LOCUS       sequence-68291-e         846 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 846)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 846)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin SH3 domain C
FEATURES             Location/Qualifiers
     source          1..846
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             7..24
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(326..348)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(493..511)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        579..683
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 tcggatctgg ttccgcgtgg atctcgtcgt gcatctgttg gatccccagc atttaaacct
       61 gccatccaag gagaagaata catatccatg tacacatatg agagcaacga gcaaggagac
      121 ttgacctttc agcaaggaga tctgattgtg gtgattaaaa aagatggcga ctggtggact
      181 ggcacggtgg gagagaagac tggggttttc ccttccaact acgtgaggcc caaagactcg
      241 gaggcggctg gaagcggagg aaagacagaa ttcatcgtga ctgactgacg atctgcctcg
      301 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
      361 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
      421 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt
      481 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
      541 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
      601 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
      661 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
      721 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
      781 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
      841 agatcc
//
