LOCUS       sequence-68395-e         905 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 905)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 905)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin I SH3 C domain (human)
FEATURES             Location/Qualifiers
     source          1..905
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(305..327)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(472..490)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        558..662
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(881..900)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccccgg aattcccggt cgtttcggga gaagaattta
       61 ttgccatgta cacttacgag agttctgagc aaggagattt aacctttcag caaggggatg
      121 tgattttggt taccaagaaa gatggtgact ggtggacagg aacagtgggc gacaaggccg
      181 gagtcttccc ttctaactat gtgaggctta aagattcaga gggctctgga actgcttgac
      241 tcgagcggcc gcatcgtgac tgactgacga tctgcctcgc gcgtttcggt gatgacggtg
      301 aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg
      361 ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca
      421 tgacccagtc acgtagcgat agcggagtgt ataattcttg aagacgaaag ggcctcgtga
      481 tacgcctatt tttataggtt aatgtcatga taataatggt ttcttagacg tcaggtggca
      541 cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt tttctaaata cattcaaata
      601 tgtatccgct catgagacaa taaccctgat aaatgcttca ataatattga aaaaggaaga
      661 gtatgagtat tcaacatttc cgtgtcgccc ttattccctt ttttgcggca ttttgccttc
      721 ctgtttttgc tcacccagaa acgctggtga aagtaaaaga tgctgaagat cagttgggtg
      781 cacgagtggg ttacatcgaa ctggatctca acagcggtaa gatccttgag agttttcgcc
      841 ccgaagaacg ttttccaatg atgagcactt ttaaagttct gctatgtggc gcggtattat
      901 cccgt
//
