LOCUS       sequence-68228-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Seedorf M, Silver PA
  TITLE     Importin/karyopherin protein family members required for mRNA export
            from the nucleus.
  JOURNAL   Proc Natl Acad Sci U S A 1997 Aug 5;94(16):8590-5.
  PUBMED    9238021
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A"; date: "1997-08-5"; volume: "94(16)"; pages: 
            "8590-5"
COMMENT     Sequence Label: pPS1117
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(6..24)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     misc_feature    65..568
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     promoter        605..709
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa tggtttctta
       61 gacggatcgc ttgcctgtaa cttacacgcg cctcgtatct tttaatgatg gaataatttg
      121 ggaatttact ctgtgtttat ttatttttat gttttgtatt tggattttag aaagtaaata
      181 aagaaggtag aagagttacg gaatgaagaa aaaaaaataa acaaaggttt aaaaaatttc
      241 aacaaaaagc gtactttaca tatatattta ttagacaaga aaagcagatt aaatagatat
      301 acattcgatt aacgataagt aaaatgtaaa atcacaggat tttcgtgtgt ggtcttctac
      361 acagacaaga tgaaacaatt cggcattaat acctgagagc aggaagagca agataaaagg
      421 tagtatttgt tggcgatccc cctagagtct tttacatctt cggaaaacaa aaactatttt
      481 ttctttaatt tcttttttta ctttctattt ttaatttata tatttatatt aaaaaattta
      541 aattataatt atttttatag cacgtgatga aaaggaccca ggtggcactt ttcggggaaa
      601 tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat
      661 gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca
      721 acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca
      781 cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta
      841 catcgaactg gatctc
//
