LOCUS       sequence-68410-e         939 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 939)
  AUTHORS   Allaire PD, Seyed Sadr M, Chaineau M, Seyed Sadr E, Konefal S, 
            Fotouhi M, Maret D, Ritter B, Del Maestro RF, McPherson PS
  TITLE     Interplay between Rab35 and Arf6 controls cargo recycling to 
            coordinate cell adhesion and migration.
  JOURNAL   J Cell Sci. 2013 Feb 1;126(Pt 3):722-31. doi: 10.1242/jcs.112375. 
            Epub 2012 Dec 21.
  PUBMED    23264734
REFERENCE   2  (bases 1 to 939)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Sci."; date: "2013-02-1"; volume: "126(Pt 3):722-31. doi"; pages: " 
            10.1242/jcs.112375. Epub 2012 Dec 21"
COMMENT     Sequence Label: Myc-Rab35 S22N
FEATURES             Location/Qualifiers
     source          1..939
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     50..69
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        50..68
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     regulatory      82..91
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     promoter        complement(799..817)
                     /label=SP6 promoter
                     /note="promoter for bacteriophage SP6 RNA polymerase"
     primer_bind     complement(800..817)
                     /label=SP6
                     /note="SP6 promoter, forward primer"
     primer_bind     complement(837..854)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
ORIGIN
        1 gagctctctg gctaactaga gaacccactg cttactggct tatcgaaatt aatacgactc
       61 actataggga gacccaagct tgccaccatg gcatcaatgc agaagctgat ctcagaggag
      121 gacctgggat ccatggcccg ggactacgac cacctcttca agctgctcat catcggcgac
      181 agcggtgtgg gcaagaacag tttactgttg cgttttgcag acaacacttt ctcaggcagc
      241 tacatcacca cgatcggagt ggatttcaag atccggaccg tggagatcaa cggggagaag
      301 gtgaagctgc agatctggga cacagcgggg caggagcgct tccgcaccat cacctccacg
      361 tattatcggg ggacccacgg ggtcattgtg gtttacgacg tcaccagtgc cgagtccttt
      421 gtcaacgtca agcggtggct tcacgaaatc aaccagaact gtgatgatgt gtgccgaata
      481 ttagtgggta ataagaatga cgaccctgag cggaaggtgg tggagacgga agatgcctac
      541 aaattcgccg ggcagatggg catccagttg ttcgagacca gcgccaagga gaatgtcaac
      601 gtggaagaga tgttcaactg catcacggag ctggtcctcc gagcaaagaa agacaacctg
      661 gcaaaacagc agcagcaaca acagaacgat gtggtgaagc tcacgaagaa cagtaaacga
      721 aagaaacgct gctgctaaga attctgcaga tatccatcac actggcggcc gctcgagcat
      781 gcatctagag ggccctattc tatagtgtca cctaaatgct agagctcgct gatcagcctc
      841 gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc cttccttgac
      901 cctggaaggt gccactccca ctgtcctttc ctaataaaa
//
