LOCUS       sequence-68202-e         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Can be used to make an RNA probe for whole-mount in situ 
            hybridization, for in-frame fusion proteins or as a subcloning step 
            for mRFP. Note that the coding sequence for mRFP lacks the start 
            codon..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Clark KJ, Balciunas D, Pogoda HM, Ding Y, Westcot SE, Bedell VM, 
            Greenwood TM, Urban MD, Skuster KJ, Petzold AM, Ni J, Nielsen AL, 
            Patowary A, Scaria V, Sivasubbu S, Xu X, Hammerschmidt M, Ekker SC
  TITLE     In vivo protein trapping produces a functional expression codex of 
            the vertebrate proteome.
  JOURNAL   Nat Methods. 2011 Jun;8(6):506-15. Epub 2011 May 8.
  PUBMED    21552255
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods."; date: "2011-06"; volume: "8(6)"; pages: "506-15. Epub 
            2011 May 8"
COMMENT     Sequence Label: pCR4-*mRFP
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     1..23
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     20..36
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     20..36
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     promoter        57..75
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     CDS             124..798
                     /codon_start=1
                     /product="monomeric derivative of DsRed (Campbell et al., 
                     2002)"
                     /label=mRFP1
                     /translation="ASSEDVIKEFMRFKVRMEGSVNGHEFEIEGEGEGRPYEGTQTAKL
                     KVTKGGPLPFAWDILSPQFQYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNFEDGGVV
                     TVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASTERMYPEDGALKGEIKMR
                     LKLKDGGHYDAEVKTTYMAKKPVQLPGAYKTDIKLDITSHNEDYTIVEQYERAEGRHST
                     GA"
     primer_bind     complement(251..269)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     519..538
                     /label=mCherry-F
                     /note="mCherry, forward primer"
     primer_bind     713..736
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     primer_bind     complement(841..860)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(842..860)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(867..884)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(867..883)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(876..898)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
ORIGIN
        1 agcggataac aatttcacac aggaaacagc tatgaccatg attacgccaa gctcagaatt
       61 aaccctcact aaagggacta gtcctgcagg tttaaacgaa ttcgccctta agaattcgaa
      121 ggtgcctcct ccgaggatgt catcaaggag ttcatgcgct tcaaggtgcg catggagggc
      181 tccgtgaacg gccacgagtt cgagatcgag ggcgagggcg agggccgccc ctacgagggc
      241 acccagaccg ccaagctgaa ggtgaccaag ggcggccccc tgcccttcgc ctgggacatc
      301 ctgtcccctc agttccagta cggctccaag gcctacgtga agcaccccgc cgacatcccc
      361 gactacttga agctgtcctt ccccgagggc ttcaagtggg agcgcgtgat gaacttcgag
      421 gacggcggcg tggtgaccgt gacccaggac tcctccctgc aggacggcga gttcatctac
      481 aaggtgaagc tgcgcggcac caacttcccc tccgacggcc ccgtaatgca gaagaagacc
      541 atgggctggg aggcctccac cgagcggatg taccccgagg acggcgccct gaagggcgag
      601 atcaagatga ggctgaagct gaaggacggc ggccactacg acgccgaggt caagaccacc
      661 tacatggcca agaagcccgt gcagctgccc ggcgcctaca agaccgacat caagctggac
      721 atcacctccc acaacgagga ctacaccatc gtggaacagt acgagcgcgc cgagggccgc
      781 cactccaccg gagcctaaga ctagtttaag ggcgaattcg cggccgctaa attcaattcg
      841 ccctatagtg agtcgtatta caattcactg gccgtcgttt tacaacgtcg tgactgggaa
      901 aaccctggcg ttacccaact taatcgcctt gcagcacatc cccctttcgc cagctggcgt
      961 aatagcgaag aggcccgcac cgat
//
