LOCUS       sequence-68278-e         882 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express Fun30 in trans in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 882)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 882)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA1
FEATURES             Location/Qualifiers
     source          1..882
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(15..32)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(42..83)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 cctctagaaa ctcaatggtg atggtgatga tgaccggtac gcgtagaatc gagaccgagg
       61 agagggttag ggataggctt accttcgaat gggtgacctc gaagctcgcc cttctcgagt
      121 tctttggttc ccttcggttt cgagttttca tcataaatta tatcctccaa catatcacta
      181 actttacttt ccaacacatc ttgagatttt ttatcttcac tgatatacga atctaaagct
      241 agtttatttt tggccagttg atgaatcttt tcctctatgg aatccttagt aattaaagtg
      301 gttatattaa cttcctttgt ttgtcccaca cgatgtgccc tatcagcagc ttgtctgtca
      361 tcatgtgggt taaaactttg atcgaatata ataacattat ttgcgcacac caaattaata
      421 ccgaatccac ctgcctttgt tgataagatg aaaatgggaa tatccttatc ttcataaaac
      481 ttatctatta gtagttgtct atcattcact tgcgtggaac catctaatct taaaaattta
      541 taatctaagg tggacaaaac catctctaga atatccagga cttgagtgaa taaggaaaat
      601 atcagcacct tttcctgttt gtcaacaatg attgttttca gcaatttttt caaagcgtct
      661 atcttcccag attgcatcca ctcgtcatta tgaagttggt atttggataa cgtgttcggg
      721 aaattgcagc atagtttgtg caactcaaaa tccgtcatat agctcatatc ttccttaata
      781 tactctttgt taccgttttc agcataagca ggttcatcca atatggcatc actcatttta
      841 gtgatgattt tatcattata gatatttctg aacaaaagtg ga
//
