LOCUS       sequence-68423-e         874 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Vector modified from pBluescript SK(+) by adding more cloning sites.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 874)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 874)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX1
FEATURES             Location/Qualifiers
     source          1..874
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     15..35
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        17..35
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     72..88
                     /label=SK primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     72..88
                     /label=pBluescriptSK
                     /note="For pBluescript vector"
     primer_bind     complement(122..138)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(123..139)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     primer_bind     complement(182..201)
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        complement(183..201)
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     primer_bind     complement(211..228)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(211..227)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(220..242)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     rep_origin      369..824
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(456..475)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     666..687
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 attacgccaa gcgcgcaatt aaccctcact aaagggaaca aaagctggag ctccaccgcg
       61 gtggcggccg ctctagaact agtggatccc ccgggctgca ggaattcgat atcaagctta
      121 tcgataccgt cgacctcgag gcgcgccaga tcttaattaa ggccggccgg tacccaattc
      181 gccctatagt gagtcgtatt acgcgcgctc actggccgtc gttttacaac gtcgtgactg
      241 ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg
      301 gcgtaatagc gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg
      361 cgaatgggac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
      421 cgtgaccgct acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt
      481 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
      541 ccgatttagt gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg
      601 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
      661 taatagtgga ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt
      721 tgatttataa gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca
      781 aaaatttaac gcgaatttta acaaaatatt aacgcttaca atttaggtgg cacttttcgg
      841 ggaaatgtgc gcggaacccc tatttgttta tttt
//
