LOCUS       sequence-68456-e        7308 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP4 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7308)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7308)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP4-bio
FEATURES             Location/Qualifiers
     source          1..7308
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2688..2743
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2742..2761)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4979..4997)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5065..5169
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5170..5238,5239..6030)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5388..5407)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6201..6789
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6690..6709
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6943..6960
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7077..7098
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7113..7118,7119..7136,7137..7143)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7151..7167
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7156..7178
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7175..7191
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7175..7191
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcctaca acatcgtgcc cgagaacggc cggatgctga
     1381 acatgcggat cctgggcgaa gagaagccca acgtggacgg cgtgagcacc agcaacaccc
     1441 ctggcggcaa tgaggccagc agcgccagcc ctaatctggc cgacgccgcc gagaagaagg
     1501 acgagaaaga ggccagcgag cagggcgagg aaagccacaa gaaagagaac agccaggaaa
     1561 gcgccaacgg caaggacgac gtgaaagagg aaaagaaaac caacgagaag aaagatgacg
     1621 gcaagaccga caaggtgcag gaaaaggtcc tggaaaagag ccccaaagaa agccagatgg
     1681 tggacgacaa gaagaaaacc gaggccatcc ccaagaaagt ggtgcagccc agcagcagca
     1741 attctggcgg ccacgtgggc gaggaagagg accacaacga gggcgagggc gagcacgagg
     1801 aagaagagga acacgaagag gacgacgacg atgaggacga tgacacctac aacaaggacg
     1861 acctggaaga tgaggacctg tgcaagcaca acaacggcgg ctgcggcgac gataagctgt
     1921 gcgagtacgt gggcaaccgg cgcgtgaagt gcaagtgcaa agagggctac aagctggaag
     1981 gcatcgagtg cgtggagctg ctgtccctgg ccagcagctc tggcgcgccg tcgacctcca
     2041 tcacggccta taagagtgag ggggagtcag cggagttctc cttcccactc aaccttggag
     2101 aggaaagcct gcagggagag ttgagatgga aggcagagaa ggctccttct tcccagtcct
     2161 ggatcacctt ctccctaaag aaccaaaagg tgtctgtgca gaagtctact agcaacccca
     2221 agttccagct gtccgaaacg ctcccactca cccttcagat accccaggtc tcccttcagt
     2281 ttgctggttc tggcaacctg accctgactc tggacagagg gatactgtat caggaagtga
     2341 acctggtggt gatgaaagtg actcagcccg acagcaacac tttgacctgt gaggtgatgg
     2401 gacccacctc acccaagatg agactgatct tgaagcagga gaatcaggag gccagggtct
     2461 ccaggcagga gaaagtgatt caagtgcagg cccctgaagc aggggtgtgg caatgtctac
     2521 tgagtgaagg tgaagaggtc aagatggact ccaagatcca ggttttatcc aaagggttga
     2581 attccggatc actgcatcat attctggatg cacagaaaat ggtgtggaat catcgttaag
     2641 tctagacacg tgattaatta aggatccccc gacctcgacc tctggctaat aaaggaaatt
     2701 tattttcatt gcaatagtgt gttggaattt tttgtgtctc tcactcggaa ggacatatgg
     2761 gagggcaaat catttggtcg agatccctcg gagatctcta gctagaggat cgatccccgc
     2821 cccggacgaa ctaaacctga ctacgacatc tctgcccctt cttcgcgggg cagtgcatgt
     2881 aatcccttca gttggttggt acaacttgcc aactgggccc tgttccacat gtgacacggg
     2941 gggggaccaa acacaaaggg gttctctgac tgtagttgac atccttataa atggatgtgc
     3001 acatttgcca acactgagtg gctttcatcc tggagcagac tttgcagtct gtggactgca
     3061 acacaacatt gcctttatgt gtaactcttg gctgaagctc ttacaccaat gctgggggac
     3121 atgtacctcc caggggccca ggaagactac gggaggctac accaacgtca atcagagggg
     3181 cctgtgtagc taccgataag cggaccctca agagggcatt agcaatagtg tttataaggc
     3241 ccccttgtta accctaaacg ggtagcatat gcttcccggg tagtagtata tactatccag
     3301 actaacccta attcaatagc atatgttacc caacgggaag catatgctat cgaattaggg
     3361 ttagtaaaag ggtcctaagg aacagcgata tctcccaccc catgagctgt cacggtttta
     3421 tttacatggg gtcaggattc cacgagggta gtgaaccatt ttagtcacaa gggcagtggc
     3481 tgaagatcaa ggagcgggca gtgaactctc ctgaatcttc gcctgcttct tcattctcct
     3541 tcgtttagct aatagaataa ctgctgagtt gtgaacagta aggtgtatgt gaggtgctcg
     3601 aaaacaaggt ttcaggtgac gcccccagaa taaaatttgg acggggggtt cagtggtggc
     3661 attgtgctat gacaccaata taaccctcac aaaccccttg ggcaataaat actagtgtag
     3721 gaatgaaaca ttctgaatat ctttaacaat agaaatccat ggggtgggga caagccgtaa
     3781 agactggatg tccatctcac acgaatttat ggctatgggc aacacataat cctagtgcaa
     3841 tatgatactg gggttattaa gatgtgtccc aggcagggac caagacaggt gaaccatgtt
     3901 gttacactct atttgtaaca aggggaaaga gagtggacgc cgacagcagc ggactccact
     3961 ggttgtctct aacacccccg aaaattaaac ggggctccac gccaatgggg cccataaaca
     4021 aagacaagtg gccactcttt tttttgaaat tgtggagtgg gggcacgcgt cagcccccac
     4081 acgccgccct gcggttttgg actgtaaaat aagggtgtaa taacttggct gattgtaacc
     4141 ccgctaacca ctgcggtcaa accacttgcc cacaaaacca ctaatggcac cccggggaat
     4201 acctgcataa gtaggtgggc gggccaagat aggggcgcga ttgctgcgat ctggaggaca
     4261 aattacacac acttgcgcct gagcgccaag cacagggttg ttggtcctca tattcacgag
     4321 gtcgctgaga gcacggtggg ctaatgttgc catgggtagc atatactacc caaatatctg
     4381 gatagcatat gctatcctaa tctatatctg ggtagcatag gctatcctaa tctatatctg
     4441 ggtagcatat gctatcctaa tctatatctg ggtagtatat gctatcctaa tttatatctg
     4501 ggtagcatag gctatcctaa tctatatctg ggtagcatat gctatcctaa tctatatctg
     4561 ggtagtatat gctatcctaa tctgtatccg ggtagcatat gctatcctaa tagagattag
     4621 ggtagtatat gctatcctaa tttatatctg ggtagcatat actacccaaa tatctggata
     4681 gcatatgcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4741 gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta tatctgggta
     4801 gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta tatctgggta
     4861 gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg tatccgggta
     4921 gcatatgcta tcctcatgat aagctgtcaa acatgagaat tttcttgaag acgaaagggc
     4981 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
     5041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
     5101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
     5161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
     5221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
     5281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
     5341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
     5401 gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
     5461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
     5521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
     5581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
     5641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
     5701 accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
     5761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
     5821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
     5881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
     5941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
     6001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
     6061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
     6121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
     6181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
     6241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
     6301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
     6361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
     6421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
     6481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
     6541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
     6601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
     6661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
     6721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
     6781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
     6841 gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt
     6901 gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag
     6961 gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa
     7021 tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat
     7081 gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg
     7141 ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac
     7201 gccaagctct agctagaggt cgaccaattc tcatgtttga cagcttatca tcgcagatcc
     7261 gggcaacgtt gttgccattg ctgcaggcgc agaactggta ggtatgga
//
