LOCUS       sequence-68301-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Micheva KD, Kay BK, McPherson PS
  TITLE     Synaptojanin forms two separate complexes in the nerve terminal. 
            Interactions with endophilin and amphiphysin.
  JOURNAL   J Biol Chem. 1997 Oct 24;272(43):27239-45.
  PUBMED    9341169
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1997-10-24"; volume: "272(43)"; pages: "27239-45"
COMMENT     Sequence Label: Endophilin SH3 domains
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(295..317)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(462..480)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        548..652
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(871..890)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatctcgtc gtgcatctgt tggatccgat cagccctgct
       61 gccgagctct gtatgacttt gaacctgaaa atgaagggga attgggtttt aaagagggcg
      121 atatcatcac actcactaat cagattgacg agaactggta tgaggggatg cttcatggcc
      181 agtctggctt tttccccatc aactatgtag aaattctggt tgctctgccc cattgagaat
      241 tcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg aaaacctctg
      301 acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca
      361 agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc
      421 acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga tacgcctatt
      481 tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg
      541 aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct
      601 catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat
      661 tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc
      721 tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg
      781 ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg
      841 ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtgttga
      901 cgccgggcaa gagcaactcg gtcgccgcat acacta
//
