LOCUS       sequence-68303-e         916 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express a version of Fun30 without CUE domain in budding yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 916)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 916)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA5
FEATURES             Location/Qualifiers
     source          1..916
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(12..29)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(39..80)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 ctagaaactc aatggtgatg gtgatgatga ccggtacgcg tagaatcgag accgaggaga
       61 gggttaggga taggcttacc ttcgaatggg tgacctcgaa gctcgccctt ctcgagttct
      121 ttggttccct tcggtttcga gttttcatca taaattatat cctccaacat atcactaact
      181 ttactttcca acacatcttg agatttttta tcttcactga tatacgaatc taaagctagt
      241 ttatttttgg ccagttgatg aatcttttcc tctatggaat ccttagtaat taaagtggtt
      301 atattaactt cctttgtttg tcccacacga tgtgccctat cagcagcttg tctgtcatca
      361 tgtgggttaa aactttgatc gaatataata acattatttg cgcacaccaa attaataccg
      421 aatccacctg cctttgttga taagatgaaa atgggaatat ccttatcttc ataaaactta
      481 tctattagta gttgtctatc attcacttgc gtggaaccat ctaatcttaa aaatttataa
      541 tctaaggtgg acaaaaccat ctctagaata tccaggactt gagtgaataa ggaaaatatc
      601 agcacctttt cctgtttgtc aacaatgatt gttttcagca attttttcaa agcgtctatc
      661 ttcccagatt gcatccactc gtcattatga agttggtatt tggataacgt gttcgggaaa
      721 ttgcagcata gtttgtgcaa ctcaaaatcc gtcatatagc tcatatcttc cttaatatac
      781 tctttgttac cgttttcagc ataagcaggt tcatccaata tggcatcact cattttagtg
      841 atgattttat cattatagat atttctgaac aaaagtggat gcagagaggc ctttcgcaat
      901 gccattatta aatttt
//
