LOCUS       sequence-68197-e         887 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 887)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 887)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pattB-hsp70P-QF#7-hsp70T
FEATURES             Location/Qualifiers
     source          1..887
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    139..172
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     promoter        190..427
                     /label=hsp70 promoter
                     /note="Drosophila melanogaster hsp70Bb promoter"
     primer_bind     375..396
                     /label=pCasper-hs
                     /note="Drosophila Hsp70 promoter, forward primer"
ORIGIN
        1 gtttgttcct tgtgtagatg catctcaaaa aaatggtggg cataatagtg ttgtttatat
       61 atatcaaaaa taacaactat aataataaga atacatttaa tttagaaaat gcttggattt
      121 cactggaact aggctagcat aacttcgtat aatgtatgct atacgaagtt atgctagcgg
      181 atccgagcgc cggagtataa atagaggcgc ttcgtctacg gagcgacaat tcaattcaaa
      241 caagcaaagt gaacacgtcg ctaagcgaaa gctaagcaaa taaacaagcg cagctgaaca
      301 agctaaacaa tctgcagtaa agtgcaagtt aaagtgaatc aattaaaagt aaccagcaac
      361 caagtaaatc aactgcaact actgaaatct gccaagaagt aattattgaa tacaagaaga
      421 gaactctcga attccaacat gccacccaag cgcaaaacgc ttaacgctgc ggctgaggct
      481 aacgctcatg ccgacggaca cgccgacgga aacgccgacg gacacgtggc caatacggcc
      541 gcgtcctcga ataatgcgag gttcgctgat ctcactaaca tcgatactcc gggtctggga
      601 cccacaacta cgaccctgct cgtggaacca gcacgctcaa agcgtcaacg agtgtcccgc
      661 gcatgcgacc agtgccgtgc agcccgagag aaatgcgacg gaatacagcc tgcgtgtttc
      721 ccgtgcgttt cccagggaag gtcctgcact tatcaggctt cgccgaaaaa gaggggagtt
      781 caaaccggtt atattcgtac gctggagctc gccctcgcct ggatgtttga aaatgtcgcg
      841 cgttccgaag atgccttgca taacctcctc gtccgtgacg ccggaca
//
