LOCUS       sequence-68287-e         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin SH3 domain A
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(283..305)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(450..468)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        536..640
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(859..878)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccggcg atgtgaaggt tgtgtattac cgagcactct
       61 acccttttga tgcccgaagt catgatgaaa tcactattga gcctggagac attatcatgg
      121 tggatgaaag ccaaaccgga gagcctgggt ggctgggagg tgaactgaaa ggaaaaacgg
      181 gctggtttcc tgccaactat gcagagcgaa tgcccgagag tgaagaattc atcgtgactg
      241 actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct
      301 cccggagacg gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg
      361 cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag
      421 cggagtgtat aattcttgaa gacgaaaggg cctcgtgata cgcctatttt tataggttaa
      481 tgtcatgata ataatggttt cttagacgtc aggtggcact tttcggggaa atgtgcgcgg
      541 aacccctatt tgtttatttt tctaaataca ttcaaatatg tatccgctca tgagacaata
      601 accctgataa atgcttcaat aatattgaaa aaggaagagt atgagtattc aacatttccg
      661 tgtcgccctt attccctttt ttgcggcatt ttgccttcct gtttttgctc acccagaaac
      721 gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca cgagtgggtt acatcgaact
      781 ggatctcaac agcggtaaga tccttgagag ttttcgcccc gaagaacgtt ttccaatgat
      841 gagcactttt aaagttctgc tatgtggcgc ggtattatcc cgtgt
//
