LOCUS       sequence-68433-e         926 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-Hupki-wt plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 926)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 926)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX61
FEATURES             Location/Qualifiers
     source          1..926
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             106..159
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
ORIGIN
        1 gccgacgtcg aggtgcccga aggaccgcgc acctggtgca tgacccgcaa gcccggtgcc
       61 gggagcggcg gtggctctgg tggcggttca atggtgaggc tgctcgaggg cagaggaagt
      121 cttctaacat gcggtgacgt ggaggagaat cccggccctg caccgggagc caccatgact
      181 gccatggagg agtcacagtc ggatatcagc ctcgagctcc ctctgagcca ggagacattt
      241 tcaggcttat ggaaactgtg agtggatctt tttggggccc ttaagataca tcccgccata
      301 cctgtatcct ccccttgcct gagagaaaca aaaacagtag tgttcaaaca tggtatggtg
      361 ttgggtgtct gtaaatcctg cggggcgggg tggcgggggg gggggactgc agggtctcag
      421 aagtttgagg tcatcattga ctacatagca agttggaggc cagcctggga taagtgagat
      481 tctgtcttca aaaaatggaa ggaaatcagg aactaactct ctgctcttgt tttccagact
      541 tcctccagaa gatatcctgg taaggcccag agcagaaagg gacttgggct ttggtggatc
      601 ctgaggacct ggtcctctga ctgctctttt cacccatcta cagtccccct tgccgtccca
      661 agcaatggat gatttgatgc tgtccccgga cgatattgaa caatggttca ctgaagaccc
      721 aggtccagat gaagctccca gaatgccaga ggctgctccc cccgtggccc ctgcaccagc
      781 agctcctaca ccggcggccc ctgcaccagc cccctcctgg cccctgtcat cttctgtccc
      841 ttcccagaaa acctaccagg gcagctacgg tttccgtctg ggcttcttgc attctgggac
      901 agccaagtct gtgacttgca cggtca
//
