LOCUS       sequence-6828-ex       11082 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11082)
  TITLE     Unpublished, Paco
REFERENCE   2  (bases 1 to 11082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pRS415-SPT6
FEATURES             Location/Qualifiers
     source          1..11082
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     complement(29..51)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     151..170
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     CDS             complement(663..1757)
                     /codon_start=1
                     /gene="S. cerevisiae LEU2"
                     /product="3-isopropylmalate dehydrogenase, required for 
                     leucine biosynthesis"
                     /label=LEU2
                     /note="yeast auxotrophic marker"
                     /translation="MSAPKKIVVLPGDHVGQEITAEAIKVLKAISDVRSNVKFDFENHL
                     IGGAAIDATGVPLPDEALEASKKVDAVLLGAVGGPKWGTGSVRPEQGLLKIRKELQLYA
                     NLRPCNFASDSLLDLSPIKPQFAKGTDFVVVRELVGGIYFGKRKEDDGDGVAWDSEQYT
                     VPEVQRITRMAAFMALQHEPPLPIWSLDKANVLASSRLWRKTVEETIKNEFPTLKVQHQ
                     LIDSAAMILVKNPTHLNGIIITSNMFGDIISDEASVIPGSLGLLPSASLASLPDKNTAF
                     GLYEPCHGSAPDLPKNKVDPIATILSAAMMLKLSLNLPEEGKAIEDAVKKVLDAGIRTG
                     DLGGSNSTTEVGDAVAEEVKKILA"
     promoter        complement(1770..2174)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     rep_origin      complement(2474..2929)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(2611..2632)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     2823..2842
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3059..3081
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     3073..3090
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     3074..3090
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     3100..3119
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        3100..3118
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     3143..3159
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     3144..3160
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8255..8271)
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8255..8271)
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(8308..8328)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(8308..8326)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(8347..8363)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8347..8363)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8360..8382)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8371..8387
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8395..8401,8402..8419,8420..8425))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     primer_bind     complement(8578..8595)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8749..9337)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8829..8848)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9508..10299,10300..10368))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10131..10150
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10369..10473)
                     /gene="bla"
                     /label=AmpR promoter
     misc_feature    10510..11013
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     11055..11073
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
ORIGIN
        1 tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat gcagctcccg gagacggtca
       61 cagcttgtct gtaagcggat gccgggagca gacaagcccg tcagggcgcg tcagcgggtg
      121 ttggcgggtg tcggggctgg cttaactatg cggcatcaga gcagattgta ctgagagtgc
      181 accatatcga ctacgtcgta aggccgtttc tgacagagta aaattcttga gggaactttc
      241 accattatgg gaaatggttc aagaaggtat tgacttaaac tccatcaaat ggtcaggtca
      301 ttgagtgttt tttatttgtt gtattttttt ttttttagag aaaatcctcc aatatcaaat
      361 taggaatcgt agtttcatga ttttctgtta cacctaactt tttgtgtggt gccctcctcc
      421 ttgtcaatat taatgttaaa gtgcaattct ttttccttat cacgttgagc cattagtatc
      481 aatttgctta cctgtattcc tttactatcc tcctttttct ccttcttgat aaatgtatgt
      541 agattgcgta tatagtttcg tctaccctat gaacatattc cattttgtaa tttcgtgtcg
      601 tttctattat gaatttcatt tataaagttt atgtacaaat atcataaaaa aagagaatct
      661 ttttaagcaa ggattttctt aacttcttcg gcgacagcat caccgacttc ggtggtactg
      721 ttggaaccac ctaaatcacc agttctgata cctgcatcca aaaccttttt aactgcatct
      781 tcaatggcct taccttcttc aggcaagttc aatgacaatt tcaacatcat tgcagcagac
      841 aagatagtgg cgatagggtc aaccttattc tttggcaaat ctggagcaga accgtggcat
      901 ggttcgtaca aaccaaatgc ggtgttcttg tctggcaaag aggccaagga cgcagatggc
      961 aacaaaccca aggaacctgg gataacggag gcttcatcgg agatgatatc accaaacatg
     1021 ttgctggtga ttataatacc atttaggtgg gttgggttct taactaggat catggcggca
     1081 gaatcaatca attgatgttg aaccttcaat gtagggaatt cgttcttgat ggtttcctcc
     1141 acagtttttc tccataatct tgaagaggcc aaaacattag ctttatccaa ggaccaaata
     1201 ggcaatggtg gctcatgttg tagggccatg aaagcggcca ttcttgtgat tctttgcact
     1261 tctggaacgg tgtattgttc actatcccaa gcgacaccat caccatcgtc ttcctttctc
     1321 ttaccaaagt aaatacctcc cactaattct ctgacaacaa cgaagtcagt acctttagca
     1381 aattgtggct tgattggaga taagtctaaa agagagtcgg atgcaaagtt acatggtctt
     1441 aagttggcgt acaattgaag ttctttacgg atttttagta aaccttgttc aggtctaaca
     1501 ctaccggtac cccatttagg accacccaca gcacctaaca aaacggcatc aaccttcttg
     1561 gaggcttcca gcgcctcatc tggaagtggg acacctgtag catcgatagc agcaccacca
     1621 attaaatgat tttcgaaatc gaacttgaca ttggaacgaa catcagaaat agctttaaga
     1681 accttaatgg cttcggctgt gatttcttga ccaacgtggt cacctggcaa aacgacgatc
     1741 ttcttagggg cagacatagg ggcagacatt agaatggtat atccttgaaa tatatatata
     1801 tattgctgaa atgtaaaagg taagaaaagt tagaaagtaa gacgattgct aaccacctat
     1861 tggaaaaaac aataggtcct taaataatat tgtcaacttc aagtattgtg atgcaagcat
     1921 ttagtcatga acgcttctct attctatatg aaaagccggt tccggcctct cacctttcct
     1981 ttttctccca atttttcagt tgaaaaaggt atatgcgtca ggcgacctct gaaattaaca
     2041 aaaaatttcc agtcatcgaa tttgattctg tgcgatagcg cccctgtgtg ttctcgttat
     2101 gttgaggaaa aaaataatgg ttgctaagag attcgaactc ttgcatctta cgatacctga
     2161 gtattcccac agttaactgc ggtcaagata tttcttgaat caggcgcctt agaccgctcg
     2221 gccaaacaac caattacttg ttgagaaata gagtataatt atcctataaa tataacgttt
     2281 ttgaacacac atgaacaagg aagtacagga caattgattt tgaagagaat gtggattttg
     2341 atgtaattgt tgggattcca tttttaataa ggcaataata ttaggtatgt ggatatacta
     2401 gaagttctcc tcgaccgtcg atatgcggtg tgaaataccg cacagatgcg taaggagaaa
     2461 ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt aaatttttgt
     2521 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
     2581 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
     2641 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
     2701 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
     2761 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
     2821 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
     2881 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc gcgccattcg
     2941 ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc
     3001 cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc
     3061 cagtcacgac gttgtaaaac gacggccagt gagcgcgcgt aatacgactc actatagggc
     3121 gaattgggta ccgggccccc cctcgaggtc gacggtatcg ataagcttga tatcgaattc
     3181 ctgcagcccc gttacgaaga acagaaattc aatgttctca ggacgtgctg aaaggaaacc
     3241 cacagaaagc agacattctg ttgcaaagaa aactgaaaaa aaaataaata caaggccccc
     3301 cttcagatga ctacgcccaa cccaaccaaa gaattatcct cctcacaaaa tatatcgcta
     3361 gcatgcgtga gttatggata agaagaatat ttgatgagct gactgattct ttatagcaat
     3421 agggaggaag gcagtacaca tatcagcagt gcatgggcac aacccgcatt acaacgaaac
     3481 atgatatatc tatataaaag aagatacgga caggcggtcg aggctattac tgtgccacta
     3541 tgcgagtcat gaatgatttt taaattagtt agtacccctt tatataacgt cttcaataaa
     3601 agtgcgggaa aattcgggta atattttcta taactgtcat ttttttttcg ttatgtatgg
     3661 caaaccaaat tgacaagaaa ctggacaacg gttaaagcaa aaggaggaag aaagatattg
     3721 atttgtgctt tgaagaataa cccaagcgaa caggtcatta attgcctaga atctttacat
     3781 agaatttgcc ttttatggaa gagacgggag attcgaagct ggtccctagg gacgaggaag
     3841 aaatagtaaa tgacaacgat gaaactaaag cgcctagtga ggaagaagaa ggagaagatg
     3901 tctttgactc ctctgaggaa gacgaagata ttgatgaaga cgaagatgag gcaagaaaag
     3961 tgcaagaagg cttcattgtc aacgatgatg atgaaaatga agacccagga acaagtattt
     4021 ccaaaaaaag aagaaaacat aaaagaagag aaagagaaga agatgatcga ctatccgaag
     4081 atgatttgga tttgttaatg gagaatgctg gtgttgaacg taccaaagcg agctcctctt
     4141 caggaaaatt taaaagatta aagagagtag gagatgaagg aaacgctgca gaatcagaaa
     4201 gtgacaacgt cgctgcatca aggcaagatt ctacgtctaa attggaagat ttcttttctg
     4261 aagatgaaga agaagaagaa tctggtttac gcaacggtag gaacaatgaa tatgggcgtg
     4321 atgaagaaga ccatgaaaat aggaacagaa cagctgacaa aggtggaatc ttggacgaat
     4381 tggatgattt cattgaagat gatgaatttt ctgatgagga cgacgaaacc agacaaagga
     4441 ggatccaaga aaagaagctt ttaagagaac aatccatcaa acaacctaca cagattactg
     4501 gtctatcgtc ggataagatt gacgagatgt atgacatttt tggtgatggt catgactacg
     4561 attgggcttt agaaattgaa aatgaagaac tagaaaatgg taacgacaac aatgaagctg
     4621 aagaagaaga gatcgatgaa gaaactggtg ctataaaaag caccaagaaa aagatatctc
     4681 tacaagacat ttatgattta gaggatttga aaaaaaactt gatgactgaa ggagacatga
     4741 aaattagaaa gacagatatt ccagaaagat atcaagaatt aagagcaggt attactgact
     4801 acggaaatat gtcatcggag gatcaagaat tagaaagaaa ctggatagca gaaaaaattt
     4861 ctgtggataa gaacttcgat gccaattatg atctcactga atttaaagaa gcaattggga
     4921 acgcaatcaa atttatcacc aaagaaaact tggaagtccc ttttatatat gcttaccgtc
     4981 gtaactatat ttcctcaaga gaaaaagatg ggtttctttt gactgaagat gacctttggg
     5041 atatagttag ccttgacatc gaatttcaca gtcttgtgaa caaaaaggat tatgtccaga
     5101 gattttatgc agaattacat atcgatgatc ctattgtcac tgaatacttc aaaaatcaga
     5161 atactgcatc tattgcggaa ctgaattctc tacaggatat ttatgactac ctagaattca
     5221 aatatgccaa cgaaatcaat gaaatgttta taaaccacac tggaaagact ggtaagaaac
     5281 atttgaaaaa ttccagttat gaaaaattta aagctagtcc tctttatcaa gcggttagtg
     5341 atattggtat atcagctgag gatgttggtg aaaatatcag ttcccagcat caaatccacc
     5401 ctcccgtaga tcatccaagt tccaaaccag tagaagtgat agaatctata ttgaatgcaa
     5461 acagcggtga tttgcaagtc tttacgtcca atactaagct ggcaattgat acggtccaaa
     5521 aatactactc tttggaattg tctaaaaata caaaaataag ggaaaaagtt agatccgatt
     5581 tttccaaata ttatctggct gacgttgtgt taactgctaa aggtaaaaaa gaaattcaaa
     5641 agggatctct gtatgaggac ataaaatatg ccatcaatag aactccaatg cacttccgta
     5701 gggatccaga cgtttttttg aaaatggtcg aggctgagtc tttgaacctg ctcagtgtta
     5761 agttacacat gtcgtcacaa gcccaatata tagagcattt attccaaatt gcacttgaaa
     5821 ctaccaatac ctcggacatc gcaatagaat ggaataactt ccgtaaactg gcattcaacc
     5881 aagcgatgga caagattttc caggatatat ctcaagaagt caaagacaat ttaacaaaaa
     5941 attgtcaaaa attggtagcc aagactgttc gccataagtt tatgacaaaa ttagaccagg
     6001 ctccattcat tcctaatgtc agggatccaa aaattccaaa aatcttatct ttaacctgtg
     6061 gacagggtag attcggagcc gacgctataa ttgctgtcta cgtcaacaga aagggtgatt
     6121 ttataagaga ttacaagatt gtcgacaatc catttgataa gacgaatcct gaaaaatttg
     6181 aagacacctt ggataatatc attcaaagct gtcaaccgaa tgccatcgga atcaatggcc
     6241 ctaacccaaa gactcaaaaa ttttacaaaa gattacaaga agttctacat aagaagcaaa
     6301 tcgtcgacag tagaggacat actattccaa tcatttacgt tgaggacgaa gtcgctatcc
     6361 gttatcagaa ttccgaaaga gctgctcaag aattccctaa taaacctcct ctagttaaat
     6421 actgtatcgc cttggcgcgc tatatgcatt ccccattgtt ggaatatgct aatttaacaa
     6481 gtgaagaagt gagatcattg tcaattcatc cacaccaaaa tctgttatcc tcagaacaat
     6541 tgagttgggc tcttgaaacc gctttcgttg atattgtcaa cctggtaagt gttgaagtta
     6601 acaaagccac agataataat tactacgcta gtgcgctgaa atacatctct ggctttggaa
     6661 aacgtaaagc tattgatttc ttacagtccc ttcaaaggct aaatgaacca ttactggctc
     6721 gtcaacaatt aattactcat aacattcttc acaagactat ttttatgaat tccgcgggat
     6781 tcctctatat ctcatggaat gaaaaaagac aaaaatacga agatttggaa catgatcaac
     6841 tagatagcac tagaattcat ccagaagact accatttggc caccaaggtt gccgctgatg
     6901 ctttagaata cgatcctgat actattgccg aaaaagaaga acaggggact atgagtgaat
     6961 tcattgaact gttgagagaa gatcctgacc gtagagctaa actagaatca ctaaatctag
     7021 aatcatacgc agaagaactt gagaagaata ccggattaag aaaacttaat aatctaaata
     7081 caattgtcct tgaattgttg gatggatttg aagaattgag aaatgacttt catcctttgc
     7141 aaggtgatga aattttccaa agtttgactg gtgagtctga aaagacgttt ttcaagggta
     7201 gtattattcc agtcagagta gaaagattct ggcacaacga tataatttgc actacaaact
     7261 ctgaagttga atgtgtagta aatgctcaac gtcacgcagg tgcacaatta agaagacctg
     7321 caaatgaaat atacgaaatt ggtaaaacat atccagcaaa ggtgatatat attgactatg
     7381 ctaatattac tgcagaagtt tccttattag atcatgatgt caaacagcaa tatgttccaa
     7441 taagctacag taaagatcct tccatttggg acttgaaaca agaactggaa gatgccgaag
     7501 aggagaggaa attgatgatg gcagaagccc gtgcaaagag aacacatcgt gttatcaatc
     7561 atccttacta tttccctttc aacggcagac aggctgagga ttacttaagg agtaaagaac
     7621 gtggtgaatt cgtgatcaga cagtctagcc gaggtgatga ccacttggtt atcacctgga
     7681 aattggataa ggatttgttt caacatattg atatccaaga attagaaaaa gaaaatcctt
     7741 tggctttagg taaagtcttg attgtcgaca atcagaaata caatgattta gaccagatca
     7801 ttgtagaata tcttcaaaac aaggtaaggc tcttgaatga aatgacatct agtgaaaaat
     7861 tcaaaagcgg tactaagaaa gatgtggtca agtttattga agactactct agagtgaatc
     7921 caaataagtc tgtgtactat ttcagtttga accacgataa ccctggttgg ttttacttga
     7981 tgttcaagat taacgcaaat agcaaattat acacatggaa tgtgaaatta acgaacactg
     8041 gttatttcct ggtaaactac aattatccaa gtgttatcca gctttgtaat ggttttaaga
     8101 cgcttctaaa atctaacagt agtaagaata gaatgaacaa ctaccgttag atgcgtatgt
     8161 agtgtccatt gttattatta attttattat tactttgacc attgtttagg taaggtatgt
     8221 aaactagaac gcttcaactg tattttttac gggggatcca ctagttctag agcggccgcc
     8281 accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc gcttggcgta
     8341 atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat
     8401 aggagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt
     8461 aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc agctgcatta
     8521 atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc
     8581 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     8641 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     8701 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     8761 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     8821 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     8881 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     8941 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     9001 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     9061 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     9121 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     9181 cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     9241 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     9301 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     9361 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     9421 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     9481 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     9541 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     9601 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     9661 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     9721 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     9781 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     9841 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     9901 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     9961 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
    10021 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
    10081 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
    10141 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
    10201 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
    10261 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
    10321 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
    10381 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
    10441 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctgg
    10501 gtccttttca tcacgtgcta taaaaataat tataatttaa attttttaat ataaatatat
    10561 aaattaaaaa tagaaagtaa aaaaagaaat taaagaaaaa atagtttttg ttttccgaag
    10621 atgtaaaaga ctctaggggg atcgccaaca aatactacct tttatcttgc tcttcctgct
    10681 ctcaggtatt aatgccgaat tgtttcatct tgtctgtgta gaagaccaca cacgaaaatc
    10741 ctgtgatttt acattttact tatcgttaat cgaatgtata tctatttaat ctgcttttct
    10801 tgtctaataa atatatatgt aaagtacgct ttttgttgaa attttttaaa cctttgttta
    10861 tttttttttc ttcattccgt aactcttcta ccttctttat ttactttcta aaatccaaat
    10921 acaaaacata aaaataaata aacacagagt aaattcccaa attattccat cattaaaaga
    10981 tacgaggcgc gtgtaagtta caggcaagcg atccgtccta agaaaccatt attatcatga
    11041 cattaaccta taaaaatagg cgtatcacga ggccctttcg tc
//
