LOCUS       sequence-68307-e        6243 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  PesaR promoter, luxCDABE, and terminator in pET17b (AmpR).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 6243)
  AUTHORS   Shong J, Collins CH
  TITLE     Engineering the esaR Promoter for Tunable Quorum Sensing-Dependent 
            Gene Expression.
  JOURNAL   ACS Synth Biol. 2013 Jul 23.
  PUBMED    23879176
REFERENCE   2  (bases 1 to 6243)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "ACS Synth 
            Biol. 2013 Jul 23."
COMMENT     Sequence Label: pET-PesaRlux
FEATURES             Location/Qualifiers
     source          1..6243
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             318..1760
                     /codon_start=1
                     /gene="luxC"
                     /product="LuxC fatty acid reductase"
                     /label=LuxC
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTKKISFIINGQVEIFPESDDLVQSINFGDNSVYLPILNDSHVKN
                     IIDCNGNNELRLHNIVNFLYTVGQRWKNEEYSRRRTYIRDLKKYMGYSEEMAKLEANWI
                     SMILCSKGGLYDVVENELGSRHIMDEWLPQDESYVRAFPKGKSVHLLAGNVPLSGIMSI
                     LRAILTKNQCIIKTSSTDPFTANALALSFIDVDPNHPITRSLSVIYWPHQGDTSLAKEI
                     MRHADVIVAWGGPDAINWAVEHAPSYADVIKFGSKKSLCIIDNPVDLTSAATGAAHDVC
                     FYDQRACFSAQNIYYMGNHYEEFKLALIEKLNLYAHILPNAKKDFDEKAAYSLVQKESL
                     FAGLKVEVDIHQRWMIIESNAGVEFNQPLGRCVYLHHVDNIEQILPYVQKNKTQTISIF
                     PWESSFKYRDALALKGAERIVEAGMNNIFRVGGSHDGMRPLQRLVTYISHERPSNYTAK
                     DVAVEIEQTRFLEEDKFLVFVP"
     CDS             1772..2695
                     /codon_start=1
                     /gene="luxD"
                     /product="LuxD acyltransferase"
                     /label=LuxD
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MENESKYKTIDHVICVEGNKKIHVWETLPEENSPKRKNAIIIASG
                     FARRMDHFAGLAEYLSRNGFHVIRYDSLHHVGLSSGTIDEFTMSIGKQSLLAVVDWLTT
                     RKINNFGMLASSLSARIAYASLSEINASFLITAVGVVNLRYSLERALGFDYLSLPINEL
                     PDNLDFEGHKLGAEVFARDCLDFGWEDLASTINNMMYLDIPFIAFTANNDNWVKQDEVI
                     TLLSNIRSNRCKIYSLLGSSHDLSENLVVLRNFYQSVTKAAIAMDNDHLDIDVDITEPS
                     FEHLTIATVNERRMRIEIENQAISLS"
     CDS             2744..3826
                     /codon_start=1
                     /gene="luxA"
                     /product="LuxA luciferase subunit"
                     /label=LuxA
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGNFLLTYQPPQFSQTEVMKRLVKLGRISEECGFDTVWLLEHH
                     FTEFGLLGNPYVAAAYLLGATKKLNVGTAAIVLPTAHPVRQLEDVNLLDQMSKGRFRFG
                     ICRGLYNKDFRVFGTDMNNSRALAECWYGLIKNGMTEGYMEADNEHIKFHKVKVNPAAY
                     SRGGAPVYVVAESASTTEWAAQFGLPMILSWIINTNEKKAQLELYNEVAQEYGHDIHNI
                     DHCLSYITSVDHDSIKAKEICRKFLGHWYDSYVNATTIFDDSDQTRGYDFNKGQWRDFV
                     LKGHKDTNRRIDYSYEINPVGTPQECIDIIQKDIDATGISNICCGFEANGTVDEIIASM
                     KLFQSDVMPFLKEKQRSLLY"
     CDS             3841..4824
                     /codon_start=1
                     /gene="luxB"
                     /product="LuxB luciferase subunit"
                     /label=LuxB
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MKFGLFFLNFINSTTVQEQSIVRMQEITEYVDKLNFEQILVYENH
                     FSDNGVVGAPLTVSGFLLGLTEKIKIGSLNHIITTHHPVAIAEEACLLDQLSEGRFILG
                     FSDCEKKDEMHFFNRPVEYQQQLFEECYEIINDALTTGYCNPDNDFYSFPKISVNPHAY
                     TPGGPRKYVTATSHHIVEWAAKKGIPLIFKWDDSNDVRYEYAERYKAVADKYDVDLSEI
                     DHQLMILVNYNEDSNKAKQETRAFISDYVLEMHPNENFENKLEEIIAENAVGNYTECIT
                     AAKLAIEKCGAKSVLLSFEPMNDLMSQKNVINIVDDNIKKYHMEYT"
     CDS             5003..6115
                     /codon_start=1
                     /gene="luxE"
                     /product="LuxE"
                     /label=LuxE
                     /note="from the Photorhabdus luminescens bioluminescence 
                     operon"
                     /translation="MTSYVDKQEITASSEIDDLIFSSDPLVWSYDEQEKIRKKLVLDAF
                     RNHYKHCREYRHYCQAHKVDDNITEIDDIPVFPTSVFKFTRLLTSQENEIESWFTSSGT
                     NGLKSQVARDRLSIERLLGSVSYGMKYVGSWFDHQIELVNLGPDRFNAHNIWFKYVMSL
                     VELLYPTTFTVTEERIDFVKTLNSLERIKNQGKDLCLIGSPYFIYLLCHYMKDKKISFS
                     GDKSLYIITGGGWKSYEKESLKRDDFNHLLFDTFNLSDISQIRDIFNQVELNTCFFEDE
                     MQRKHVPPWVYARALDPETLKPVPDGTPGLMSYMDASATSYPAFIVTDDVGIISREYGK
                     YPGVLVEILRRVNTRTQKGCALSLTEAFDS"
     terminator      6148..6234
                     /gene="Escherichia coli rrnB"
                     /label=rrnB T1 terminator
                     /note="transcription terminator T1 from the E. coli rrnB 
                     gene"
ORIGIN
        1 ctcgaggcag attgagtaac cgtgaatgtt tgtacaaatg tttcaaagat gttactatga
       61 gtgtcccggc cagcatcact ttatattttg tgacgtctgg ccggacgttt tccctagtgt
      121 tggctgtttt agcgacctgg ccgtacaggt caggtttttt tttaccgcta aacaactgaa
      181 gccattgtaa cctctgaatg attcattgta agttactctt aagtatcatc ttgcctgtac
      241 tatagtgcag gttaagtcca cgttaagtaa aagaagcagc ggatcctcta gttgcggccg
      301 caaaatggat ggcaaatatg actaaaaaaa tttcattcat tattaacggc caggttgaaa
      361 tctttcccga aagtgatgat ttagtgcaat ccattaattt tggtgataat agtgtttacc
      421 tgccaatatt gaatgactct catgtaaaaa acattattga ttgtaatgga aataacgaat
      481 tacggttgca taacattgtc aattttctct atacggtagg gcaaagatgg aaaaatgaag
      541 aatactcaag acgcaggaca tacattcgtg acttaaaaaa atatatggga tattcagaag
      601 aaatggctaa gctagaggcc aattggatat ctatgatttt atgttctaaa ggcggccttt
      661 atgatgttgt agaaaatgaa cttggttctc gccatatcat ggatgaatgg ctacctcagg
      721 atgaaagtta tgttcgggct tttccgaaag gtaaatctgt acatctgttg gcaggtaatg
      781 ttccattatc tgggatcatg tctatattac gcgcaatttt aactaagaat cagtgtatta
      841 taaaaacatc gtcaaccgat ccttttaccg ctaatgcatt agcgttaagt tttattgatg
      901 tagaccctaa tcatccgata acgcgctctt tatctgttat atattggccc caccaaggtg
      961 atacatcact cgcaaaagaa attatgcgac atgcggatgt tattgtcgct tggggagggc
     1021 cagatgcgat taattgggcg gtagagcatg cgccatctta tgctgatgtg attaaatttg
     1081 gttctaaaaa gagtctttgc attatcgata atcctgttga tttgacgtcc gcagcgacag
     1141 gtgcggctca tgatgtttgt ttttacgatc agcgagcttg tttttctgcc caaaacatat
     1201 attacatggg aaatcattat gaggaattta agttagcgtt gatagaaaaa cttaatctat
     1261 atgcgcatat attaccgaat gccaaaaaag attttgatga aaaggcggcc tattctttag
     1321 ttcaaaaaga aagcttgttt gctggattaa aagtagaggt ggatattcat caacgttgga
     1381 tgattattga gtcaaatgca ggtgtggaat ttaatcaacc acttggcaga tgtgtgtacc
     1441 ttcatcacgt cgataatatt gagcaaatat tgccttatgt tcaaaaaaat aagacgcaaa
     1501 ccatatctat ttttccttgg gagtcatcat ttaaatatcg agatgcgtta gcattaaaag
     1561 gtgcggaaag gattgtagaa gcaggaatga ataacatatt tcgagttggt ggatctcatg
     1621 acggaatgag accgttgcaa cgattagtga catatatttc tcatgaaagg ccatctaact
     1681 atacggctaa ggatgttgcg gttgaaatag aacagactcg attcctggaa gaagataagt
     1741 tccttgtatt tgtcccataa taggtaaaag tatggaaaat gaatcaaaat ataaaaccat
     1801 cgaccacgtt atttgtgttg aaggaaataa aaaaattcat gtttgggaaa cgctgccaga
     1861 agaaaacagc ccaaagagaa agaatgccat tattattgcg tctggttttg cccgcaggat
     1921 ggatcatttt gctggtctgg cggaatattt atcgcggaat ggatttcatg tgatccgcta
     1981 tgattcgctt caccacgttg gattgagttc agggacaatt gatgaattta caatgtctat
     2041 aggaaagcag agcttgttag cagtggttga ttggttaact acacgaaaaa taaataactt
     2101 cggtatgttg gcttcaagct tatctgcgcg gatagcttat gcaagcctat ctgaaatcaa
     2161 tgcttcgttt ttaatcaccg cagtcggtgt tgttaactta agatattctc ttgaaagagc
     2221 tttagggttt gattatctca gtctacccat taatgaattg ccggataatc tagattttga
     2281 aggccataaa ttgggtgctg aagtctttgc gagagattgt cttgattttg gttgggaaga
     2341 tttagcttct acaattaata acatgatgta tcttgatata ccgtttattg cttttactgc
     2401 aaataacgat aattgggtca agcaagatga agttatcaca ttgttatcaa atattcgtag
     2461 taatcgatgc aagatatatt ctttgttagg aagttcgcat gacttgagtg aaaatttagt
     2521 ggtcctgcgc aatttttatc aatcggttac gaaagccgct atcgcgatgg ataatgatca
     2581 tctggatatt gatgttgata ttactgaacc gtcatttgaa catttaacta ttgcgacagt
     2641 caatgaacgc cgaatgagaa ttgagattga aaatcaagca atttctctgt cttaaaatct
     2701 attgagatat tctatcactc aaatagcaat ataaggactc tctatgaaat ttggaaactt
     2761 tttgcttaca taccaacctc cccaattttc tcaaacagag gtaatgaaac gtttggttaa
     2821 attaggtcgc atctctgagg agtgtggttt tgataccgta tggttactgg agcatcattt
     2881 cacggagttt ggtttgcttg gtaaccctta tgtcgctgct gcatatttac ttggcgcgac
     2941 taaaaaattg aatgtaggaa ctgccgctat tgttcttccc acagcccatc cagtacgcca
     3001 acttgaagat gtgaatttat tggatcaaat gtcaaaagga cgatttcggt ttggtatttg
     3061 ccgagggctt tacaacaagg actttcgcgt attcggcaca gatatgaata acagtcgcgc
     3121 cttagcggaa tgctggtacg ggctgataaa gaatggcatg acagagggat atatggaagc
     3181 tgataatgaa catatcaagt tccataaggt aaaagtaaac cccgcggcgt atagcagagg
     3241 tggcgcaccg gtttatgtgg tggctgaatc agcttcgacg actgagtggg ctgctcaatt
     3301 tggcctaccg atgatattaa gttggattat aaatactaac gaaaagaaag cacaacttga
     3361 gctttataat gaagtggctc aagaatatgg gcacgatatt cataatatcg accattgctt
     3421 atcatatata acatctgtag atcatgactc aattaaagcg aaagagattt gccggaaatt
     3481 tctggggcat tggtatgatt cttatgtgaa tgctacgact atttttgatg attcagacca
     3541 aacaagaggt tatgatttca ataaagggca gtggcgtgac tttgtattaa aaggacataa
     3601 agatactaat cgccgtattg attacagtta cgaaatcaat cccgtgggaa cgccgcagga
     3661 atgtattgac ataattcaaa aagacattga tgctacagga atatcaaata tttgttgtgg
     3721 atttgaagct aatggaacag tagacgaaat tattgcttcc atgaagctct tccagtctga
     3781 tgtcatgcca tttcttaaag aaaaacaacg ttcgctatta tattagctaa ggagaaagaa
     3841 atgaaatttg gattgttctt ccttaacttc atcaattcaa caactgttca agaacaaagt
     3901 atagttcgca tgcaggaaat aacggagtat gttgataagt tgaattttga acagatttta
     3961 gtgtatgaaa atcatttttc agataatggt gttgtcggcg ctcctctgac tgtttctggt
     4021 tttctgctcg gtttaacaga gaaaattaaa attggttcat taaatcacat cattacaact
     4081 catcatcctg tcgccatagc ggaggaagct tgcttattgg atcagttaag tgaagggaga
     4141 tttattttag ggtttagtga ttgcgaaaaa aaagatgaaa tgcatttttt taatcgcccg
     4201 gttgaatatc aacagcaact atttgaagag tgttatgaaa tcattaacga tgctttaaca
     4261 acaggctatt gtaatccaga taacgatttt tatagcttcc ctaaaatatc tgtaaatccc
     4321 catgcttata cgccaggcgg acctcggaaa tatgtaacag caaccagtca tcatattgtt
     4381 gagtgggcgg ccaaaaaagg tattcctctc atctttaagt gggatgattc taatgatgtt
     4441 agatatgaat atgctgaaag atataaagcc gttgcggata aatatgacgt tgacctatca
     4501 gagatagacc atcagttaat gatattagtt aactataacg aagatagtaa taaagctaaa
     4561 caagagacgc gtgcatttat tagtgattat gttcttgaaa tgcaccctaa tgaaaatttc
     4621 gaaaataaac ttgaagaaat aattgcagaa aacgctgtcg gaaattatac ggagtgtata
     4681 actgcggcta agttggcaat tgaaaagtgt ggtgcgaaaa gtgtattgct gtcctttgaa
     4741 ccaatgaatg atttgatgag ccaaaaaaat gtaatcaata ttgttgatga taatattaag
     4801 aagtaccaca tggaatatac ctaatagatt tcgagttgca gcgaggcggc aagtgaacga
     4861 atccccagga gcatagataa ctatgtgact ggggtgagtg aaagcagcca acaaagcagc
     4921 agcttgaaag atgaagggta taaaagagta tgacagcagt gctgccatac tttctaatat
     4981 tatcttgagg agtaaaacag gtatgacttc atatgttgat aaacaagaaa ttacagcaag
     5041 ctcagaaatt gatgatttga ttttttcgag cgatccatta gtgtggtctt acgacgagca
     5101 ggaaaaaatc agaaagaaac ttgtgcttga tgcatttcgt aatcattata aacattgtcg
     5161 agaatatcgt cactactgtc aggcacacaa agtagatgac aatattacgg aaattgatga
     5221 catacctgta ttcccaacat cggtttttaa gtttactcgc ttattaactt ctcaggaaaa
     5281 cgagattgaa agttggttta ccagtagcgg cacgaatggt ttaaaaagtc aggtggcgcg
     5341 tgacagatta agtattgaga gactcttagg ctctgtgagt tatggcatga aatatgttgg
     5401 tagttggttt gatcatcaaa tagaattagt caatttggga ccagatagat ttaatgctca
     5461 taatatttgg tttaaatatg ttatgagttt ggtggaattg ttatatccta cgacatttac
     5521 cgtaacagaa gaacgaatag attttgttaa aacattgaat agtcttgaac gaataaaaaa
     5581 tcaagggaaa gatctttgtc ttattggttc gccatacttt atttatttac tctgccatta
     5641 tatgaaagat aaaaaaatct cattttctgg agataaaagc ctttatatca taaccggagg
     5701 cggctggaaa agttacgaaa aagaatctct gaaacgtgat gatttcaatc atcttttatt
     5761 tgatactttc aatctcagtg atattagtca gatccgagat atatttaatc aagttgaact
     5821 caacacttgt ttctttgagg atgaaatgca gcgtaaacat gttccgccgt gggtatatgc
     5881 gcgagcgctt gatcctgaaa cgttgaaacc tgtacctgat ggaacgccgg ggttgatgag
     5941 ttatatggat gcgtcagcaa ccagttatcc agcatttatt gttaccgatg atgtcgggat
     6001 aattagcaga gaatatggta agtatcccgg cgtgctcgtt gaaattttac gtcgcgtcaa
     6061 tacgaggacg cagaaagggt gtgctttaag cttaaccgaa gcgtttgata gttgatatcc
     6121 tttgcctgcg gccgcaacta gaggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     6181 gcctttcgtt ttatctgttg tttgtcggtg aacgctctcc tgagtaggac aaatccgaga
     6241 tct
//
