LOCUS       sequence-68310-e         856 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 856)
  AUTHORS   Ramjaun AR, Angers A, Legendre-Guillemin V, Tong XK, McPherson PS
  TITLE     Endophilin regulates JNK activation through its interaction with the
            germinal center kinase-like kinase.
  JOURNAL   J Biol Chem. 2001 Aug 3;276(31):28913-9. Epub 2001 May 30.
  PUBMED    11384986
REFERENCE   2  (bases 1 to 856)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "2001-08-3"; volume: "276(31)"; pages: "28913-9. Epub 
            2001 May 30"
COMMENT     Sequence Label: Flag-GLK
FEATURES             Location/Qualifiers
     source          1..856
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     47..66
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        47..65
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      79..88
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             88..111
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 ctctctggct aactagagaa cccactgctt actggcttat cgaaattaat acgactcact
       61 atagggagac ccaagcttgc caccatggac tacaaagacg atgacgacaa accgcaggag
      121 gacttcgagc tgatccaacg cataggcagc ggcacctacg gcgacgtcta caaggcacgg
      181 aatgtcaaca ctggagaatt agccgcaatt aaagtgataa aattggaacc aggagaagac
      241 ttcgcggttg tacagcaaga aatcattatg atgaaagact gcaaacatgc aaacatcgtt
      301 gcttattttg gaagttatct caggcgagat aaactttgga tttgcatgga attttgtgga
      361 ggtggctctt tacaggacat ctatcatgta actggccccc tttcagagct gcagattgca
      421 tacgttagca gggaaacact ccagggattg tattaccttc acagtaaagg aaaaatgcac
      481 agagatataa agggcgctaa catcctacta acggataacg gtcatgtgaa attagctgat
      541 tttggagttt ccgcacagat aacagctaca attgccaaac ggaagtcctt cattggtacc
      601 ccatattgga tggccccaga agtcgcggct gttgagcgga aaggagggta caatcagctg
      661 tgtgacctct gggctgtggg catcactgcc atcgagctgg cggagctcca gccacccatg
      721 tttgaccttc acccaatgag ggcattgttt ctaatgacaa aaagcaattt ccagcctcct
      781 aaattaaaag ataagttgaa gtggtcaaat agtttccatc attttgtgaa gatggctctt
      841 accaaaaatc caaaaa
//
