LOCUS       sequence-68407-e         880 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 880)
  TITLE     McPherson Lab unplublished
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 880)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: Flag-WASP
FEATURES             Location/Qualifiers
     source          1..880
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             50..73
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
ORIGIN
        1 gtccactccc aggtccaact gcacctcggt tctatcgcta gccaccatgg actacaagga
       61 cgatgacgat aagggatccc cgggaatttc cggtggtggt ggtggaattc tagactccat
      121 gggtgggggc ccaatgggag gaaggcccgg gggccgagga gcaccagcgg ttcagcagaa
      181 cataccctcc accctcctcc aggaccacga gaaccagcga ctctttgaga tgcttggacg
      241 aaaatgcttg acgctggcca ctgcagttgt tcagctgtac ctggcgctgc cccctggagc
      301 tgagcactgg accaaggagc attgtggggc tgtgtgcttc gtgaaggata acccccagaa
      361 gtcctacttc atccgccttt acggccttca ggctggtcgg ctgctctggg aacaggagct
      421 gtactcacag cttgtctact ccacccccac ccccttcttc cacaccttcg ctggagatga
      481 ctgccaagcg gggctgaact ttgcagacga ggacgaggcc caggccttcc gggccctcgt
      541 gcaggagaag atacaaaaaa ggaatcagag gcaaagtgga gacagacgcc agctaccccc
      601 accaccaaca ccagccaatg aagagagaag aggagggctc ccacccctgc ccctgcatcc
      661 aggtggagac caaggaggcc ctccagtggg tccgctctcc ctggggctgg cgacagtgga
      721 catccagaac cctgacatca cgagttcacg ataccgtggg ctcccagcac ctggacctag
      781 cccagctgat aagaaacgct cagggaagaa gaagatcagc aaagctgata ttggtgcacc
      841 cagtggattc aagcatgtca gccacgtggg gtgggacccc
//
