LOCUS       sequence-68434-e         992 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  TOP Hupki-puro2A-Hupki-wt plasmid .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 992)
  AUTHORS   Wei QX, Odell AF, van der Hoeven F, Hollstein M
  TITLE     Rapid derivation of genetically related mutants from embryonic cells
            harboring a recombinase-specific Trp53 platform.
  JOURNAL   Cell Cycle. 2011 Apr 15;10(8):1261-70. Epub 2011 Apr 15.
  PUBMED    21445009
REFERENCE   2  (bases 1 to 992)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell 
            Cycle."; date: "2011-04-15"; volume: "10(8)"; pages: "1261-70. Epub 
            2011 Apr 15"
COMMENT     Sequence Label: pX61
FEATURES             Location/Qualifiers
     source          1..992
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    10..43
                     /label=attB
                     /bound_moiety="phage phi-C31 integrase"
                     /note="minimal attB site for the phi-C31 integrase (Groth 
                     et al., 2000)"
     primer_bind     949..969
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
ORIGIN
        1 cgcgggcggg tgccagggcg tgcccttggg ctccccgggc gcgtactcca cctcacccat
       61 ctgaagcttc cgacttcttg gatacttgta actttgcgct ttcccaccct cgcataagtt
      121 tcctgaaata atgactctga aactcaaaat atatttacaa acacctcggc tgtagctttg
      181 gtttgttctc tgacttgctt ataacttaat atccctctta ttctaaccta agttctgcca
      241 cgtggttggt tacctctgct cagcccccgg cttctgtcct ccatgttcct gggggaatcc
      301 ctacactttc agaatttaat ttccctactg gatgtcccac cttcttttta ttctaccctt
      361 tcctataagc cataggggtt tgtttgtttg tatgtttttt aattgacaag ttatgcatcc
      421 atacagtaca caatctcttc tctctacaga tgggcaccga gtacaagccc acggtgcgcc
      481 tcgccacccg cgacgacgtc ccccgggccg tacgcaccct cgccgccgcg ttcgccgact
      541 accccgccac gcgccacacc gtcgacccgg accgccacat cgagcgggtc accgagctgc
      601 aagaactctt cctcacgcgc gtcgggctcg acatcggcaa ggtgtgggtc gcggacgacg
      661 gcgccgcggt ggcggtctgg accacgccgg agagcgtcga agcgggggcg gtgttcgccg
      721 agatcggccc gcgcatggcc gagttgagcg gttcccggct ggccgcgcag caacagatgg
      781 aaggcctcct ggcgccgcac cggcccaagg agcccgcgtg gttcctggcc accgtcggcg
      841 tctcgcccga ccaccagggc aagggtctgg gcagcgccgt cgtgctcccc ggagtggagg
      901 cggccgagcg cgccggggtg cccgccttcc tggagacctc cgcgccccgc aacctcccct
      961 tctacgagcg gctcggcttc accgtcaccg cc
//
