LOCUS       sequence-68290-e         894 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 894)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 894)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin SH3 domain B
FEATURES             Location/Qualifiers
     source          1..894
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(325..347)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(492..510)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        578..682
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 aatcggatct ggttccgcgt ggatctcgtc gtgcatctgt tggatcctct cctgtacttc
       61 gtcagggtga aaaagtagaa ggccttcagg cacaagcctt gtatccttgg agagcaaaga
      121 aggacaacca tcttaatttt aacaaaaatg atgttatcac ggttcttgaa cagcaggata
      181 tgtggtggtt tggggaagtt caaggtcaaa agggctggtt tcccaaatcc tatgtaaagc
      241 ttatatccgg tccactaagg aaatctgaat tcatcgtgac tgactgacga tctgcctcgc
      301 gcgtttcggt gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc
      361 ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg
      421 cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat agcggagtgt ataattcttg
      481 aagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga taataatggt
      541 ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      601 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      661 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      721 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      781 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      841 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaa
//
