LOCUS       sequence-68166-e         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-synaptobrevin-14-LEXA-QF-hsp70
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             30..50
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             join(60..311,312..665)
                     /codon_start=1
                     /gene="lexA"
                     /product="E. coli DNA-binding protein that represses genes 
                     involved in the SOS response to DNA damage"
                     /label=LexA
                     /note="The N-terminal DNA-binding domain recognizes the 
                     16-bp palindromic sequence 5'-CTGTATATATATACAG-3'."
                     /translation="MKALTARQQEVFDLIRDHISQTGMPPTRAEIAQRLGFRSPNAAEE
                     HLKALARKGVIEIVSGASRGIRLLQEEEEGLPLVGRVAAGEPLLAQQHIEGHYQVDPSL
                     FKPNADFLLRVSGMSMKDIGIMDGDLLAVHKTQDVRNGQVVVARIDDEVTVKRLKKQGN
                     KVELLPENSEFKPIVVDLRQQSFTIEGLAVGVIRNGDWL"
ORIGIN
        1 atcatcgaca gccgaattcc aaaatgccac ccaagaagaa gcgaaaagta gaagatccaa
       61 tgaaggctct cacggcccga caacaggaag tttttgattt gatacgggat catatatccc
      121 aaacgggtat gcctccgacc cgcgcagaga tagcacagcg actgggcttt cgatcgccta
      181 acgccgcgga ggagcacttg aaggcactgg cccgcaaggg tgtcattgaa atcgtgtccg
      241 gtgcgagccg cggaatccgg ctgttgcagg aggaggaaga gggcctgcca ctggtgggac
      301 gcgtggccgc tggcgagccg ctgctggccc agcaacacat agagggacac tatcaggtgg
      361 acccctcctt gtttaagcca aatgctgatt tcctgttgcg ggtgtcggga atgtccatga
      421 aggacatcgg tattatggat ggtgacctcc tcgccgtcca taagacacag gatgtcagga
      481 acggccaggt agtcgttgcc aggatagacg atgaggtcac tgtgaaacgt ctcaagaagc
      541 aaggcaataa ggtcgagctg ctgccggaga atagcgagtt caagccgatc gtggtggatc
      601 tgcgacagca gtcctttact atcgagggct tggccgtggg tgtgatccgc aacggagatt
      661 ggctgggacg tcagttggag ctccctccta ccgccacggc tacggcctcg ataatgccgc
      721 acgtgatgga gcagcctctc agtaccagca ttaaccccgt caacgaccgc ttcaacggta
      781 ttcccaaccc cactccgtat aactccgatg cagctctcga tgctatcact cagaccaacg
      841 attatggaag cgtaaataca catggtatcc tctctactta cccgccac
//
