LOCUS       sequence-68338-e         786 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  encodes Flag-tagged mouse IL-17RA fused to Yellow Fl. Protein.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 786)
  AUTHORS   Maitra A, Shen F, Hanel W, Mossman K, Tocker J, Swart D, Gaffen SL
  TITLE     Distinct functional motifs within the IL-17 receptor regulate signal
            transduction and target gene expression.
  JOURNAL   Proc Natl Acad Sci U S A. 2007 May 1;104(18):7506-11. Epub 2007 Apr 
            24.
  PUBMED    17456598
REFERENCE   2  (bases 1 to 786)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Proc Natl 
            Acad Sci U S A."; date: "2007-05-1"; volume: "104(18)"; pages: 
            "7506-11. Epub 2007 Apr 24"
COMMENT     Sequence Label: pCMV4-Flag-mIL-17RA/YFP.Hygro
FEATURES             Location/Qualifiers
     source          1..786
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(187..438)
                     /codon_start=1
                     /product="C-terminal fragment of mVenus for use in 
                     bimolecular fluorescence complementation (BiFC) (Kodama and
                     Hu, 2010)"
                     /label=VC155
                     /translation="DKQKNGIKVNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNH
                     YLSYQSALSKDPNEKRDHMVLLEFVTAAGITLGMDELYK"
     primer_bind     complement(229..250)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     578..597
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 ccttgctcca taccaccccc ctccacccca taatattata gaaggacacc tagatcagac
       61 aaaatgatgc aacttaattt tattaggaca aggctggtgg gcactggagt ggcaacttcc
      121 agggccagga gaggcactgg ggaggggtca cagggatgcc acccgggatc ctctagagtc
      181 gacttacttg tacagctcgt ccatgccgag agtgatcccg gcggcggtca cgaactccag
      241 caggaccatg tgatcgcgct tctcgttggg gtctttgctc agggcggact ggtagctcag
      301 gtagtggttg tcgggcagca gcacggggcc gtcgccgatg ggggtgttct gctggtagtg
      361 gtcggcgagc tgcacgctgc cgtcctcgat gttgtggcgg atcttgaagt tcaccttgat
      421 gccgttcttc tgcttgtcgg ccatgatata gacgttgtgg ctgttgtagt tgtactccag
      481 cttgtgcccc aggatgttgc cgtcctcctt gaagtcgatg cccttcagct cgatgcggtt
      541 caccagggtg tcgccctcga acttcacctc ggcgcgggtc ttgtagttgc cgtcgtcctt
      601 gaagaagatg gtgcgctcct ggacgtagcc ttcgggcatg gcggacttga agaagtcgtg
      661 ctgcttcatg tggtcggggt agcgggcgaa gcacttcagg ccgtagccga aggtggtcac
      721 gagggtgggc cagggcacgg gcagcttgcc ggtggtgcag atgaacttca gggtcagctt
      781 gccgta
//
