LOCUS       sequence-68245-e         755 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 755)
  AUTHORS   Riabinina O, Luginbuhl D, Marr E, Liu S, Wu MN, Luo L, Potter CJ
  TITLE     Improved and expanded Q-system reagents for genetic manipulations.
  JOURNAL   Nat Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250.
  PUBMED    25581800
REFERENCE   2  (bases 1 to 755)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat 
            Methods. 2015 Jan 12. doi: 10.1038/nmeth.3250."
COMMENT     Sequence Label: pAC-4-QFBDMD-G4AD
FEATURES             Location/Qualifiers
     source          1..755
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(71..415)
                     /codon_start=1
                     /gene="Saccharomyces cerevisiae GAL4 (truncated)"
                     /product="activation domain of the GAL4 transcriptional 
                     activator"
                     /label=GAL4 activation domain
                     /translation="ANFNQSGNIADSSLSFTFTNSSNGPNLITTQTNSQALSQPIASSN
                     VHDNFMNNEITASKIDDGNNSKPLSPGWTDQTAYNAFGITTGMFNTTTMDDVYNYLFDD
                     EDTPPNPKKE"
ORIGIN
        1 gacagagtac ttgcgctctg gcggggcaat gatcttgatc ttcatggtcg acggatcggt
       61 taacgcggcc ttactctttt tttgggtttg gtggggtatc ttcatcatcg aatagatagt
      121 tatatacatc atccattgta gtggtattaa acatccctgt agtgattcca aacgcgttat
      181 acgcagtttg gtccgtccaa ccaggtgaca gtggttttga attattacca tcatcaattt
      241 tactagccgt gatttcatta ttcatgaagt tatcatgaac gttagaggag gcaattggtt
      301 gtgaaagcgc ttgagaattt gtttgagttg ttatgaggtt cggaccgttg ctactgttag
      361 tgaaagtgaa ggacaatgag ctatcagcaa tattcccact ttgattaaaa ttggcgccac
      421 caaacaaagc agacggggtc agtggaattg gctgcagacc agtgtttgaa tgccttccac
      481 ctcgcggcca gatagacgaa taagccggaa atagctcctc caggtggcgc ctgtgacgct
      541 ccgacgaatg gctctgcttt ttgagagcca ggagcgagtc gaggagcgac ggaataaggg
      601 gcggaatggc atatgttccg aagcgttttt ggtactggtc gagcgtctca atcaagaggt
      661 gggaataatg ctcattcagg ggttcgctga acgccgccgc ccagaggtaa acagtacgag
      721 tcagataagc cgtcgggacg aaagcgcttg ccgtg
//
