LOCUS       sequence-68284-e         941 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  lentiviral expression of myr-HA-Akt1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 941)
  AUTHORS   Cheng Z, Gong Y, Ma Y, Lu K, Lu X, Pierce LA, Thompson RC, Muller S,
            Knapp S, Wang J
  TITLE     Inhibition of BET bromodomain targets genetically diverse 
            glioblastoma.
  JOURNAL   Clin Cancer Res. 2013 Apr 1;19(7):1748-59. doi: 
            10.1158/1078-0432.CCR-12-3066. Epub 2013 Feb 12.
  PUBMED    23403638
REFERENCE   2  (bases 1 to 941)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1158/1078-0432.CCR-12-3066"; journalName: "Clin Cancer Res"; 
            date: "2013-04-1- 1"; volume: "19"; issue: "7"; pages: "1748-59"
COMMENT     Sequence Label: pCDH-puro-myr-HA-Akt1
FEATURES             Location/Qualifiers
     source          1..941
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     5..29
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     79..98
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        79..97
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             120..161
                     /codon_start=1
                     /product="N-myristoylation signal from Src kinase (Pellman 
                     et al., 1985; Kaplan et al., 1988)"
                     /label=myr
                     /translation="MGSSKSKPKDPSQR"
     CDS             165..191
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YAYDVPDYA"
     CDS             171..197
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
ORIGIN
        1 gcagagctcg tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc
       61 tccatagaag attctagata atacgactca ctatagggag acccaagctt cccaccacca
      121 tggggagcag caagagcaag cccaaggacc ccagccagcg cgcctacgcc tacgacgtgc
      181 ccgactacgc ctccctcgga tccatgagcg acgtggctat tgtgaaggag ggttggctgc
      241 acaaacgagg ggagtacatc aagacctggc ggccacgcta cttcctcctc aagaatgatg
      301 gcaccttcat tggctacaag gagcggccgc aggatgtgga ccaacgtgag gctcccctca
      361 acaacttctc tgtggcgcag tgccagctga tgaagacgga gcggccccgg cccaacacct
      421 tcatcatccg ctgcctgcag tggaccactg tcatcgaacg caccttccat gtggagactc
      481 ctgaggagcg ggaggagtgg acaaccgcca tccagactgt ggctgacggc ctcaagaagc
      541 aggaggagga ggagatggac ttccggtcgg gctcacccag tgacaactca ggggctgaag
      601 agatggaggt gtccctggcc aagcccaagc accgcgtgac catgaacgag tttgagtacc
      661 tgaagctgct gggcaagggc actttcggca aggtgatcct ggtgaaggag aaggccacag
      721 gccgctacta cgccatgaag atcctcaaga aggaagtcat cgtggccaag gacgaggtgg
      781 cccacacact caccgagaac cgcgtcctgc agaactccag gcaccccttc ctcacagccc
      841 tgaagtactc tttccagacc cacgaccgcc tctgctttgt catggagtac gccaacgggg
      901 gcgagctgtt cttccacctg tcccgggaac gtgtgttctc c
//
