LOCUS       sequence-6832-ex        8111 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8111)
  TITLE     Unpublished, Paco
REFERENCE   2  (bases 1 to 8111)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: YEplac181-BUD32
FEATURES             Location/Qualifiers
     source          1..8111
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    106..127
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(142..147,148..165,166..172)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    180..196
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     185..207
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     204..220
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     204..220
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(2660..2677)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(2660..2676)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2669..2691)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      3113..4459
                     /label=2u ori
                     /note="yeast 2u plasmid origin of replication"
     promoter        complement(5701..6099)
                     /gene="S. cerevisiae LEU2"
                     /label=LEU2 promoter
     primer_bind     complement(6119..6137)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        6205..6309
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(6310..6378,6379..7170)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6528..6547)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      7341..7929
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7830..7849
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     8083..8100
                     /label=L4440
                     /note="L4440 vector, forward primer"
ORIGIN
        1 gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat gcagctggca
       61 cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg tgagttagct
      121 cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt tgtgtggaat
      181 tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg ccaagcttgc
      241 atgcctgcag gtcgactcta gagttcctag aatttaacaa ggtgactaac tgttgaggat
      301 tgatgttttg agaataataa gtataggagg attcacccag cttagaggtg gctaccgcgg
      361 cagcttccct cgttattact ttggccgtat ccaacgccga tgcaatacgg tgaattgaat
      421 ctaccattgc tggttgtcca cttttcgatt gtttgctgtc tttggtttac ctttcttttc
      481 tttatatatt tatatatatg gttcttcaaa aatgatttgt ctgaatttgt caaaatctca
      541 ataactcgga cacgctttga tggtaacata cctaaaagtt atgtacgtta atataaatta
      601 tagatacata ttatgtgcag cgatatacag gcagtacgct agcatttata ttatcctagc
      661 atacttctct tacgaccacg caacctgacc tcttcgaatc ttttggtaac ttctttcagt
      721 ttcttggcac ctttcgcacc ttgttcacga tagacctctt cgaacccctc catgatccaa
      781 gcattgtatt tttccgcatg cttcgaatgt gtacttaaaa tagctctctc taagacgtat
      841 aaatcgacgc ctttatcttc gaccaggttt gaaactgagc ccagaccgaa atcaatcaaa
      901 tgaggcgtcc atctcgcacc atctcgcacg agaacaatgt tagaacttgt caaatcacca
      961 tgacagtagt cattccagtg caacaaccca atttggcgcc ccactttccg tagtgtagtt
     1021 gctacaagat cactatatgg atcttggtca tgcatccaaa ggaagttctt taggttacta
     1081 aaaccgtgcc ctccgggaag atcttctcca aggaactcta accaaatgaa tccattgtat
     1141 ggatcacacg ctatcagttg agggacacat agccctggaa tcaagtataa tttggccaat
     1201 aaacgcgact cattcaacgt acgatgtttt gttagtgcct ggtctatttg tggatgtcta
     1261 taacgctttg gtggcctata tttgataata tacttttgat gagaatcctt tgctcttggg
     1321 agatatgggt gagtagtcgt tgtgaaaaca atggcttctg caccttgaga aataggtgca
     1381 atgtccacat caggagtcag gtaggaagat actttatcaa tgaattcttg cgtcataatg
     1441 agcaaagcgt ataacctttt ccgctgtcgt attcatcact tatttgattt caagtaaggt
     1501 tatctttgtc cttcaagttg gtgatgcgcc ggcgattttt tcaaccttgt tgctgctaaa
     1561 ataatggaaa atcaaaataa atttgtgctg acccaaatat ttatgtatat aaaaggggtt
     1621 atgtaattgt cattatgaca aattcaggat tgcataaact caagaatgcg agcgatagat
     1681 ggtatgttgg tccagcgatc catattacga gagtagttta atattgggcc cgtatgataa
     1741 ccttggtgtt caaccagcac atttttggcg gtaaagtggt tacatccctc gctagcgtca
     1801 ctcattttct ttgccaagat cttgttttga aagctcaaca actcatcact accataagta
     1861 atcatcacag agcacttttt cagaatatct tgccacattt tagtttcgaa attcttttca
     1921 atattaacaa aagggtcgct ggtaaaatcg acgtttgggt tgtttcctac aaacattcgc
     1981 ccaaacatcg aatgactctt caaacataga ccatcccatt cttgtgttcc ttgcatatat
     2041 tccttttctc cctcgtggaa gaaagtggca ttggcccagg gacttatggc tatgactttt
     2101 ttcgggtata tttctctgtg acactttctt aaatataaaa caatgttcaa aacagcattg
     2161 ccaccagcag aatcccccat aatcactaca tttttgcacc ccatagtctt aacaacgtaa
     2221 tcaaaaatgg ccacattttg taaaatttgc agagggtact ttttcgactg gtcatcggta
     2281 gctgtaacgg tataatctgg tacaagaatg gccattttag gaaaggcatt tcgcatatta
     2341 ttcaagaaag aaaacgacgt aggcgtcaat ttaagagcat aaccaccccc atgatagaat
     2401 actaaaactg gatctgtcgt ggggtcaaat ccttccggca taacatattg ataaaacttt
     2461 gtgctaacta ttttgggtgc gtttaattga tggtcttgga aaatattgtt ttctggagat
     2521 ttctgatctt ctataggaat gttgcatttg acatatgttg gactagaaaa tgtagaagga
     2581 tataaacaaa caaataaagg attgagcacg tattgacaga tgcgttcatc gaggatcccc
     2641 gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     2701 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     2761 aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
     2821 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tatcggatcg
     2881 tacttgttac ccatcattga attttgaaca tccgaacctg ggagttttcc ctgaaacaga
     2941 tagtatattt gaacctgtat aataatatat agtctagcgc tttacggaag acaatgtatg
     3001 tatttcggtt cctggagaaa ctattgcatc tattgcatag gtaatcttgc acgtcgcatc
     3061 cccggttcat tttctgcgtt tccatcttgc acttcaatag catatctttg ttaacgaagc
     3121 atctgtgctt cattttgtag aacaaaaatg caacgcgaga gcgctaattt ttcaaacaaa
     3181 gaatctgagc tgcattttta cagaacagaa atgcaacgcg aaagcgctat tttaccaacg
     3241 aagaatctgt gcttcatttt tgtaaaacaa aaatgcaacg cgagagcgct aatttttcaa
     3301 acaaagaatc tgagctgcat ttttacagaa cagaaatgca acgcgagagc gctattttac
     3361 caacaaagaa tctatacttc ttttttgttc tacaaaaatg catcccgaga gcgctatttt
     3421 tctaacaaag catcttagat tacttttttt ctcctttgtg cgctctataa tgcagtctct
     3481 tgataacttt ttgcactgta ggtccgttaa ggttagaaga aggctacttt ggtgtctatt
     3541 ttctcttcca taaaaaaagc ctgactccac ttcccgcgtt tactgattac tagcgaagct
     3601 gcgggtgcat tttttcaaga taaaggcatc cccgattata ttctataccg atgtggattg
     3661 cgcatacttt gtgaacagaa agtgatagcg ttgatgattc ttcattggtc agaaaattat
     3721 gaacggtttc ttctattttg tctctatata ctacgtatag gaaatgttta cattttcgta
     3781 ttgttttcga ttcactctat gaatagttct tactacaatt tttttgtcta aagagtaata
     3841 ctagagataa acataaaaaa tgtagaggtc gagtttagat gcaagttcaa ggagcgaaag
     3901 gtggatgggt aggttatata gggatatagc acagagatat atagcaaaga gatacttttg
     3961 agcaatgttt gtggaagcgg tattcgcaat attttagtag ctcgttacag tccggtgcgt
     4021 ttttggtttt ttgaaagtgc gtcttcagag cgcttttggt tttcaaaagc gctctgaagt
     4081 tcctatactt tctagctaga gaataggaac ttcggaatag gaacttcaaa gcgtttccga
     4141 aaacgagcgc ttccgaaaat gcaacgcgag ctgcgcacat acagctcact gttcacgtcg
     4201 cacctatatc tgcgtgttgc ctgtatatat atatacatga gaagaacggc atagtgcgtg
     4261 tttatgctta aatgcgtact tatatgcgtc tatttatgta ggatgaaagg tagtctagta
     4321 cctcctgtga tattatccca ttccatgcgg ggtatcgtat gcttccttca gcactaccct
     4381 ttagctgttc tatatgctgc cactcctcaa ttggattagt ctcatccttc aatgctatca
     4441 tttcctttga tattggatcg atccgatgat aagctgtcaa acatgagaat taattctacc
     4501 ctatgaacat attccatttt gtaatttcgt gtcgtttcta ttatgaattt catttataaa
     4561 gtttatgtac aaatatcata aaaaaagaga atctttttaa gcaaggattt tcttaacttc
     4621 ttcggcgaca gcatcaccga cttcggtggt actgttggaa ccacctaaat caccagttct
     4681 gatacctgca tccaaaacct ttttaactgc atcttcaatg gccttacctt cttcaggcaa
     4741 gttcaatgac aatttcaaca tcattgcagc agacaagata gtggcgatag ggtcaacctt
     4801 attctttggc aaatctggag cagaaccgtg gcatggttcg tacaaaccaa atgcggtgtt
     4861 cttgtctggc aaagaggcca aggacgcaga tggcaacaaa cccaaggaac ctgggataac
     4921 ggaggcttca tcggagatga tatcaccaaa catgttgctg gtgattataa taccatttag
     4981 gtgggttggg ttcttaacta ggatcatggc ggcagaatca atcaattgat gttgaacctt
     5041 caatgtagga aattcgttct tgatggtttc ctccacagtt tttctccata atcttgaaga
     5101 ggccaaaaca ttagctttat ccaaggacca aataggcaat ggtggctcat gttgtagggc
     5161 catgaaagcg gccattcttg tgattctttg cacttctgga acggtgtatt gttcactatc
     5221 ccaagcgaca ccatcaccat cgtcttcctt tctcttacca aagtaaatac ctcccactaa
     5281 ttctctgaca acaacgaagt cagtaccttt agcaaattgt ggcttgattg gagataagtc
     5341 taaaagagag tcggatgcaa agttacatgg tcttaagttg gcgtacaatt gaagttcttt
     5401 acggattttt agtaaacctt gttcaggtct aacactacct gtaccccatt taggaccacc
     5461 cacagcacct aacaaaacgg catcaacctt cttggaggct tccagcgcct catctggaag
     5521 tgggacacct gtagcatcga tagcagcacc accaattaaa tgattttcga aatcgaactt
     5581 gacattggaa cgaacatcag aaatagcttt aagaacctta atggcttcgg ctgtgatctt
     5641 gaccaacgtg gtcacctggc aaaacgacga tcttcttagg ggcagacata ggggcagaca
     5701 ttagaatgta tatccttgaa atatatatat atattgctga aatgtaaaag gtaagaaaag
     5761 ttagaataag acgattgcta accacctatt ggaaaaaaca ataggtcctt aaataatatt
     5821 gtcaacttca agtattgtga tgcaagcatt tagtcatgaa cgcttctcta ttctatatga
     5881 aaagccggtt ccggcctctc acctttcctt tttctcccaa tttttcagtt gaaaaaggta
     5941 tatgcgcggc gacctctgaa attaacaaaa aatttccagt catcgaattt gtctgtgcga
     6001 tagcgcccct gtgtgttctc gttatgttga ggaaaaaaat aatggttgct aagagattcg
     6061 aactcttgca tcttacgata cctgagtatt cccacagtta attcttgaag acgaaagggc
     6121 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     6181 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     6241 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     6301 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     6361 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     6421 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     6481 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     6541 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     6601 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     6661 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     6721 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     6781 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     6841 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     6901 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     6961 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     7021 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     7081 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     7141 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     7201 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     7261 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     7321 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     7381 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     7441 tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag
     7501 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     7561 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     7621 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     7681 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     7741 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     7801 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     7861 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     7921 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     7981 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     8041 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     8101 gaagcggaag a
//
