LOCUS       sequence-68328-e         915 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 915)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 915)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin I SH3 B domain (human)
FEATURES             Location/Qualifiers
     source          1..915
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(311..333)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(478..496)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        564..668
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(887..906)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccccgg aattcggtga aaaggtggag gggctacaag
       61 ctcaagccct atatccttgg agagccaaaa aagacaacca cttaaatttt aacaaaaatg
      121 atgtcatcac cgtcctggaa cagcaagaca tgtggtggtt tggagaagtt caaggtcaga
      181 agggttggtt ccccaagtct tacgtgaaac tcatttcagg gcccataagg aagtctacaa
      241 gctgactcga gcggccgcat cgtgactgac tgacgatctg cctcgcgcgt ttcggtgatg
      301 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
      361 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcggg tgtcggggcg
      421 cagccatgac ccagtcacgt agcgatagcg gagtgtataa ttcttgaaga cgaaagggcc
      481 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
      541 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
      601 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
      661 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
      721 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
      781 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
      841 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
      901 tattatcccg tgttg
//
