LOCUS       sequence-68315-e       11679 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP1 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11679)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 11679)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP1-bio
FEATURES             Location/Qualifiers
     source          1..11679
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    7059..7114
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(7113..7132)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(9350..9368)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        9436..9540
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(9541..9609,9610..10401)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(9759..9778)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      10572..11160
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     11061..11080
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     11314..11331
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    11448..11469
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(11484..11489,11490..11507,11508..11514)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    11522..11538
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     11527..11549
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     11546..11562
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     11546..11562
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccgtga cccacgagag ctaccaggaa ctggtcaaga
     1381 agctggaagc cctggaagat gccgtgctga ccggctacag cctgttccag aaagaaaaga
     1441 tggtgctgaa cgaggaagag atcaccacca agggcgcctc tgcccagagt ggagcctctg
     1501 ctcagtctgg ggcttctgcc cagtctggtg cctcagcaca gagcggcgct tcagcacagt
     1561 caggcgcaag cgcacagagc ggaacatctg gccctagcgg cccttctggc accagcccca
     1621 gcagcagaag caacaccctg ccccggtcta ataccgccag cggcgcctct cctcctgccg
     1681 acgccagcga tagcgacgcc aagagctacg ccgacctgaa gcacagagtg cggaactacc
     1741 tgttcaccat caaagagctg aagtaccccg agctgttcga cctgaccaac cacatgctga
     1801 ccctgtgcga caacatccac ggcttcaagt acctgatcga cggctacgag gaaatcaacg
     1861 agctgctgta caagctgaac ttctacttcg atctgctgcg ggccaagctg aacgacgtgt
     1921 gcgccaacga ctactgccag atccccttca acctgaagat ccgggccaac gagctggacg
     1981 tgctgaagaa actggtgttc ggctaccgga agcccctgga caacatcaag gacaacgtgg
     2041 gcaagatgga agattacatc aagaagaaca aggccacaat cgccaacatc aatgagctga
     2101 tcgagggcag caagaaaacc atcgaccaga acaagaacgc cgacaacgag gaaggcaaga
     2161 agaagctgta ccaggcccag tacgacctga gcatctacaa caagcagctg gaagaggccc
     2221 acaacctgat cagcgtgctg gaaaagcgga tcgacaccct gaagaagaac gagaacatca
     2281 agaaactgct ggacaagatc aacgagatca agaacccccc tcccgccaac agcggcaaca
     2341 cccccaatac cctgctggat aagaacaaga agatcgagga acacgaagag aagatcaaag
     2401 agattgccaa gaccatcaag ttcaacatcg actccctgtt caccgacccc ctggaactgg
     2461 aatactacct gagagagaag aacaaaaagg tcgacgtgac ccccaagagc caggacccca
     2521 ccaagagcgt gcagatcccc aaggtgccct accccaacgg catcgtgtac cccctgcccc
     2581 tgaccgacat ccacaacagc ctggccgccg acaatgacaa gaactcctac ggcgacctga
     2641 tgaaccccca caccaaagag aagattaacg agaagatcat caccgacaac aaagagcgga
     2701 agatcttcat caacaacatc aaaaagaaga ttgacctcga ggaaaagaac atcaaccacg
     2761 ccaaagagca gaataagaag ctgctcgagg actacgagaa gtccaagaag gattacgagg
     2821 aactgctgga aaaattctac gagatgaagt tcaacaacaa cttcaacaag gacgtggtgg
     2881 ataagatctt cagcgcccgg tacacctaca acgtggagaa gcagcggtac aacaacaagt
     2941 tcagcagcag caacaacgcc gtgtacaatg tgcagaagct gaagaaggcc ctgagctacc
     3001 tggaagatta tagcctgcgg aagggcatca gcgagaagga cttcaaccac tactacaccc
     3061 tgaaaaccgg cctggaagcc gacattaaga agctgaccga agagatcaag agcagcgaga
     3121 acaagatcct ggaaaagaac tttaagggcc tgacacacag cgccaacggc gccctggaag
     3181 tgtccgacat cgtgaagctc caggtgcaga aggtgctgct gatcaagaaa attgaggacc
     3241 tgagaaagat cgagctgttc ctgaagaacg cccagctgaa ggacagcatc cacgtgccca
     3301 acatctacaa gccccagaac aagcccgagc cctactacct gatcgtgctg aaaaaagagg
     3361 tggacaagct gaaagaattc atccccaaag tgaaggacat gctcaagaaa gaacaggccg
     3421 tgctgtccag catcacccag cctctggtgg ccgccagcga gacaacagag gacggcggcc
     3481 acagcacaca cacactgagc cagtccggcg agacagaagt gaccgaggaa accgaagaga
     3541 cagaggaaac agtgggccac accaccaccg tgaccatcac cctgcctccc acccagccta
     3601 gcccccccaa agaagtgaag gtcgtcgaga acagcatcga gcacaagagc aacgacaaca
     3661 gccaggccct gaccaagacc gtgtacctga aaaagctgga cgagttcctg accaagtcct
     3721 acatctgcca caagtacatc ctggtgtcca acagcgccat ggaccagaag ctgctcgaag
     3781 tctacaacct ggcccctgag gaagagaacg agctgaagtc ctgcgaccct ctggatctgc
     3841 tgttcaacat ccagaacaac atccccgcca tgtacagcct gtacgacagc atgaacaacg
     3901 atctgcagca cctcttcttc gagctgtatc agaaagagat gatctactat ctgcacaagc
     3961 tcaaagagga aaaccacatc aaaaaactcc tcgaagaaca gaagcagatc accggcacca
     4021 gcagcaccag ctccccaggc aacaccgccg tgaataccgc ccagagcgcc acccacagca
     4081 acagccagaa ccagcagagc aacgccgcca gcaccaacac acagaacggc gtggccgtgt
     4141 ctagtggccc tgccgtggtg gaggaaagcc acgaccccct gaccgtgctg tctatcagca
     4201 acgacctgaa gggcatcgtg tccctgctga acctgggcaa caaggccaag gtgccaaacc
     4261 ctctgaccat cagcaccacc gagatggaaa agttctatga gaacatcctg aagaataatg
     4321 acgcctactt caacgacgac atcaagcagt tcgtgaagtc caacagcaaa gtgatcaccg
     4381 gcctgaccga gacacagaag aatgccctga acgacgagat taagaaactg aaggacaccc
     4441 tgcagctgtc cttcgacctg tacaacaagt acaaactgaa gctggacaga ctgttcaaca
     4501 agaagaaaga gctgggccag gacaagatgc agatcaaaaa gctgaccctg ctcaaagagc
     4561 agctcgagag caagctgaac agcctgaaca acccccacaa cgtgctgcag aacttcgccg
     4621 tgttcttcaa taagaaaaaa gaagccgaga tcgccgagac agagaacacc ctggaaaaca
     4681 ccaagatcct gctgaagcac tacaagggcc tggtcaagta ctacaacggc gagagcagcc
     4741 ctctgaaaac cctgagcgag gtgtccatcc agaccgagga caactacgcc aacctggaaa
     4801 agtttcgggt gctgtccaag atcgacggca agctgaatga caatctgcac ctgggcaaga
     4861 aaaaactgag cttcctgagc agcggcctgc accacctgat caccgagctg aaagaggtca
     4921 tcaagaataa gaactacgcc ggcaacagcc cctccgagaa caataagaaa gtgaacgagg
     4981 ccctgaagtc ctacgagaac ttcctgcccg aggccaaagt cactaccgtg gtcacccccc
     5041 ctcagcctga tgtgaccccc agccccctgt ccgtgagagt gtctggcagc agcggcagca
     5101 ccaaagaaga gacacagatc cccacctccg gcagcctgct gacagagctg cagcaagtcg
     5161 tgcagctgca gaattacgac gaagaggacg acagcctggt ggtgctgccc atcttcggcg
     5221 agagcgagga caacgacgag tacctggacc aggtggtcac aggcgaggcc atcagcgtga
     5281 ccatggacaa tatcctgagc ggcttcgaga acgagtacga cgtgatctac ctgaagcctc
     5341 tggccggcgt gtaccgcagc ctgaagaagc agattgagaa gaacatcttc accttcaatc
     5401 tgaacctgaa cgacatcctg aactcccggc tgaagaagcg gaagtatttc ctggatgtgc
     5461 tggaatccga cctgatgcag ttcaagcaca tcagcagcaa cgagtacatc atcgaggaca
     5521 gcttcaagct gctgaacagc gagcagaaga atacactgct gaagtcttac aagtatatca
     5581 aagagagcgt cgagaacgat atcaagttcg cccaggaagg catcagctac tacgagaaag
     5641 tgctggccaa gtacaaggac gacctggaaa gcatcaagaa agtgatcaaa gaagaaaaag
     5701 agaagttccc cagcagcccc cctacaaccc ctcctagccc cgccaagacc gacgagcaga
     5761 aaaaagagag caagttcctg ccctttctga ccaacatcga gacactgtac aataacctcg
     5821 tgaacaagat cgatgattac ctgatcaatc tgaaggccaa gatcaatgac tgcaatgtgg
     5881 agaaggacga ggcccacgtg aagatcacca agctgtccga tctgaaagcc atcgacgaca
     5941 agatcgacct gtttaagaac ccctacgact ttgaggccat taaaaagctg atcaacgacg
     6001 ataccaagaa agacatgctg ggcaagctgc tgtccaccgg cctggtgcag aactttccca
     6061 acaccatcat cagcaagctg atcgaaggga agttccagga catgctgaat atcgcccagc
     6121 accagtgcgt gaagaaacag tgccccgaga acagcggctg cttccggcac ctggacgagc
     6181 gggaggaatg caagtgcctg ctgaactaca agcaagaggg cgataagtgc gtcgagaacc
     6241 ccaaccccac ctgtaacgag aacaacggcg gctgtgacgc cgatgccacc tgtaccgaag
     6301 aggacagcgg cagctcccgg aagaaaatca catgcgagtg caccaagccc gacagctacc
     6361 ccctgttcga cggcatcttc tgcagcagct ctggcgcgcc gtcgacctcc atcacggcct
     6421 ataagagtga gggggagtca gcggagttct ccttcccact caaccttgga gaggaaagcc
     6481 tgcagggaga gttgagatgg aaggcagaga aggctccttc ttcccagtcc tggatcacct
     6541 tctccctaaa gaaccaaaag gtgtctgtgc agaagtctac tagcaacccc aagttccagc
     6601 tgtccgaaac gctcccactc acccttcaga taccccaggt ctcccttcag tttgctggtt
     6661 ctggcaacct gaccctgact ctggacagag ggatactgta tcaggaagtg aacctggtgg
     6721 tgatgaaagt gactcagccc gacagcaaca ctttgacctg tgaggtgatg ggacccacct
     6781 cacccaagat gagactgatc ttgaagcagg agaatcagga ggccagggtc tccaggcagg
     6841 agaaagtgat tcaagtgcag gcccctgaag caggggtgtg gcaatgtcta ctgagtgaag
     6901 gtgaagaggt caagatggac tccaagatcc aggttttatc caaagggttg aattccggat
     6961 cactgcatca tattctggat gcacagaaaa tggtgtggaa tcatcgttaa gtctagacac
     7021 gtgattaatt aaggatcccc cgacctcgac ctctggctaa taaaggaaat ttattttcat
     7081 tgcaatagtg tgttggaatt ttttgtgtct ctcactcgga aggacatatg ggagggcaaa
     7141 tcatttggtc gagatccctc ggagatctct agctagagga tcgatccccg ccccggacga
     7201 actaaacctg actacgacat ctctgcccct tcttcgcggg gcagtgcatg taatcccttc
     7261 agttggttgg tacaacttgc caactgggcc ctgttccaca tgtgacacgg ggggggacca
     7321 aacacaaagg ggttctctga ctgtagttga catccttata aatggatgtg cacatttgcc
     7381 aacactgagt ggctttcatc ctggagcaga ctttgcagtc tgtggactgc aacacaacat
     7441 tgcctttatg tgtaactctt ggctgaagct cttacaccaa tgctggggga catgtacctc
     7501 ccaggggccc aggaagacta cgggaggcta caccaacgtc aatcagaggg gcctgtgtag
     7561 ctaccgataa gcggaccctc aagagggcat tagcaatagt gtttataagg cccccttgtt
     7621 aaccctaaac gggtagcata tgcttcccgg gtagtagtat atactatcca gactaaccct
     7681 aattcaatag catatgttac ccaacgggaa gcatatgcta tcgaattagg gttagtaaaa
     7741 gggtcctaag gaacagcgat atctcccacc ccatgagctg tcacggtttt atttacatgg
     7801 ggtcaggatt ccacgagggt agtgaaccat tttagtcaca agggcagtgg ctgaagatca
     7861 aggagcgggc agtgaactct cctgaatctt cgcctgcttc ttcattctcc ttcgtttagc
     7921 taatagaata actgctgagt tgtgaacagt aaggtgtatg tgaggtgctc gaaaacaagg
     7981 tttcaggtga cgcccccaga ataaaatttg gacggggggt tcagtggtgg cattgtgcta
     8041 tgacaccaat ataaccctca caaacccctt gggcaataaa tactagtgta ggaatgaaac
     8101 attctgaata tctttaacaa tagaaatcca tggggtgggg acaagccgta aagactggat
     8161 gtccatctca cacgaattta tggctatggg caacacataa tcctagtgca atatgatact
     8221 ggggttatta agatgtgtcc caggcaggga ccaagacagg tgaaccatgt tgttacactc
     8281 tatttgtaac aaggggaaag agagtggacg ccgacagcag cggactccac tggttgtctc
     8341 taacaccccc gaaaattaaa cggggctcca cgccaatggg gcccataaac aaagacaagt
     8401 ggccactctt ttttttgaaa ttgtggagtg ggggcacgcg tcagccccca cacgccgccc
     8461 tgcggttttg gactgtaaaa taagggtgta ataacttggc tgattgtaac cccgctaacc
     8521 actgcggtca aaccacttgc ccacaaaacc actaatggca ccccggggaa tacctgcata
     8581 agtaggtggg cgggccaaga taggggcgcg attgctgcga tctggaggac aaattacaca
     8641 cacttgcgcc tgagcgccaa gcacagggtt gttggtcctc atattcacga ggtcgctgag
     8701 agcacggtgg gctaatgttg ccatgggtag catatactac ccaaatatct ggatagcata
     8761 tgctatccta atctatatct gggtagcata ggctatccta atctatatct gggtagcata
     8821 tgctatccta atctatatct gggtagtata tgctatccta atttatatct gggtagcata
     8881 ggctatccta atctatatct gggtagcata tgctatccta atctatatct gggtagtata
     8941 tgctatccta atctgtatcc gggtagcata tgctatccta atagagatta gggtagtata
     9001 tgctatccta atttatatct gggtagcata tactacccaa atatctggat agcatatgct
     9061 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agcataggct
     9121 atcctaatct atatctgggt agcatatgct atcctaatct atatctgggt agtatatgct
     9181 atcctaattt atatctgggt agcataggct atcctaatct atatctgggt agcatatgct
     9241 atcctaatct atatctgggt agtatatgct atcctaatct gtatccgggt agcatatgct
     9301 atcctcatga taagctgtca aacatgagaa ttttcttgaa gacgaaaggg cctcgtgata
     9361 cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
     9421 tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
     9481 tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
     9541 atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
     9601 gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
     9661 cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
     9721 gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
     9781 cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
     9841 gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
     9901 tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
     9961 ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
    10021 gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
    10081 cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
    10141 tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
    10201 tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
    10261 cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
    10321 acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
    10381 tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
    10441 ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
    10501 accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
    10561 aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
    10621 ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
    10681 gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta gccgtagtta
    10741 ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
    10801 ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
    10861 ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
    10921 gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
    10981 cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
    11041 cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
    11101 cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
    11161 aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
    11221 ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
    11281 gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
    11341 gagcgcccaa tacgcaaacc gcctctcccc gcgcgttggc cgattcatta atgcagctgg
    11401 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
    11461 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
    11521 attgtgagcg gataacaatt tcacacagga aacagctatg accatgatta cgccaagctc
    11581 tagctagagg tcgaccaatt ctcatgtttg acagcttatc atcgcagatc cgggcaacgt
    11641 tgttgccatt gctgcaggcg cagaactggt aggtatgga
//
