LOCUS       sequence-68149-e         759 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 759)
  AUTHORS   Beckham KSH, Staack S, Wilmanns M, Parret AHA
  TITLE     The pMy vector series: A versatile cloning platform for the 
            recombinant production of mycobacterial proteins in Mycobacterium 
            smegmatis.
  JOURNAL   Protein Sci. 2020 Oct 2. doi: 10.1002/pro.3962.
  PUBMED    33006405
REFERENCE   2  (bases 1 to 759)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Protein 
            Sci. 2020 Oct 2. doi: 10.1002/pro.3962."
COMMENT     Sequence Label: pMyC
FEATURES             Location/Qualifiers
     source          1..759
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     terminator      complement(114..160)
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
     CDS             complement(219..236)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 gatctctccg gcttcaccga tccggaggaa ctggcgcagt tcctctgggg gccgccggac
       61 accgcccccg gcgcctgacg ccccctctag ctgatcaccg cggccatgat ggcataaaac
      121 gaaaggccca gtctttcgac tgagcctttc gttttatttg atgcctggca gtcgatcgta
      181 cgctagttaa ctacgtcgac atcgataagc tgttatcagt ggtggtggtg gtggtgcgaa
      241 gcttggctag cgatatcgaa ttcggatccg gtaccggccg agcccatggt ggactccctt
      301 tctcttatcg ggtggtggcc gcgagcgcgg ccggtggagg gttggtgact gacggcgctg
      361 cgagacggcg cccgatgggg tatgcgagaa caactgcggc aaaaccgatc acggcgatga
      421 ccgcggcggg gagcgggccg gtttcccagt tgcccgacac gatcaggaag gccggcacgg
      481 cggcggtgat cacgccttcg gccaccgcga cgaacccgac ggcgggcccc agtgcgtcgt
      541 gccccaggcc gagcagcagg aacagcatga accacagcac tgcccacagc agccagatca
      601 ccccgaacgc cgggtcggcc tcggcggtga acgcgtgcca cgagtagccg agtgcggcga
      661 tcgcgacgaa cagcgagaac catccgagac cttctccgtc ccagtcggtc acgttgttga
      721 tggccaccca caggtaggtg aagccgaaca ggtagagcc
//
