LOCUS       sequence-68308-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  To express a version of Fun30 with a mutated CUE domain in budding 
            yeast.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Neves-Costa A, Will WR, Vetter AT, Miller JR, Varga-Weisz P
  TITLE     The SNF2-family member Fun30 promotes gene silencing in 
            heterochromatic loci.
  JOURNAL   PLoS One. 2009 Dec 1;4(12):e8111. doi: 10.1371/journal.pone.0008111.
  PUBMED    19956593
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "PLoS One.";
            date: "2009-12-1"; volume: "4(12):e8111. doi"; pages: " 
            10.1371/journal.pone.0008111"
COMMENT     Sequence Label: pFA12
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(8..25)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             complement(35..76)
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
ORIGIN
        1 aaactcaatg gtgatggtga tgatgaccgg tacgcgtaga atcgagaccg aggagagggt
       61 tagggatagg cttaccttcg aatgggtgac ctcgaagctc gcccttctcg agttctttgg
      121 ttcccttcgg tttcgagttt tcatcataaa ttatatcctc caacatatca ctaactttac
      181 tttccaacac atcttgagat tttttatctt cactgatata cgaatctaaa gctagtttat
      241 ttttggccag ttgatgaatc ttttcctcta tggaatcctt agtaattaaa gtggttatat
      301 taacttcctt tgtttgtccc acacgatgtg ccctatcagc agcttgtctg tcatcatgtg
      361 ggttaaaact ttgatcgaat ataataacat tatttgcgca caccaaatta ataccgaatc
      421 cacctgcctt tgttgataag atgaaaatgg gaatatcctt atcttcataa aacttatcta
      481 ttagtagttg tctatcattc acttgcgtgg aaccatctaa tcttaaaaat ttataatcta
      541 aggtggacaa aaccatctct agaatatcca ggacttgagt gaataaggaa aatatcagca
      601 ccttttcctg tttgtcaaca atgattgttt tcagcaattt tttcaaagcg tctatcttcc
      661 cagattgcat ccactcgtca ttatgaagtt ggtatttgga taacgtgttc gggaaattgc
      721 agcatagttt gtgcaactca aaatccgtca tatagctcat atcttcctta atatactctt
      781 tgttaccgtt ttcagcataa gcaggttcat ccaatatggc atcactcatt ttagtgatga
      841 ttttatcatt atagatattt ctgaacaaaa gtggatgcag agaggccttt cgcaatgcca
      901 ttattaaatt ttgga
//
