LOCUS       sequence-6824-ex       10493 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10493)
  AUTHORS   Meister G, Landthaler M, Peters L, Chen PY, Urlaub H, Luhrmann R, 
            Tuschl T
  TITLE     Identification of novel argonaute-associated proteins.
  JOURNAL   Curr Biol. 2005 Dec 6;15(23):2149-55. doi: 
            10.1016/j.cub.2005.10.048. Epub 2005 Nov 10.
  PUBMED    16289642
REFERENCE   2  (bases 1 to 10493)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cub.2005.10.048"; journalName: "Curr Biol"; date: 
            "2005-12-6- 6"; volume: "15"; issue: "23"; pages: "2149-55"
COMMENT     Sequence Label: pFLAG/HA-TNRC6B
FEATURES             Location/Qualifiers
     source          1..10493
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(44..63)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        235..614
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        615..818
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     769..789
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     863..882
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        863..881
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      946..955
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             955..978
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     CDS             979..1005
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             6265..6306
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     intron          6387..6616
                     /label=chimeric intron
                     /note="chimera between introns from adenovirus and 
                     immunoglobulin heavy chain genes"
     primer_bind     6599..6618
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     misc_feature    join(6674..7248,7249..7251,7252..7260)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6841..6858)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7068..7087
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             7280..7879
                     /codon_start=1
                     /gene="pac from Streptomyces"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVECPKDRATWCMTRKPGA"
     primer_bind     complement(7280..7299)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     7776..7796
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     polyA_signal    8113..8234
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8150..8169)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(8336..8352)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8336..8352)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8349..8371)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8360..8376
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8384..8390,8391..8408,8409..8414))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8429..8450
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8567..8584)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8738..9326)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8818..8837)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9497..10288,10289..10357))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10120..10139
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10358..10462)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtcgactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggac tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gcttggtacc
      901 gagctcggat cgatccatcg atagtgtggt ggaattctga tcgccgccgc catggactac
      961 aaggacgacg atgacaagta cccttatgac gtgcccgatt acgctttgaa ccagatatca
     1021 acaagtttat gagagagaag gagcaagaaa gggaagaaca gttaatggaa gacaagaaaa
     1081 ggaagaaaga ggataaaaag aaaaaagaag ccactcagaa ggtcacggaa caaaaaacca
     1141 aagtgcccga agtgacgaaa ccaagtttaa gccaaccaac ggccgccagc ccaattggca
     1201 gctctccatc gccaccagtc aatggtggca acaatgccaa aagggtggca gtgccgaacg
     1261 gacaaccgcc aagcgccgcc cgctacatgc ctcgggaggt gccgccgcga ttccgttgcc
     1321 agcaggacca caaagtgtta ctaaaacgtg ggcagccccc tccaccgtcc tgcatgctcc
     1381 ttgggggtgg ggcagggcct cctccctgca cagcacctgg agcaaaccca aacaacgcac
     1441 aagtgacagg agcgctgctg cagagtgaga gtgggactgc gccagactca acccttggag
     1501 gtgctgctgc ttcaaattat gcaaattcca cttggggctc gggagcctcc tccaacaacg
     1561 gcacctcccc caacccaatt cacatctggg acaaggtgat tgtagacggg tctgacatgg
     1621 aagagtggcc ttgtattgcc agcaaagaca ctgaatcttc ttccgaaaac accaccgata
     1681 acaacagtgc ctcgaaccct ggctctgaga agagcactct gccaggaagc accactagta
     1741 acaaaggaaa agggagccag tgccagtctg caagttctgg gaacgaatgt aatcttgggg
     1801 tctggaaatc tgaccctaag gctaaatctg ttcaatcttc caactctact acagagaaca
     1861 acaatggact aggaaattgg aggaatgtga gtggtcagga tagaattgga cctggctctg
     1921 gcttcagcaa ctttaaccca aatagcaacc catctgcctg gccagcactg gtccaagaag
     1981 gaacttctag gaaaggggca ttggaaacag ataatagtaa ttccagtgca caggttagca
     2041 cagtaggtca gacatccagg gaacagcagt caaagatgga aaatgcgggt gttaattttg
     2101 ttgtctctgg cagagaacag gctcaaattc ataacactga tggaccaaaa aatggaaaca
     2161 ctaactcctt gaacttaagt tcaccaaacc ccatggagaa taagggaatg ccctttggaa
     2221 tgggcttggg gaacacctcc aggagcactg atgccccttc acaaagcact ggagatcgaa
     2281 agactgggag tgttggatct tggggtgcag ctagggggcc ttctggaact gacacagtct
     2341 ctggacaaag caattctgga aacaatggga acaatggaaa agagagagag gactcctgga
     2401 aaggagcttc tgttcagaaa tcaactgggt caaaaaatga ctcttgggac aacaataaca
     2461 ggtctacggg tgggtcctgg aactttggcc cccaggactc taatgacaac aaatggggtg
     2521 aagggaacaa aatgacatct ggggtctctc agggagaatg gaaacagccg actgggtctg
     2581 atgagttgaa aattggagaa tggagtggtc caaaccaacc aaattctagc actggagcat
     2641 gggacaatca aaagggccac cccctccctg aaaaccaagg caatgcccag gctccctgtt
     2701 ggggaagatc ttccagctcc acaggaagtg aagttggagg tcaaagcact ggaagcaacc
     2761 acaaagcagg aagtagtgac agtcataact ctggccgtcg gtcgtacagg cccacacatc
     2821 ctgattgtca ggctgtcttg cagactcttt tgagccgaac tgatttggac cccagggtgc
     2881 tctcaaacac tggctggggc caaactcaaa ttaagcagga cacagtgtgg gacattgaag
     2941 aggtgccaag gcctgagggg aaatctgaca aaggaactga ggggtgggag agcgctgcca
     3001 cacagaccaa gaactcaggg ggctggggag atgcacccag ccaaagcaat caaatgaagt
     3061 ctggatgggg ggagctctca gcctctacag agtggaaaga ccccaagaac acaggaggct
     3121 ggaatgacta caagaacaac aactcttcca actggggagg aggacgacct gatgaaaaga
     3181 caccttcctc ttggaatgag aatcccagca aggatcaggg gtggggaggt ggacgccagc
     3241 ccaatcaagg atggtcttct ggaaagaatg gttgggggga ggaagtcgat cagacaaaaa
     3301 acagcaattg ggaaagttct gcaagtaaac ctgtgtctgg gtggggtgaa ggagggcaga
     3361 atgaaatcgg gacttggggt aatggtggca atgcaagcct agcttcaaaa ggtgggtggg
     3421 aggattgcaa aagatcccca gcatggaatg agacgggccg acagcccaat tcctggaata
     3481 aacaacacca acagcagcag cccccacagc agccgccgcc accacaacca gaggcttctg
     3541 gttcgtgggg aggcccaccc ccaccacctc caggcaacgt tcgaccttcc aattccagct
     3601 ggagcagcgg gccacagcct gcaacaccta aggatgagga acccagtggt tgggaagagc
     3661 catccccaca gtcaattagt cggaaaatgg acattgatga tggcacttca gcatggggag
     3721 accctaacag ttataactac aagaatgtga atctgtggga taagaattcc caagggggcc
     3781 cagcacctcg agaaccaaac ctgcccaccc caatgaccag taaatcggca tcagattcca
     3841 aatctatgca agacggctgg ggggagagtg acgggccagt cacaggagct cgccatccca
     3901 gctgggaaga ggaggaggat ggaggagtct ggaacaccac tggctctcag ggcagtgctt
     3961 cctcccacaa ctcagcaagc tggggacaag gaggaaagaa acaaatgaag tgctcactca
     4021 aaggaggaaa caatgattca tggatgaatc ctcttgccaa acagttttca aatatgggat
     4081 tgctgagtca gactgaagat aatccaagca gcaaaatgga tttgtctgta ggaagccttt
     4141 cagataaaaa atttgatgtg gacaagcgag cgatgaatct cggggatttt aatgatatca
     4201 tgaggaagga tcgatctggg ttccgtccac ctaattccaa agacatggga accacagata
     4261 gtgggcctta ttttgagaag ggcggtagtc atggtttgtt tggaaacagc acagcacaat
     4321 cgagaggtct gcacacaccc gtgcagccac taaattcttc tcccagtctc cgggcgcaag
     4381 tgcctcccca gtttatttcc ccccaggttt ctgcctcaat gctcaagcag tttcccaaca
     4441 gtggcctgag tccaggtctt ttcaatgtgg ggccccagtt atctcctcaa caaattgcca
     4501 tgctgagcca gcttccacaa attccccagt ttcagttggc atgtcagctt ctcttgcagc
     4561 agcagcaaca gcagcagttg ttacagaacc agagaaagat ttctcaagct gtacgccaac
     4621 agcaagagca gcagctggct cgaatggtga gtgcactgca gcagcagcag cagcagcaga
     4681 ggcagccagg catgaagcac tcgccctctc atcctgttgg gcccaagccg catctggaca
     4741 acatggtacc caacgcattg aatgtggggc tcccagacct tcaaaccaaa gggccaatac
     4801 ctggatatgg ttctggcttc agctctggcg gcatggacta tggcatggtt ggtgggaagg
     4861 aggctggaac cgagtctcgc tttaaacagt ggacctccat gatggagggg ctgccctctg
     4921 tagccacaca ggaagccaat atgcacaaaa atggcgctat agtggcccct ggtaaaaccc
     4981 ggggagggtc accgtacaac cagtttgata tcatccctgg tgacacactg ggtggccata
     5041 cgggtcctgc tggtgatagc tggttacctg ccaaatctcc accaacaaat aaaatcggaa
     5101 gtaaatccag caatgccagt tggcctccag aattccaacc aggagtgcca tggaaaggta
     5161 tccaaaacat tgaccctgaa tctgacccct atgtcacccc aggaagtgtg ctggggggta
     5221 cagccacatc tcccattgta gatactgacc accaactgct gcgggataac accacagggt
     5281 ctaattcttc cctcaacacc tcgctgcctt cacctggtgc ctggccctac agtgcctctg
     5341 acaactcctt taccaacgtt catagcactt cagcaaagtt ccctgattac aaatcaacat
     5401 ggtccccaga tcccatagga cacaacccca ctcatctctc caacaagatg tggaaaaacc
     5461 atatttcctc caggaacact acaccgctgc cccgcccacc tcctggtctg accaacccca
     5521 aaccatcatc tccctggagc agcacagcac cccgatcagt cagggggtgg gggacacagg
     5581 actcacggct cgcctcggcc tctacctgga gtgatggtgg ctcagttcgt cctagttact
     5641 ggctggttct tcacaatctc accccacaga ttgatgggtc aaccttgaga acgatctgca
     5701 tgcagcatgg cccactgctg acattccatc tgaatctaac ccagggcact gccctgatcc
     5761 gatacagcac caaacaggag gcggccaagg cccaaactgc actgcacatg tgtgtgttgg
     5821 gaaacactac catccttgct gagtttgcca ctgatgatga agtcagccgc tttctggcac
     5881 aagctcagcc ccctacacct gcagcaaccc caagtgcgcc agctgcgggg tggcagtcgc
     5941 tggagaccgg ccagaaccag tcagatcccg tgggacctgc tctgaatctt tttggtgggt
     6001 ccactgggct cgggcagtgg agcagcagcg ctggtggcag cagcggggcc gatcttgctg
     6061 gcgcttcatt gtgggggccc ccaaactatt cttctagctt atggggagtc ccaacggtgg
     6121 aagatcccca taggatgggc agccctgctc ctttactacc tggtgacctt ctgggaggag
     6181 ggtcggattc aatctgactt gtacaaagtg gttgatatcc agcacagtgg cggccgctca
     6241 agtctagagg gcccgcggtt cgaaggtaag cctatcccta accctctcct cggtctcgat
     6301 tctacgcgta ccggatccgc ggccgcatag ataactgatc cagtgtgctg gaattaattc
     6361 gctgtctgcg agggccagct gttggggtga gtactccctc tcaaaagcgg gcatgacttc
     6421 tgcgctaaga ttgtcagttt ccaaaaacga ggaggatttg atattcacct ggcccgcggt
     6481 gatgcctttg agggtggccg cgtccatctg gtcagaaaag acaatctttt tgttgtcaag
     6541 cttgaggtgt ggcaggcttg agatctggcc atacacttga gtgacaatga catccacttt
     6601 gcctttctct ccacaggtgt ccactcccag gtccaactgc aggtcgagca tgcatctagg
     6661 gcggccaatt ccgcccctct ccctcccccc cccctaacgt tactggccga agccgcttgg
     6721 aataaggccg gtgtgcgttt gtctatatgt gattttccac catattgccg tcttttggca
     6781 atgtgagggc ccggaaacct ggccctgtct tcttgacgag cattcctagg ggtctttccc
     6841 ctctcgccaa aggaatgcaa ggtctgttga atgtcgtgaa ggaagcagtt cctctggaag
     6901 cttcttgaag acaaacaacg tctgtagcga ccctttgcag gcagcggaac cccccacctg
     6961 gcgacaggtg cctctgcggc caaaagccac gtgtataaga tacacctgca aaggcggcac
     7021 aaccccagtg ccacgttgtg agttggatag ttgtggaaag agtcaaatgg ctctcctcaa
     7081 gcgtattcaa caaggggctg aaggatgccc agaaggtacc ccattgtatg ggatctgatc
     7141 tggggcctcg gtgcacatgc tttacatgtg tttagtcgag gttaaaaaaa cgtctaggcc
     7201 ccccgaacca cggggacgtg gttttccttt gaaaaacacg atgataagct tgccacaacc
     7261 cacaaggaga cgaccttcca tgaccgagta caagcccacg gtgcgcctcg ccacccgcga
     7321 cgacgtcccc cgggccgtac gcaccctcgc cgccgcgttc gccgactacc ccgccacgcg
     7381 ccacaccgtc gacccggacc gccacatcga gcgggtcacc gagctgcaag aactcttcct
     7441 cacgcgcgtc gggctcgaca tcggcaaggt gtgggtcgcg gacgacggcg ccgcggtggc
     7501 ggtctggacc acgccggaga gcgtcgaagc gggggcggtg ttcgccgaga tcggcccgcg
     7561 catggccgag ttgagcggtt cccggctggc cgcgcagcaa cagatggaag gcctcctggc
     7621 gccgcaccgg cccaaggagc ccgcgtggtt cctggccacc gtcggcgtct cgcccgacca
     7681 ccagggcaag ggtctgggca gcgccgtcgt gctccccgga gtggaggcgg ccgagcgcgc
     7741 cggggtgccc gccttcctgg agacctccgc gccccgcaac ctccccttct acgagcggct
     7801 cggcttcacc gtcaccgccg acgtcgagtg cccgaaggac cgcgcgacct ggtgcatgac
     7861 ccgcaagccc ggtgcctgac gcccgcccca cgacccgcag cgcccgaccg aaaggagcgc
     7921 acgaccccat ggctccgacc gaagccgacc cgggcggccc cgccgacccc gcacccgccc
     7981 ccgaggccca ccgactctag ataactgatc ataatcagcc ataccacatt tgtagaggtt
     8041 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
     8101 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
     8161 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
     8221 atcaatgtat cttaacgcgt cgagtgcatt ctagttgtgg tttgtccaaa ctcatcaatg
     8281 tatcttatca tgtctgtata ccgtcgacct ctagctagag cttggcgtaa tcatggtcat
     8341 agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa
     8401 gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc
     8461 gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc
     8521 aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact
     8581 cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac
     8641 ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa
     8701 aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc cgcccccctg
     8761 acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa
     8821 gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc
     8881 ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct caatgctcac
     8941 gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac
     9001 cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg
     9061 taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt
     9121 atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga
     9181 cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct
     9241 cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga
     9301 ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg
     9361 ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct
     9421 tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt
     9481 aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc
     9541 tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg
     9601 gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag
     9661 atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt
     9721 tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag
     9781 ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca cgctcgtcgt
     9841 ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca
     9901 tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg
     9961 ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat
    10021 ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta
    10081 tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca
    10141 gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct
    10201 taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat
    10261 cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa
    10321 agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt
    10381 gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa
    10441 ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac gtc
//
