LOCUS       sequence-68398-e         936 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 936)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 936)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin I SH3 D domain (human)
FEATURES             Location/Qualifiers
     source          1..936
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(338..360)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(505..523)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        591..695
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(914..933)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccccgg aattcgggag tttaggaaaa aaacctgaaa
       61 ttgcccaggt tattgcctca tacaccgcca ccggccccga gcagctcact ctcgcccctg
      121 gtcagctgat tttgatccga aaaaagaacc caggtggatg gtgggaagga gagctgcaag
      181 cacgtgggaa aaagcgccag ataggctggt tcccagctaa ttatgtaaag cttctaagcc
      241 ctgggacgag caaaatcact ccaacagagt gactcgagcg gccgcatcgt gactgactga
      301 cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
      361 agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
      421 cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
      481 tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
      541 tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
      601 ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
      661 gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
      721 cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
      781 tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
      841 tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
      901 cttttaaagt tctgctatgt ggcgcggtat tatccc
//
