LOCUS       sequence-68148-e         820 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 820)
  AUTHORS   Trempe JF, Sauve V, Grenier K, Seirafi M, Tang MY, Menade M, 
            Al-Abdul-Wahid S, Krett J, Wong K, Kozlov G, Nagar B, Fon EA, 
            Gehring K
  TITLE     Structure of Parkin Reveals Mechanisms for Ubiquitin Ligase 
            Activation.
  JOURNAL   Science. 2013 May 9.
  PUBMED    23661642
REFERENCE   2  (bases 1 to 820)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2013 May 9."
COMMENT     Sequence Label: pGEX-parkin 219-465
FEATURES             Location/Qualifiers
     source          1..820
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(717..740)
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
     primer_bind     complement(767..789)
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
ORIGIN
        1 ttccattcgc aaccgcagtt ccagcaccat tccagtttgc actgcggctg cgggcatttc
       61 atgtgcatgc aaccaccgtt tttttcgatc ggaacgttgc aacgcgggca cggtttggtg
      121 gtttttttga tggtttcttt agacgcttct tcccaacgcg cctgttccgc cgcacgctgg
      181 tcaacacggt acgcctgaga ggtcgcacca gacgcttcga acatagagtc gcattcacct
      241 tcgtggtacg cttctttgca gtcacggcag aaaacgaaac cgcaacccag accgttacca
      301 ccttcgcagg taactttacg ctgaccctgt tccggcagca gacccgcacc gcaacccgga
      361 cgcgggcaca gaacaccacc catctgcaga acgcattctt ccgcaccgta ctgctggtaa
      421 cggttgtact gttcttcacc caggatacgg aagtggtgca gttctttgat cagagagttc
      481 gggcaacccg caacgcacgg cagagagtaa cccagctgcg cgtcgtgaac gaactgacgg
      541 tcgttcagac gggtaacgca gtacaggtgg aagcagtcca ggcagataac gtgacggtgg
      601 ttgcactgga aaaccagaac cgggttacga acgtcggtgc acgcgatgca cgggatagaa
      661 cgagagttgt tggtgatcag gttcagcgca acagaggtgt ctttgtcgga tcccaggggc
      721 ccctggaaca gaacttccag atccgatttt ggaggatggt cgccaccacc aaacgtggct
      781 tgccagccct gcaaaggcca tgctatatac ttgctggaat
//
