LOCUS       sequence-6816-ex        9706 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9706)
  AUTHORS   Cowen LE, Lindquist S
  TITLE     Hsp90 potentiates the rapid evolution of new traits: drug resistance
            in diverse fungi.
  JOURNAL   Science. 2005 Sep 30. 309(5744):2185-9.
  PUBMED    16195452
REFERENCE   2  (bases 1 to 9706)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Science. 
            2005 Sep 30. 309(5744):2185-9."
COMMENT     Sequence Label: 7860 pRS305-pUG72-HSC82in
FEATURES             Location/Qualifiers
     source          1..9706
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(663..1757)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1770..2174)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2474..2929)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2611..2632)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2823..2842
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3059..3081
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3073..3090
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3074..3090
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3097..3116
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3097..3115
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    complement(3172..3205)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     protein_bind    complement(7324..7357)
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     primer_bind     complement(7400..7416)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7401..7417)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     promoter        complement(7446..7464)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(7485..7501)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(7485..7501)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(7498..7520)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    7509..7525
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(7533..7539,7540..7557,7558..7563))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(7716..7733)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(7887..8475)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(join(8646..9437,9438..9506))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFFHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9269..9288
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(9507..9611)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9679..9697
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgagggtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact atagggcgaa
     3121 ttggagctcc accgcggccg cataggccac tagtggatct gatatcacct aataacttcg
     3181 tatagcatac attatacgaa gttatattaa gggttctcga gagctcccca ttacgcattt
     3241 gattataatt tgcttcttag gcaaaattaa tatttacgtt cttttatatt ctttcttttt
     3301 gtattcatag aacagcagcc attaccaata gaaagttaaa atagccgccg atgcatttta
     3361 ttacccgcct ttctgttttc tgggcacttt tctttctaga aggtgaaaga acaatttttc
     3421 tcgttttctc gaacttccac caagcgttgg gtaatgaggg aggagatttg tataaaaaga
     3481 gtggcatgtg aactgcctac cgtaagtgac atgaacacat gcattatatt ttttgtgata
     3541 tattctttct cttgttttct ttttcttgaa acgctacaga accaatagaa aaatagaatc
     3601 attctgaaat atggctggtg aaacttttga atttcaagct gaaatcactc agttgatgag
     3661 tttgatcatc aacactgtct attctaacaa ggaaattttc ttgagagaac tgatctctaa
     3721 cgcctccgat gctttagaca aaattagata ccaagctttg tctgatccaa agcaattgga
     3781 aaccgaacca gatttgttca ttagaatcac cccaaaacca gaagaaaaag ttttggaaat
     3841 cagagattct ggtattggta tgaccaaggc tgaattgatt aacaatttgg gtaccattgc
     3901 taagtctggt actaaagctt tcatggaagc tctatctgct ggtgccgatg tatccatgat
     3961 tggtcaattc ggtgttggtt tttactcttt attcttagtc gccgacagag ttcaagttat
     4021 ttccaagaac aatgaggacg aacaatatat ttgggaatct aatgccggtg gttctttcac
     4081 cgttactttg gacgaagtta acgaaagaat tggtagaggt accgtcttga gattattctt
     4141 gaaagatgac caattggagt acttggaaga aaagagaatt aaagaagtca tcaagagaca
     4201 ttctgaattc gttgcttacc ctatccaact tctagtcacc aaggaagtcg aaaaggaagt
     4261 tccaattcca gaagaagaaa agaaagacga ggaaaagaag gatgaagatg acaagaaacc
     4321 aaaattggaa gaagtcgatg aagaagaaga agaaaagaag ccaaaaacca aaaaagttaa
     4381 agaagaggtt caagaattag aagagttgaa caagactaag ccattatgga ctagaaaccc
     4441 atctgatatc actcaagagg aatacaatgc tttctataag tctatttcta acgactggga
     4501 agacccattg tacgttaagc atttctctgt tgaaggtcaa ttggaattta gagctatctt
     4561 gttcattcca aagagagcac cattcgactt atttgagagt aagaagaaga agaacaatat
     4621 caagttgtac gttcgtcgtg tcttcatcac tgatgaagct gaagacttga ttccagagtg
     4681 gttatctttc gtcaagggtg ttgttgactc tgaagattta ccattgaatt tgtccagaga
     4741 aatgttacaa caaaataaga ttatgaaggt tattagaaag aatattgtca agaaattgat
     4801 tgaagccttc aacgaaatcg ctgaagactc cgagcaattt gacaaatttt actctgcctt
     4861 cgctaagaac attaagctgg gtgtacatga ggacactcaa aacagagctg ctttagctaa
     4921 gttgctacgt tacaattcta ctaaatctgt cgatgaattg acttccttga ctgattacgt
     4981 tactagaatg ccagaacacc aaaagaacat ctattacatc accggtgaat ctctaaaggc
     5041 agtcgaaaag tctccattct tggacgcctt gaaggctaag aactttgaag ttttgttctt
     5101 gaccgaccca attgatgaat acgctttcac tcaattgaag gaattcgagg gtaaaacttt
     5161 ggttgacatt actaaagatt tcgaattgga agaaacagac gaagaaaaag ctgaaagaga
     5221 gaaggagatc aaagaatacg aaccattgac caaggccttg aaggatatct tgggtgacca
     5281 agtggagaag gttgttgttt cttacaaatt gctagatgct ccagctgcca tcagaactgg
     5341 tcaattcggc tggtctgcta acatggaaag aatcatgaag gctcaagcct tgagagactc
     5401 ttccatgtcc tcctacatgt cttccaagaa gactttcgaa atttctccaa aatctccaat
     5461 tattaaggaa ttgaaaaaga gagttgatga gggtggtgca caagataaga ccgtcaaaga
     5521 tttgactaac ttattattcg agaccgcttt gttgacttct ggtttcagtt tggaagaacc
     5581 aacttctttt gcatcaagaa taaatagatt gatttcttta ggtttgaaca ttgatgagga
     5641 tgaagaaaca gaaaccgctc cagaagcttc taccgaagct ccagttgaag aggttccagc
     5701 tgacaccgag atggaagaag ttgattaatc tcttttttcg ccttcatgtt ttatatatta
     5761 tataaatttg tttacttatt tttactattt gtaataatga ttctgcttta cgcgccttta
     5821 aaaaagttgg tcttaatgta aacatgaacc atagcacaca atttaagaca tcagctgtca
     5881 aatgaccata aaattgtcct accagctgtc cttaaagttt agtgtacact tacgaatgac
     5941 ccatggataa actgattatg ttctaaacac tcctcagaag ctcatcgaac tgtcatcctg
     6001 cgtgaagatt aaaatccaac ttagaaattt cgagcttacg gagacaatca tatgggagaa
     6061 gcaattggaa gatagaaaaa aggtactcgg tacataaata tatgtgattc tgggtagaag
     6121 atcggtctgc attggatggt ggtaacgcat ttttttacac acattacttg cctcgagcat
     6181 caaatggtgg ttattcgtgg atctatatca cgtgatttgc ttaagaattg tcgttcatgg
     6241 tgacactttt agctttgaca tgattaagct catctcaatt gatgttatct aaagtcattt
     6301 caactatcta agatgtggtt gtgattgggc cattttgtga aagccagtac gccagcgtca
     6361 atacactccc gtcaattagt tgcaccatgt ccacaaaatc atataccagt agagctgaga
     6421 ctcatgcaag tccggttgca tcgaaacttt tacgtttaat ggatgaaaag aagaccaatt
     6481 tgtgtgcttc tcttgacgtt cgttcgactg atgagctatt gaaacttgtt gaaacgttgg
     6541 gtccatacat ttgccttttg aaaacacacg ttgatatctt ggatgatttc agttatgagg
     6601 gtactgtcgt tccattgaaa gcattggcag agaaatacaa gttcttgata tttgaggaca
     6661 gaaaattcgc cgatatcggt aacacagtca aattacaata tacatcgggc gtttaccgta
     6721 tcgcagaatg gtctgatatc accaacgccc acggggttac tggtgctggt attgttgctg
     6781 gcttgaaaca aggtgcgcaa gaggtcacca aagaaccaag gggattattg atgcttgctg
     6841 aattgtcttc caagggttct ctagcacacg gtgaatatac taagggtacc gttgatattg
     6901 caaagagtga taaagatttc gttattgggt tcattgctca gaacgatatg ggaggaagag
     6961 aagaagggtt tgattggcta atcatgaccc caggtgtagg tttagacgac aaaggcgatg
     7021 cattgggtca gcagtacaga accgtcgacg aagttgtaag tggtggatca gatatcatca
     7081 ttgttggcag aggacttttc gccaagggta gagatcctaa ggttgaaggt gaaagataca
     7141 gaaatgctgg atgggaagcg taccaaaaga gaatcagcgc tccccattaa ttatacagga
     7201 aacttaatag aacaaatcac atatttaatc taatagccac ctgcattggc acggtgcaac
     7261 actcacttca acttcatctt acaaaagatc acgtgatctg ttgtattggg atctctagac
     7321 ctaataactt cgtatagcat acattatacg aagttatatt aagggttgtc gacctgcagg
     7381 aattcgatat caagcttatc gataccgtcg acctcgaggg ggggcccggt acccagcttt
     7441 tgttcccttt agtgagggtt aattccgagc ttggcgtaat catggtcata gctgtttcct
     7501 gtgtgaaatt gttatccgct cacaattcca cacaacatag gagccggaag cataaagtgt
     7561 aaagcctggg gtgcctaatg agtgaggtaa ctcacattaa ttgcgttgcg ctcactgccc
     7621 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7681 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7741 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7801 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7861 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcg gcccccctga cgagcatcac
     7921 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7981 ttcccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     8041 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat
     8101 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     8161 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     8221 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     8281 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt
     8341 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     8401 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     8461 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     8521 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8581 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8641 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8701 tccatagttg cctgactgcc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8761 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8821 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8881 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8941 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     9001 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgaaaa
     9061 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     9121 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     9181 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     9241 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     9301 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     9361 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     9421 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     9481 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9541 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9601 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9661 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtc
//
