LOCUS       sequence-68158-e         772 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  pAC-EsaR with a272g and c659t mutations in esaR.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 772)
  AUTHORS   Shong J, Huang YM, Bystroff C, Collins CH
  TITLE     Directed evolution of the quorum-sensing regulator EsaR for 
            increased signal sensitivity.
  JOURNAL   ACS Chem Biol. 2013 Apr 19;8(4):789-95. doi: 10.1021/cb3006402. Epub
            2013 Feb 6.
  PUBMED    23363022
REFERENCE   2  (bases 1 to 772)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb3006402";
            journalName: "ACS Chem Biol"; date: "2013-04-19- 19"; volume: "8"; 
            issue: "4"; pages: "789-95"
COMMENT     Sequence Label: pAC-EsaR-D91G/V220A
FEATURES             Location/Qualifiers
     source          1..772
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 ggtacccatg atgttctctt tcttccttga aaaccaaaca ataacggata cgcttcagac
       61 ttacatacag agaaagttat ctccgctggg tagtccggat tacgcttaca ctgttgtgag
      121 caaaaaaaat ccttcaaatg ttctgattat ttccagttat cctgacgaat ggattaggtt
      181 ataccgcgct aacaactttc agctgaccga tccggttatt ctcacggcct ttaaacgcac
      241 ctcgccgttt gcctgggatg agaatattac gctgatgtcc ggcctgcggt tcaccaaaat
      301 tttctcttta tccaagcaat acaacatcgt taacggcttt acctatgtcc tgcatgacca
      361 catgaacaac cttgctctgt tgtccgtgat cattaaaggc aacgatcaga ctgcgctgga
      421 gcaacgcctt gctgccgaac agggcacgat gcagatgctg ctgattgatt ttaacgagca
      481 gatgtaccga ctggcaggca ccgaaggtga acgagcaccg gcgttaaatc agagcgcgga
      541 caaaacgata ttttcctcgc gtgaaaatga ggtgttgtac tgggcgagta tgggcaaaac
      601 ctatgctgag attgccgcta ttacgggcat ttctgtgagt accgtgaagt ttcacatcaa
      661 gaatgtggcc gtgaaactgg gcgtcagtaa cgcccgacag gctatcagac tgggtgtaga
      721 actggatctt atcagaccgg cagcgtcagc agcaaggtag tgatagggat cc
//
