LOCUS       sequence-68457-e        7323 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP5 ectodomain
            with no N-linked glycans upon cotransfection with BirA in mammalian 
            cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7323)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 7323)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP5-bio
FEATURES             Location/Qualifiers
     source          1..7323
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    2703..2758
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(2757..2776)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(4994..5012)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5080..5184
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5185..5253,5254..6045)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5403..5422)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6216..6804
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     6705..6724
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     6958..6975
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7092..7113
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7128..7133,7134..7151,7152..7158)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7166..7182
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7171..7193
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7190..7206
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7190..7206
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgccaaca acaagaacga gaacttcctg gtcgtgcggc
     1381 ggctgatgaa cgatgagaag ggcgagggcg gcttcaccag caagaacaaa gagaacggca
     1441 acaacaaccg gaacaacgag aatgagctga aagaggaagg cagcctgccc accaagatga
     1501 acgagaagaa cagcaacagc gccgacaagc agcccaacga catcagccac gacgagagca
     1561 agagcaactc caacaacgcc cagaacatcc agaaagagcc cgaggaaaaa gagaacagca
     1621 atcccaacct ggacagcagc gagaatagcg ccgagagcgc caccagaagc gtggacatca
     1681 gcgagcacaa tagcaacaat cctgagacaa aagaggaaaa cggcgaggaa ccactggacc
     1741 tggaaatcaa cgagaacgcc gagatcggcc aggaaccccc caaccggctg cacttcgaca
     1801 acgtggacga cgaggtgcca cactacagcg ccctgcggta caacaaggtg gagaagaacg
     1861 tggccgatga gatgctgctg tacaacatga tgagcgacca gaaccggaag tcctgcgcca
     1921 tcaacaacgg cggctgcagc gacgaccaga tctgcatcaa catcaacaac atcggcgtga
     1981 agtgcatctg caaggacggc tatctgctgg gcaccaagtg catcatcctg aactctggcg
     2041 cgccgtcgac ctccatcacg gcctataaga gtgaggggga gtcagcggag ttctccttcc
     2101 cactcaacct tggagaggaa agcctgcagg gagagttgag atggaaggca gagaaggctc
     2161 cttcttccca gtcctggatc accttctccc taaagaacca aaaggtgtct gtgcagaagt
     2221 ctactagcaa ccccaagttc cagctgtccg aaacgctccc actcaccctt cagatacccc
     2281 aggtctccct tcagtttgct ggttctggca acctgaccct gactctggac agagggatac
     2341 tgtatcagga agtgaacctg gtggtgatga aagtgactca gcccgacagc aacactttga
     2401 cctgtgaggt gatgggaccc acctcaccca agatgagact gatcttgaag caggagaatc
     2461 aggaggccag ggtctccagg caggagaaag tgattcaagt gcaggcccct gaagcagggg
     2521 tgtggcaatg tctactgagt gaaggtgaag aggtcaagat ggactccaag atccaggttt
     2581 tatccaaagg gttgaattcc ggatcactgc atcatattct ggatgcacag aaaatggtgt
     2641 ggaatcatcg ttaagtctag acacgtgatt aattaaggat cccccgacct cgacctctgg
     2701 ctaataaagg aaatttattt tcattgcaat agtgtgttgg aattttttgt gtctctcact
     2761 cggaaggaca tatgggaggg caaatcattt ggtcgagatc cctcggagat ctctagctag
     2821 aggatcgatc cccgccccgg acgaactaaa cctgactacg acatctctgc cccttcttcg
     2881 cggggcagtg catgtaatcc cttcagttgg ttggtacaac ttgccaactg ggccctgttc
     2941 cacatgtgac acgggggggg accaaacaca aaggggttct ctgactgtag ttgacatcct
     3001 tataaatgga tgtgcacatt tgccaacact gagtggcttt catcctggag cagactttgc
     3061 agtctgtgga ctgcaacaca acattgcctt tatgtgtaac tcttggctga agctcttaca
     3121 ccaatgctgg gggacatgta cctcccaggg gcccaggaag actacgggag gctacaccaa
     3181 cgtcaatcag aggggcctgt gtagctaccg ataagcggac cctcaagagg gcattagcaa
     3241 tagtgtttat aaggccccct tgttaaccct aaacgggtag catatgcttc ccgggtagta
     3301 gtatatacta tccagactaa ccctaattca atagcatatg ttacccaacg ggaagcatat
     3361 gctatcgaat tagggttagt aaaagggtcc taaggaacag cgatatctcc caccccatga
     3421 gctgtcacgg ttttatttac atggggtcag gattccacga gggtagtgaa ccattttagt
     3481 cacaagggca gtggctgaag atcaaggagc gggcagtgaa ctctcctgaa tcttcgcctg
     3541 cttcttcatt ctccttcgtt tagctaatag aataactgct gagttgtgaa cagtaaggtg
     3601 tatgtgaggt gctcgaaaac aaggtttcag gtgacgcccc cagaataaaa tttggacggg
     3661 gggttcagtg gtggcattgt gctatgacac caatataacc ctcacaaacc ccttgggcaa
     3721 taaatactag tgtaggaatg aaacattctg aatatcttta acaatagaaa tccatggggt
     3781 ggggacaagc cgtaaagact ggatgtccat ctcacacgaa tttatggcta tgggcaacac
     3841 ataatcctag tgcaatatga tactggggtt attaagatgt gtcccaggca gggaccaaga
     3901 caggtgaacc atgttgttac actctatttg taacaagggg aaagagagtg gacgccgaca
     3961 gcagcggact ccactggttg tctctaacac ccccgaaaat taaacggggc tccacgccaa
     4021 tggggcccat aaacaaagac aagtggccac tctttttttt gaaattgtgg agtgggggca
     4081 cgcgtcagcc cccacacgcc gccctgcggt tttggactgt aaaataaggg tgtaataact
     4141 tggctgattg taaccccgct aaccactgcg gtcaaaccac ttgcccacaa aaccactaat
     4201 ggcaccccgg ggaatacctg cataagtagg tgggcgggcc aagatagggg cgcgattgct
     4261 gcgatctgga ggacaaatta cacacacttg cgcctgagcg ccaagcacag ggttgttggt
     4321 cctcatattc acgaggtcgc tgagagcacg gtgggctaat gttgccatgg gtagcatata
     4381 ctacccaaat atctggatag catatgctat cctaatctat atctgggtag cataggctat
     4441 cctaatctat atctgggtag catatgctat cctaatctat atctgggtag tatatgctat
     4501 cctaatttat atctgggtag cataggctat cctaatctat atctgggtag catatgctat
     4561 cctaatctat atctgggtag tatatgctat cctaatctgt atccgggtag catatgctat
     4621 cctaatagag attagggtag tatatgctat cctaatttat atctgggtag catatactac
     4681 ccaaatatct ggatagcata tgctatccta atctatatct gggtagcata tgctatccta
     4741 atctatatct gggtagcata ggctatccta atctatatct gggtagcata tgctatccta
     4801 atctatatct gggtagtata tgctatccta atttatatct gggtagcata ggctatccta
     4861 atctatatct gggtagcata tgctatccta atctatatct gggtagtata tgctatccta
     4921 atctgtatcc gggtagcata tgctatcctc atgataagct gtcaaacatg agaattttct
     4981 tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg
     5041 gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta
     5101 tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt
     5161 caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc
     5221 ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa
     5281 gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt
     5341 aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt
     5401 ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact cggtcgccgc
     5461 atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg
     5521 gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg
     5581 gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac
     5641 atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca
     5701 aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg caaactatta
     5761 actggcgaac tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat
     5821 aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa
     5881 tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag
     5941 ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat
     6001 agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt
     6061 tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg
     6121 aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga
     6181 gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta
     6241 atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa
     6301 gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact
     6361 gttcttctag tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca
     6421 tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt
     6481 accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg
     6541 ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag
     6601 cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta
     6661 agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat
     6721 ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg
     6781 tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc
     6841 ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac
     6901 cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc
     6961 gagtcagtga gcgaggaagc ggaagagcgc ccaatacgca aaccgcctct ccccgcgcgt
     7021 tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     7081 cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     7141 cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     7201 tatgaccatg attacgccaa gctctagcta gaggtcgacc aattctcatg tttgacagct
     7261 tatcatcgca gatccgggca acgttgttgc cattgctgca ggcgcagaac tggtaggtat
     7321 gga
//
