LOCUS       sequence-68144-e         769 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 769)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 769)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyNT
FEATURES             Location/Qualifiers
     source          1..769
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(115..161)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(258..278)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(291..308)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 ggatctctcc ggcttcaccg atccggagga actggcgcag ttcctctggg ggccgccgga
       61 caccgccccc ggcgcctgac gccccctcta gctgatcacc gcggccatga tggcataaaa
      121 cgaaaggccc agtctttcga ctgagccttt cgttttattt gatgcctggc agtcgatcgt
      181 acgctagtta actacgtcga catcgataag cttgctagcg atatcgaatt cggatccggt
      241 accttatcgt ccatggcgcc ctggaagtac aggttctcgc cggccgacgg gtggtggtgg
      301 tggtggtgct tcatggtgga ctccctttct cttatcgggt ggtggccgcg agcgcggccg
      361 gtggagggtt ggtgactgac ggcgctgcga gacggcgccc gatggggtat gcgagaacaa
      421 ctgcggcaaa accgatcacg gcgatgaccg cggcggggag cgggccggtt tcccagttgc
      481 ccgacacgat caggaaggcc ggcacggcgg cggtgatcac gccttcggcc accgcgacga
      541 acccgacggc gggccccagt gcgtcgtgcc ccaggccgag cagcaggaac agcatgaacc
      601 acagcactgc ccacagcagc cagatcaccc cgaacgccgg gtcggcctcg gcggtgaacg
      661 cgtgccacga gtagccgagt gcggcgatcg cgacgaacag cgagaaccat ccgagacctt
      721 ctccgtccca gtcggtcacg ttgttgatgg ccacccacag gtaggtgaa
//
