LOCUS       sequence-68304-e         966 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express a version of Fun30 with a mutated CUE domain in budding 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 966)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 966)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA12
FEATURES             Location/Qualifiers
     source          1..966
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     20..39
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        20..38
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    91..102
                     /label=ZF binding site
                     /bound_moiety="CCR5TC zinc-finger domain"
                     /note="target sequence for the CCR5TC zinc-finger domain 
                     from the CCR5-224 (+) zinc-finger nuclease (Perez et al., 
                     2008; Gross et al., 2013)"
ORIGIN
        1 atcggactac tagcagctgt aatacgactc actataggga atattaagct cgcccttgtc
       61 gacgaaaaca tgagtggttc gcattcaaat gatgaggatg acgtagtgca agtgcccgag
      121 acgtcctctc ccaccaaggt agcatcgtcg tctcccttaa agcctacttc gccaacagtt
      181 ccggatgcaa gtgtggcgtc tttgagaagc aggtttactt tcaagccttc agatcccagc
      241 gaaggagctc atacttcgaa gccgctccca tctgggagtc ctgaggtagc actggttaac
      301 cttgcgagag aggccgccga tttctctcaa actctggtgc aggctgtttt caaatctaac
      361 tcttttaact tacagtctgc cagggaacgt cttacaagat tgaggcagca aagacaaaat
      421 tggacatgga acaagaacgc atctcccaag aagtcagaaa ccccgccacc tgtcaagaag
      481 tcattaccac tggcaaacac aggccgttta tcatctatcc atggcaatat caacaacaaa
      541 tcctccaaga ttaccgtggc caaacagaaa acgtccattt ttgaccgtta ctcaaacgtc
      601 atcaaccaga aacaatacac ttttgagctg ccaactaact tgaatataga ctcggaggca
      661 ctgagcaagt tgcccgtgaa ctacaacaaa aagagaaggc tggtaagggc agatcagcat
      721 ccaattggca agtcttatga gtcatccgct acacaattag gttctgcaag agagaaacta
      781 ctggcgaacc gcaaatacgg tcgtcatgca aacgacaacg atgaagagga ggaagagagt
      841 atgatgacgg acgatgacga tgcaagtggc gacgactaca cagaatccac gccgcagata
      901 aatctggatg aacaagtttt acagtttatt aatgactctg atattgtcga tctctcggac
      961 ctctca
//
