LOCUS       sequence-68405-e         972 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 972)
  AUTHORS   Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, 
            Fotouhi M, Maret D, Ritter B, Del Maestro RF, McPherson PS
  TITLE     Interplay between Rab35 and Arf6 controls cargo recycling to 
            coordinate cell adhesion and migration.
  JOURNAL   J Cell Sci. 2013 Feb 1;126(Pt 3):722-31. doi: 10.1242/jcs.112375. 
            Epub 2012 Dec 21.
  PUBMED    23264734
REFERENCE   2  (bases 1 to 972)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-02-1"; volume: "126(Pt 3):722-31. doi"; pages: " 
            10.1242/jcs.112375. Epub 2012 Dec 21"
COMMENT     Sequence Label: Flag-Rab35 S22N inactive
FEATURES             Location/Qualifiers
     source          1..972
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             91..114
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     promoter        complement(787..805)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(788..805)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(825..842)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    831..942
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gactacaaag acgatgacga caagggatcc
      121 atggcccggg actacgacca cctcttcaag ctgctcatca tcggcgacag cggtgtgggc
      181 aagaacagtt tactgttgcg ttttgcagac aacactttct caggcagcta catcaccacg
      241 atcggagtgg atttcaagat ccggaccgtg gagatcaacg gggagaaggt gaagctgcag
      301 atctgggaca cagcggggca ggagcgcttc cgcaccatca cctccacgta ttatcggggg
      361 acccacgggg tcattgtggt ttacgacgtc accagtgccg agtcctttgt caacgtcaag
      421 cggtggcttc acgaaatcaa ccagaactgt gatgatgtgt gccgaatatt agtgggtaat
      481 aagaatgacg accctgagcg gaaggtggtg gagacggaag atgcctacaa attcgccggg
      541 cagatgggca tccagttgtt cgagaccagc gccaaggaga atgtcaacgt ggaagagatg
      601 ttcaactgca tcacggagct ggtcctccga gcaaagaaag acaacctggc aaaacagcag
      661 cagcaacaac agaacgatgt ggtgaagctc acgaagaaca gtaaacgaaa gaaacgctgc
      721 tgctaagaat tctgcagata tccatcacac tggcggccgc tcgagcatgc atctagaggg
      781 ccctattcta tagtgtcacc taaatgctag agctcgctga tcagcctcga ctgtgccttc
      841 tagttgccag ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc
      901 cactcccact gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg
      961 tcattctatt ct
//
