LOCUS       sequence-6844-ex        9614 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9614)
  TITLE     Unpublished, Paco
REFERENCE   2  (bases 1 to 9614)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"
COMMENT     Sequence Label: pYC6Lys-TRP1URA3
FEATURES             Location/Qualifiers
     source          1..9614
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        2..443
                     /gene="S. cerevisiae GAL1"
                     /label=GAL1 promoter
                     /note="inducible promoter, regulated by Gal4"
     protein_bind    2..119
                     /label=UAS
                     /bound_moiety="Gal4"
                     /note="upstream activating sequence mediating 
                     Gal4-dependent induction"
     primer_bind     414..437
                     /label=GAL1
                     /note="S. cerevisiae GAL1 promoter, forward primer"
     primer_bind     475..494
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        475..493
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             513..1184
                     /codon_start=1
                     /gene="S. cerevisiae TRP1"
                     /product="phosphoribosylanthranilate isomerase, required 
                     for tryptophan biosynthesis"
                     /label=TRP1
                     /note="yeast auxotrophic marker"
                     /translation="MSVINFTGSSGPLVKVCGLQSTEAAECALDSDADLLGIICVPNRK
                     RTIDPVIARKISSLVKAYKNSSGTPKYLVGVFRNQPKEDVLALVNDYGIDIVQLHGDES
                     WQEYQEFLGLPVIKRLVFPKDCNILLSAASQKPHSFIPLFDSEAGGTGELLDWNSISDW
                     VGRQESPESLHFMLAGGLTPENVGDALRLNGVIGVDVSGGVETNGVKDSNKIANFVKNA
                     KK"
     CDS             1209..2006
                     /codon_start=1
                     /gene="S. cerevisiae URA3"
                     /product="orotidine-5'-phosphate decarboxylase, required 
                     for uracil biosynthesis"
                     /label=URA3
                     /note="yeast auxotrophic marker, counterselectable with 
                     5-fluoroorotic acid (5-FOA)"
                     /translation="SKATYKERAATHPSPVAAKLFNIMHEKQTNLCASLDVRTTKELLE
                     LVEALGPKICLLKTHVDILTDFSMEGTVKPLKALSAKYNFLLFEDRKFADIGNTVKLQY
                     SAGVYRIAEWADITNAHGVVGPGIVSGLKQAAEEVTKEPRGLLMLAELSCKGSLSTGEY
                     TKGTVDIAKSDKDFVIGFIAQRDMGGRDEGYDWLIMTPGVGLDDKGDALGQQYRTVDDV
                     VSTGSDIIIVGRGLFAKGRDAKVEGERYRKAGWEAYLRRCGQQN"
     CDS             2022..2063
                     /codon_start=1
                     /product="epitope tag from simian virus 5"
                     /label=V5 tag
                     /translation="GKPIPNPLLGLDST"
     CDS             2073..2090
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     terminator      2111..2358
                     /gene="S. cerevisiae CYC1"
                     /label=CYC1 terminator
                     /note="transcription terminator for CYC1"
     primer_bind     complement(2127..2145)
                     /label=CYC1
                     /note="CYC1 transcription termination signal, reverse 
                     primer"
     primer_bind     complement(2435..2452)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(2606..3194)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(2686..2705)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(3365..4156,4157..4225))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKRSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     3988..4007
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     CDS             complement(4464..8642)
                     /codon_start=1
                     /gene="S. cerevisiae LYS2"
                     /product="alpha-aminoadipate reductase, required for lysine
                     biosynthesis"
                     /label=LYS2
                     /note="yeast auxotrophic marker"
                     /translation="MTNEKVWIEKLDNPTLSVLPHDFLRPQQEPYTKQATYSLQLPQLD
                     VPHDSFSNKYAVALSVWAALIYRVTGDDDIVLYIANNKILRFNIQPTWSFNELYSTINN
                     ELNKLNSIEANFSFDELAEKIQSCQDLERTPQLFRLAFLENQDFKLDEFKHHLVDFALN
                     LDTSNNAHVLNLIYNSLLYSNERVTIVADQFTQYLTAALSDPSNCITKISLITASSKDS
                     LPDPTKNLGWCDFVGCIHDIFQDNAEAFPERTCVVETPTLNSDKSRSFTYRDINRTSNI
                     VAHYLIKTGIKRGDVVMIYSSRGVDLMVCVMGVLKAGATFSVIDPAYPPARQTIYLGVA
                     KPRGLIVIRAAGQLDQLVEDYINDELEIVSRINSIAIQENGTIEGGKLDNGEDVLAPYD
                     HYKDTRTGVVVGPDSNPTLSFTSGSEGIPKGVLGRHFSLAYYFNWMSKRFNLTENDKFT
                     MLSGIAHDPIQRDMFTPLFLGAQLYVPTQDDIGTPGRLAEWMSKYGCTVTHLTPAMGQL
                     LTAQATTPFPKLHHAFFVGDILTKRDCLRLQTLAENCRIVNMYGTTETQRAVSYFEVKS
                     KNDDPNFLKKLKDVMPAGKGMLNVQLLVVNRNDRTQICGIGEIGEIYVRAGGLAEGYRG
                     LPELNKEKFVNNWFVEKDHWNYLDKDNGEPWRQFWLGPRDRLYRTGDLGRYLPNGDCEC
                     CGRADDQVKIRGFRIELGEIDTHISQHPLVRENITLVRKNADNEPTLITFMVPRFDKPD
                     DLSKFQSDVPKEVETDPIVKGLIGYHLLSKDIRTFLKKRLASYAMPSLIVVMDKLPLNP
                     NGKVDKPKLQFPTPKQLNLVAENTVSETDDSQFTNVEREVRDLWLSILPTKPASVSPDD
                     SFFDLGGHSILATKMIFTLKKKLQVDLPLGTIFKYPTIKAFAAEIDRIKSSGGSSQGEV
                     VENVTANYAEDAKKLVETLPSSYPSREYFVEPNSAEGKTTINVFVTGVTGFLGSYILAD
                     LLGRSPKNYSFKVFAHVRAKDEEAAFARLQKAGITYGTWNEKFASNIKVVLGDLSKSQF
                     GLSDEKWMDLANTVDIIIHNGALVHWVYPYAKLRDPNVISTINVMSLAAVGKPKFFDFV
                     SSTSTLDTEYYFNLSDKLVSEGKPGILESDDLMNSASGLTGGYGQSKWAAEYIIRRAGE
                     RGLRGCIVRPGYVTGASANGSSNTDDFLLRFLKGSVQLGKIPDIENSVNMVPVDHVARV
                     VVATSLNPPKENELAVAQVTGHPRILFKDYLYTLHDYGYDVEIESYSKWKKSLEASVID
                     RNEENALYPLLHMVLDNLPESTKAPELDDRNAVASLKKDTAWTGVDWSNGIGVTPEEVG
                     IYIAFLNKVGFLPPPTHNDKLPLPSIELTQAQISLVASGAGARGSSAAA"
     promoter        complement(8643..8882)
                     /gene="S. cerevisiae LYS2"
                     /label=LYS2 promoter
     misc_feature    8958..9461
                     /label=CEN/ARS
                     /note="S. cerevisiae CEN6 centromere fused to an 
                     autonomously replicating sequence"
     primer_bind     9491..9510
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
ORIGIN
        1 acggattaga agccgccgag cgggtgacag ccctccgaag gaagactctc ctccgtgcgt
       61 cctcgtcttc accggtcgcg ttcctgaaac gcagatgtgc ctcgcgccgc actgctccga
      121 acaataaaga ttctacaata ctagctttta tggttatgaa gaggaaaaat tggcagtaac
      181 ctggccccac aaaccttcaa atgaacgaat caaattaaca accataggat gataatgcga
      241 ttagtttttt agccttattt ctggggtaat taatcagcga agcgatgatt tttgatctat
      301 taacagatat ataaatgcaa aaactgcata accactttaa ctaatacttt caacattttc
      361 ggtttgtatt acttcttatt caaatgtaat aaaagtatca acaaaaaatt gttaatatac
      421 ctctatactt taacgtcaag gagaaaaaac cccggatcgg actactagca gctgtaatac
      481 gactcactat agggaatatt aagctcgccc ttatgtctgt tattaatttc acaggtagtt
      541 ctggtccatt ggtgaaagtt tgcggcttgc agagcacaga ggccgcagaa tgtgctctag
      601 attccgatgc tgacttgctg ggtattatat gtgtgcccaa tagaaagaga acaattgacc
      661 cggttattgc aaggaaaatt tcaagtcttg taaaagcata taaaaatagt tcaggcactc
      721 cgaaatactt ggttggcgtg tttcgtaatc aacctaagga ggatgttttg gctctggtca
      781 atgattacgg cattgatatc gtccaactgc atggagatga gtcgtggcaa gaataccaag
      841 agttcctcgg tttgccagtt attaaaagac tcgtatttcc aaaagactgc aacatactac
      901 tcagtgcagc ttcacagaaa cctcattcgt ttattccctt gtttgattca gaagcaggtg
      961 ggacaggtga acttttggat tggaactcga tttctgactg ggttggaagg caagagagcc
     1021 ccgaaagctt acattttatg ttagctggtg gactgacgcc agaaaatgtt ggtgatgcgc
     1081 ttagattaaa tggcgttatt ggtgttgatg taagcggagg tgtggagaca aatggtgtaa
     1141 aagactctaa caaaatagca aatttcgtca aaaatgctaa gaaaaagggc gagcttcgag
     1201 gtcacccatc gaaagctaca tataaggaac gtgctgctac tcatcctagt cctgttgctg
     1261 ccaagctatt taatatcatg cacgaaaagc aaacaaactt gtgtgcttca ttggatgttc
     1321 gtaccaccaa ggaattactg gagttagttg aagcattagg tcccaaaatt tgtttactaa
     1381 aaacacatgt ggatatcttg actgattttt ccatggaggg cacagttaag ccgctaaagg
     1441 cattatccgc caagtacaat tttttactct tcgaagacag aaaatttgct gacattggta
     1501 atacagtcaa attgcagtac tctgcgggtg tatacagaat agcagaatgg gcagacatta
     1561 cgaatgcaca cggtgtggtg ggcccaggta ttgttagcgg tttgaagcag gcggcagaag
     1621 aagtaacaaa ggaacctaga ggccttttga tgttagcaga attgtcatgc aagggctccc
     1681 tatctactgg agaatatact aagggtactg ttgacattgc gaagagcgac aaagattttg
     1741 ttatcggctt tattgctcaa agagacatgg gtggaagaga tgaaggttac gattggttga
     1801 ttatgacacc cggtgtgggt ttagatgaca agggagacgc attgggtcaa cagtatagaa
     1861 ccgtggatga tgtggtctct acaggatctg acattattat tgttggaaga ggactatttg
     1921 caaagggaag ggatgctaag gtagagggtg aacgttacag aaaagcaggc tgggaagcat
     1981 atttgagaag atgcggccag caaaacggtc acccattcga aggtaagcct atccctaacc
     2041 ctctcctcgg tctcgattct acgcgtaccg gtcatcatca ccatcaccat tgagtttcta
     2101 gagggccgca tcatgtaatt agttatgtca cgcttacatt cacgccctcc ccccacatcc
     2161 gctctaaccg aaaaggaagg agttagacaa cctgaagtct aggtccctat ttattttttt
     2221 atagttatgt tagtattaag aacgttattt atatttcaaa tttttctttt ttttctgtac
     2281 agacgcgtgt acgcatgtaa cattatactg aaaaccttgc ttgagaaggt tttgggacgc
     2341 tcgaaggctt taatttgcaa gctgcggccc tgcattaatg aatcggccaa cgcgcgggga
     2401 gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     2461 tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     2521 aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag cccaggaacc
     2581 gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     2641 aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     2701 ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     2761 tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     2821 tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     2881 ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     2941 tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     3001 ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     3061 tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     3121 aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     3181 aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     3241 aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     3301 ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     3361 acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     3421 ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagcgc ttaccatctg
     3481 gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     3541 taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     3601 tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     3661 gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     3721 cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     3781 aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     3841 cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     3901 tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     3961 gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga actttaaaag
     4021 tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     4081 gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     4141 ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     4201 cgacacggaa atgttgaata ctcatactct tcctttttca atgggtaata actgatataa
     4261 ttaaattgaa gctctaattt gtgagtttag tagacatgca gatctccctg atttacagtt
     4321 cttattcaat aactaatatt ttattctctt attatatatt attctcggag tttttaagtg
     4381 acatcacccg aaaagaagct aagtctttct cctaattcat atttaattat tgtacatgga
     4441 catatcatac gtaatgctca accttaagct gctgcggagc ttccacgagc accagcacct
     4501 gaagcaacta gacttatttg cgcttgagtt agttctatac ttggcagtgg aagtttgtca
     4561 ttatgagttg gtggaggtaa aaatccaacc ttgtttaaaa atgcaatata tataccaacc
     4621 tcttctggag taacacctat tccattagac caatcaacac ctgtccatgc ggtgtctttc
     4681 tttaaagatg ccacggcgtt cctatcgtct agttccggag ctttggtact ttcaggtaag
     4741 ttgtctaaga ccatgtgtag caaaggatac aacgcatttt cttcattcct gtcaataaca
     4801 gacgcctcca atgatttctt ccatttagaa tagctttcga tttcgacatc gtaaccataa
     4861 tcgtgtaaag tatacaagta gtctttgaat aatattcttg ggtgacccgt tacttgagca
     4921 acggccaatt cattttcttt gggaggattc aaagacgtag caacaacaac acgagcaaca
     4981 tgatctactg gaaccatatt cacggaattt tcgatatctg gaatcttacc taattggact
     5041 gaacctttca aaaatctcaa taagaaatca tctgtgtttg aagaaccatt ggcagaggca
     5101 cctgttacgt aacctggtct gacaatacac ccacgtaggc ccctttcacc tgcacgtcta
     5161 atgatgtact cagcagccca tttggactga ccatatccac cagtgagccc gcttgcagag
     5221 ttcattaaat cgtctgattc taaaatgcct ggcttccctt cgctaacaag tttatctgac
     5281 aaattaaagt agtattcagt gtcaagagta gaagtggagg aaacaaagtc aaagaacttt
     5341 ggcttgccga cggcggctaa gctcataaca ttgatagttg aaataacatt tggatccctc
     5401 aatttggcat atggataaac ccagtgaact aacgcaccat tatggataat tatatcaact
     5461 gtgtttgcca aatccatcca cttctcatct gaaagaccaa attggctttt agataaatcg
     5521 cctaatacaa ctttaatatt tgaggcaaat ttttcgttcc aagtaccata ggtgatacct
     5581 gccttttgta atcttgcaaa tgcagcttct tcatccttgg ccctgacgtg ggcaaacact
     5641 ttgaaactgt agttctttgg agaacgtcct aacaaatctg caaggatgta ggagcccaga
     5701 aatcctgtga caccggtaac aaacacatta attgttgttt ttccttcggc actattaggt
     5761 tcaacaaaat attctcgaga ggggtacgaa cttggtagcg tctcaaccaa tttcttggcg
     5821 tcttccgcat aatttgcagt gacattttcg acgacctcac cttgagatga tccacccgat
     5881 gatttaattc tgtcaatttc cgcggcaaag gcctttatcg ttggatactt gaaaattgtg
     5941 cccaatggta aatcaacttg cagctttttc tttaaggtaa aaatcatttt ggtagccaag
     6001 atagaatgac cacctaaatc gaaaaacgaa tcatctggtg atacagatgc tggcttggta
     6061 ggtaatatac ttaaccataa gtctctaacc tcgcgctcaa cattggtaaa ctgagagtcg
     6121 tcagtttcag aaactgtatt ttcagctacc aaatttaatt gcttgggagt tgggaattga
     6181 agtttaggct tatcaacttt accatttgga ttcaatggta gtttatccat aaccacaatc
     6241 aaggaaggca tagcatagct agccaatctt ttctttaaga aagtcctgat gtccttggat
     6301 aaaagatggt aaccgattaa gcccttaact atagggtcag tttcaacctc ctttggaaca
     6361 tcactttgga acttagacaa gtcatctggc ttgtcaaatc ttgggaccat aaatgtgatc
     6421 aatgttggct cattgtcggc atttttgcga actaaagtaa tgttttctct taccaatgga
     6481 tgttgggaaa tgtgcgtatc tatttctcct aattcgattc tgaacccacg aattttaact
     6541 tgatcatcag ccctaccgca acattcacag tcaccgtttg gtagataacg acctaaatca
     6601 cccgttctgt acaatctatc tcttggacct aaccagaatt gtctccaagg ttcaccatta
     6661 tccttatcca aataattcca gtgatctttt tcaacaaacc agttgttcac aaatttttct
     6721 ttattcaatt ctggtaatcc tctataacct tcggccaaac cacctgcacg aacataaatc
     6781 tcacctattt cgccaatacc acatatttga gtacgatcgt tcctgttaac aactagtagc
     6841 tgaacgttca acataccttt accagcaggc atgacatctt tcaatttttt caaaaagttt
     6901 ggatcgtcat tttttgattt aacttcgaaa taagaaactg cacgctgtgt ttcagtggta
     6961 ccgtacatat taacaatacg gcaattttct gccaaggttt gtaacctcag acaatcacgt
     7021 tttgttaaaa tgtcacccac aaagaacgca tgatgtaact tagggaatgg tgtagtagct
     7081 tgggcagtaa gtaattgacc catggcaggt gttaaatggg taactgtgca accatactta
     7141 ctcatccatt ccgctaaacg gcccggtgta ccaatatcat cttgagtagg gacatacaat
     7201 tgggcaccta aaaataatgg tgtaaacata tctctttgaa ttggatcatg tgcaataccg
     7261 ctcagcattg tgaatttatc attttctgtt aagttgaacc ttttggacat ccaattgaaa
     7321 taataagcca aggaaaaatg tctaccaaga acacccttag gaataccttc ggaaccagat
     7381 gtgaaagata gggttgggtt ggaatctggt ccaactacaa cacctgttct ggtgtctttg
     7441 tagtgatcat atggagccaa aacatcctcg ccattgtcca atttgccacc ttcaatggta
     7501 ccattttctt gaatagcgat ggaattgatt cttgaaacaa tctccaattc atcattgatg
     7561 taatcttcta ctagttgatc caattgtcca gcagctctaa taacaatcaa cccacgtggt
     7621 ttagcaacac ctaagtaaat ggtttgtctg gctgggggat atgcagggtc gataactgaa
     7681 aaggttgcgc cggctttcaa gacacccatc acacatacca tcaaatccac acccctagaa
     7741 gaatagatca tcactacatc acctcttttg atacctgttt taatcaaata atgggcaact
     7801 atgttagaag tgcggttgat gtcgcgataa gtgaaagaac gggacttgtc ggaatttagt
     7861 gttggagtct ccacaacaca ggttctctct gggaaggctt cagcattgtc ctggaaaatg
     7921 tcgtgaatac accccacgaa atcgcaccag cccaagttct tagttggatc aggtaaacta
     7981 tccttggatg atgcggtgat cagagagatt ttagttatgc aattggatgg atcgcttagc
     8041 gcagcagtca aatattgagt aaattggtcc gcaacaatgg ttactctttc attcgaatac
     8101 agtaagctgt tataaattaa gttcaaaaca tgcgcattat tactggtatc caaattcaaa
     8161 gcaaagtcca ctaaatgatg cttgaactcg tctaatttga aatcttggtt ttccaaaaag
     8221 gccaaacgga acaactgagg ggtcctttcc agatcttggc aactttgaat tttttcagct
     8281 agctcgtcaa aggaaaaatt ggcctcaata gaattgagct tgttcaactc attgttaatt
     8341 gtagaataca gctcattaaa tgaccacgtt ggttgaatat tgaatcttaa gattttgtta
     8401 ttcgcaatat aaagaacaat atcatcgtca ccggttactc tatatatcaa tgcagcccat
     8461 acactcaaag cgacagcgta tttgttagaa aaactatcat gaggcacatc gagctgaggt
     8521 agctgtaacg aatatgtagc ttgtttcgta taaggttctt gttgtgggcg taaaaagtca
     8581 tgtggtaaca ctgaaagagt tggattatcc aacttctcta tccagacctt ttcgttagtc
     8641 attagtgagt aactctgtga tatctctcta taattagcag tttttcactg aaattccagg
     8701 aaaggtaata aactcagatt tttttttata ctattggctg cttgttactt atatatcttg
     8761 aacttctccc agcgggtctt caaatacatt tgggcgatgt tcatgttcat taggcaggta
     8821 tttcgacatt gagtcacacg cgaaaaaccg ccggaatttt ttatgtaatt gcaagtggaa
     8881 ttccgctggc aaaactattg aagagttttc ctcgctaaaa ctgtgcgatg cctctagctt
     8941 atcgatgggt ccttttcatc acgtgctata aaaataatta taatttaaat tttttaatat
     9001 aaatatataa attaaaaata gaaagtaaaa aaagaaatta aagaaaaaat agtttttgtt
     9061 ttccgaagat gtaaaagact ctagggggat cgccaacaaa tactaccttt tatcttgctc
     9121 ttcctgctct caggtattaa tgccgaattg tttcatcttg tctgtgtaga agaccacaca
     9181 cgaaaatcct gtgattttac attttactta tcgttaatcg aatgtatatc tatttaatct
     9241 gcttttcttg tctaataaat atatatgtaa agtacgcttt ttgttgaaat tttttaaacc
     9301 tttgtttatt tttttttctt cattccgtaa ctcttctacc ttctttattt actttctaaa
     9361 atccaaatac aaaacataaa aataaataaa cacagagtaa attcccaaat tattccatca
     9421 ttaaaagata cgaggcgcgt gtaagttaca ggcaagcgat ccgtccgccg gcgaacgtgg
     9481 cgagaaagga agggaagaaa gcgaaaggag cgggggctag ggcggtggga agtgtagggg
     9541 tcacgctggg cgtaaccacc acacccgccg cgcttaatgg ggcgctacag ggcgcgtggg
     9601 gatgatccac tagt
//
