
