LOCUS       sequence-68256-e         883 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 883)
  AUTHORS   Ruas JL, White JP, Rao RR, Kleiner S, Brannan KT, Harrison BC, 
            Greene NP, Wu J, Estall JL, Irving BA, Lanza IR, Rasbach KA, Okutsu 
            M, Nair KS, Yan Z, Leinwand LA, Spiegelman BM
  TITLE     A PGC-1alpha isoform induced by resistance training regulates 
            skeletal muscle hypertrophy.
  JOURNAL   Cell. 2012 Dec 7;151(6):1319-31. doi: 10.1016/j.cell.2012.10.050.
  PUBMED    23217713
REFERENCE   2  (bases 1 to 883)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.cell.2012.10"; journalName: "Cell"; date: "2012-12-7- 7";
            volume: "151"; issue: "6"; pages: "1319-31"
COMMENT     Sequence Label: pcDNA3.1-Flag-PGC-1alpha2
FEATURES             Location/Qualifiers
     source          1..883
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     35..54
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        35..53
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctagagaacc cactgcttac tggcttatcg aaattaatac gactcactat agggagaccc
       61 aagcttggta ccgagctcgg atccaccatg gactacaaag acgatgacga caaggcggcc
      121 gcgatgttgg gattgtcatc catggattca attttgaaat gtgctgctct ggttggtgag
      181 gaccagcctc tttgcccaga tcttcctgaa cttgaccttt ctgaacttga tgtgaatgac
      241 ttggatacag acagctttct gggtggattg aagtggtgta gcgaccaatc ggaaatcata
      301 tccaaccagt acaacaatga gcctgcgaac atatttgaga agatagatga agagaatgag
      361 gcaaacttgc tagcggttct cacagagaca ctggacagtc tccccgtgga tgaagacgga
      421 ttgccctcat ttgagaacaa gactattgag cgaaccttaa gtgtggaact ctctggaact
      481 gcaggcctaa ctcctcccac aactcctcct cataaagcca accaagataa ccctttcaag
      541 gcttcgccaa agctgaagcc ctcttgcaag accgtggtgc caccgccaac caagagggcc
      601 cggtacagtg agtgttctgg tacccaaggc agccactcca ccaagaaagg gcccgagcaa
      661 tctgagttgt acgcacaact cagcaagtcc tcagggctca gccgaggaca cgaggaaagg
      721 aagactaaac ggcccagtct ccggctgttt ggtgaccatg actactgtca gtcactcaat
      781 tccaaaacgg atatactcat taacatatca caggagctcc aagactctag acaactagac
      841 ttcaaagatg cctcctgtga ctggcaggga cacatctgtt ctt
//
