LOCUS       sequence-68429-e         656 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP RFP op. PGK-puro indicator plasmid to assay TOP IMCE efficiency 
            .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 656)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 656)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX28
FEATURES             Location/Qualifiers
     source          1..656
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      441..450
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     primer_bind     complement(580..598)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
ORIGIN
        1 gccagggcgt gcccttgggc tccccgggcg cgtactccac ctcacccatc tgaagcttcc
       61 gacttcttgg atacttgtaa ctttgcgctt tcccaccctc gcataagttt cctgaaataa
      121 tgactctgaa actcaaaata tatttacaaa cacctcggct gtagctttgg tttgttctct
      181 gacttgctta taacttaata tccctcttat tctaacctaa gttctgccac gtggttggtt
      241 acctctgctc agcccccggc ttctgtcctc catgttcctg ggggaatccc tacactttca
      301 gaatttaatt tccctactgg atgtcccacc ttctttttat tctacccttt cctataagcc
      361 ataggggttt gtttgtttgt atgtttttta attgacaagt tatgcatcca tacagtacac
      421 aatctcttct ctctacagca gccaccatgg tgagcaaggg cgaggaggtc atcaaagagt
      481 tcatgcgctt caaggtgcgc atggagggct ccatgaacgg ccacgagttc gagatcgagg
      541 gcgagggcga gggccgcccc tacgagggca cccagaccgc caagctgaag gtgaccaagg
      601 gcggccccct gcccttcgcc tgggacatcc tgtcccccca gttcatgtac ggctcc
//
