LOCUS       sequence-68399-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Yamabhai M, Hoffman NG, Hardison NL, McPherson PS, Castagnoli L, 
            Cesareni G, Kay BK
  TITLE     Intersectin, a novel adaptor protein with two Eps15 homology and 
            five Src homology 3 domains.
  JOURNAL   J Biol Chem. 1998 Nov 20;273(47):31401-7.
  PUBMED    9813051
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem."; date: "1998-11-20"; volume: "273(47)"; pages: "31401-7"
COMMENT     Sequence Label: Intersectin I SH3 E domain (human)
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             9..26
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     primer_bind     complement(302..324)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(469..487)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        555..659
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(878..897)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
ORIGIN
        1 aatcggatct ggttccgcgt ggatccccgg aattcgcggc agtgtgccag gtgattggga
       61 tgtacgacta caccgcgcag aatgacgatg agctggcctt caacaagggc cagatcatca
      121 acgtcctcaa caaggaggac cctgactggt ggaaaggaga agtcaatgga caagtggggc
      181 tcttcccatc caattatgtg aagctgacca cagacatgga cccaagccag caatgactcg
      241 agcggccgca tcgtgactga ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa
      301 acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
      361 gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga
      421 cccagtcacg tagcgatagc ggagtgtata attcttgaag acgaaagggc ctcgtgatac
      481 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
      541 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
      601 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
      661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
      721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
      781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
      841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
      901 gtgttgacgc cgggcaagaa caactcggtc gccgcatac
//
