LOCUS       sequence-68298-e         891 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 891)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 891)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin SH3 domain E
FEATURES             Location/Qualifiers
     source          1..891
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(277..299)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(444..462)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        530..634
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(853..872)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatcctcat tgcctcctac atgccaagtt atcgggatgt
       61 acgattatat cgcgcagaat gacgacgagt tggccttcag taaagggcag gtgattaacg
      121 tgctgaacaa agaggatccc gactggtgga aaggagaact gaatggccat gttggtctgt
      181 ttccctccaa ctacgtgaaa ctgaccacag acatggatga attcatcgtg actgactgac
      241 gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
      301 gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
      361 agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
      421 gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
      481 gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
      541 tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
      601 ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
      661 ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
      721 gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
      781 caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
      841 ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc a
//
