LOCUS       sequence-68299-e         960 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express a version of Fun30 without CUE domain in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 960)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 960)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA5
FEATURES             Location/Qualifiers
     source          1..960
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     17..36
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        17..35
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    88..99
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 ggactactag cagctgtaat acgactcact atagggaata ttaagctcgc ccttgtcgac
       61 gaaaacatga gtggttcgca ttcaaatgat gaggatgacg tagtgcaagt gcccgagacg
      121 tcctctccca ccaaggtagc atcgtcgtct cccttaaagc ctacttcgcc aacagttccg
      181 gatgcaagtg tggcgtcttt gagaagcagg tttactttca agccttcaga tcccagcgaa
      241 ggagctcata cttcgaagcc gctcccatct gggagtcctg aggataggca gcaaagacaa
      301 aattggacat ggaacaagaa cgcatctccc aagaagtcag aaaccccgcc acctgtcaag
      361 aagtcattac cactggcaaa cacaggccgt ttatcatcta tccatggcaa tatcaacaac
      421 aaatcctcca agattaccgt ggccaaacag aaaacgtcca tttttgaccg ttactcaaac
      481 gtcatcaacc agaaacaata cacttttgag ctgccaacta acttgaatat agactcggag
      541 gcactgagca agttgcccgt gaactacaac aaaaagagaa ggctggtaag ggcagatcag
      601 catccaattg gcaagtctta tgagtcatcc gctacacaat taggttctgc aagagagaaa
      661 ctactggcga accgcaaata cggtcgtcat gcaaacgaca acgatgaaga ggaggaagag
      721 agtatgatga cggacgatga cgatgcaagt ggcgacgact acacagaatc cacgccgcag
      781 ataaatctgg atgaacaagt tttacagttt attaatgact ctgatattgt cgatctctcg
      841 gacctctcag ataccacgat gcataaggct caactcatag cctcacatag gccatattct
      901 tctttaaatg cctttgtaaa cacaaatttc aatgataagg acactgagga gaacgcatcg
//
