LOCUS       sequence-68430-e         952 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-wt plasmid without selection marker.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 952)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 952)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX41
FEATURES             Location/Qualifiers
     source          1..952
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..43
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
ORIGIN
        1 cgcgggcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
       61 ctgaagcttc cgacttcttg gatacttgta actttgcgct ttcccaccct cgcataagtt
      121 tcctgaaata atgactctga aactcaaaat atatttacaa acacctcggc tgtagctttg
      181 gtttgttctc tgacttgctt ataacttaat atccctctta ttctaaccta agttctgcca
      241 cgtggttggt tacctctgct cagcccccgg cttctgtcct ccatgttcct gggggaatcc
      301 ctacactttc agaatttaat ttccctactg gatgtcccac cttcttttta ttctaccctt
      361 tcctataagc cataggggtt tgtttgtttg tatgtttttt aattgacaag ttatgcatcc
      421 atacagtaca caatctcttc tctctacaga tgactgccat ggaggagtca cagtcggata
      481 tcagcctcga gctccctctg agccaggaga cattttcagg cttatggaaa ctgtgagtgg
      541 atctttttgg ggcccttaag atacatcccg ccatacctgt atcctcccct tgcctgagag
      601 aaacaaaaac agtagtgttc aaacatggta tggtgttggg tgtctgtaaa tcctgcgggg
      661 cggggtggcg gggggggggg gactgcaggg tctcagaagt ttgaggtcat cattgactac
      721 atagcaagtt ggaggccagc ctgggataag tgagattctg tcttcaaaaa atggaaggaa
      781 atcaggaact aactctctgc tcttgttttc cagacttcct ccagaagata tcctggtaag
      841 gcccagagca gaaagggact tgggctttgg tgaattcctg cagcccgggg gatcctgagg
      901 acctggtcct ctgactgctc ttttcaccca tctacagtcc cccttgccgt cc
//
