LOCUS       sequence-68432-e         953 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-Hupki-wt plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 953)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 953)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX61
FEATURES             Location/Qualifiers
     source          1..953
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    186..255
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="attB site for the phi-C31 integrase (Groth et al., 
                     2000)"
ORIGIN
        1 cccgccgtga ccgtcgagaa cccgctgacg ctgccccgcg tatccgcacc cgccgacgcc
       61 gtcgcacgtc ccgtgctcac cgtgaccacc gcgcccagcg gtttcgaggg cgagggcttc
      121 ccggtgcgcc gcgcgttcgc cgggatcaac taccgccacc tcgacccgtt catcatgatg
      181 gaccagatgg gtgaggtgga gtacgcgccc ggggagccca agggcacgcc ctggcacccg
      241 caccgcggct tcgagaccgt gacctacatc gtctctagtg cggccgcacg gcattttgag
      301 tgttagactg gaaactttcc acttgataag aggtcccaag acttagtacc tgaagggtga
      361 aatattctcc atccagtggt ttcttctttg gctggggaga ggagctggtg ttgttgggca
      421 gtgctaggaa agaggcaagg aaaggtgata aaagtgaatc tgaggcataa ctgcaccctt
      481 ggcctcctcc accgcttctt gtcctgcttg cttacctcgc ttagtgctcc ctgggggcag
      541 ctcgtggtga ggctcccctt tcttgcggag attctcttcc tctgtgcgcc ggtctctccc
      601 aggacaggca caaacacgca cctcaaagct gttccgtccc agtagattac cactactcag
      661 gataggaaaa gagaagcaag aggcagtaag gaaatcaggt cctacctgtc ccatttaaaa
      721 aaccagactc catctactcc caaccaccct tgtcctttct ggagcctaag ctccagctcc
      781 aggtaggtgg aggagaagcc acaggttaag aggtcccaaa gccagagaaa agaaaactga
      841 gtgggagcag taaggagatt ccccgccggg gatgtgatga gaggtggatg ggtagtagta
      901 tggaagaaat cggtaagagg tgggcccagg ggtcagaggc aagcagaggc tgg
//
