LOCUS       sequence-68458-e        8064 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses enzymatically monobiotinylated full-length MSP10 
            ectodomain with no N-linked glycans upon cotransfection with BirA in
            mammalian cells. Contains a C-terminal rat Cd4 d3+4 tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8064)
  AUTHORS   Crosnier C, Wanaguru M, McDade B, Osier FH, Marsh K, Rayner JC, 
            Wright GJ
  TITLE     A library of functional recombinant cell surface and secreted 
            Plasmodium falciparum merozoite proteins.
  JOURNAL   Mol Cell Proteomics. 2013 Sep 16.
  PUBMED    24043421
REFERENCE   2  (bases 1 to 8064)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Mol Cell 
            Proteomics. 2013 Sep 16."
COMMENT     Sequence Label: MSP10-bio
FEATURES             Location/Qualifiers
     source          1..8064
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        154..533
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        534..737
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     688..708
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     734..758
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     primer_bind     1239..1258
                     /label=pMT2-F
                     /note="Synthetic intron, forward primer"
     regulatory      1281..1290
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     polyA_signal    3444..3499
                     /label=beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(3498..3517)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(5735..5753)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5821..5925
                     /gene="bla"
                     /label=AmpR promoter
     CDS             join(5926..5994,5995..6786)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(6144..6163)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6957..7545
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7446..7465
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     primer_bind     7699..7716
                     /label=L4440
                     /note="L4440 vector, forward primer"
     protein_bind    7833..7854
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(7869..7874,7875..7892,7893..7899)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7907..7923
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     7912..7934
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     7931..7947
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     7931..7947
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
ORIGIN
        1 agatctatac attgaatcaa tattggcaat tagccatatt agtcattggt tatatagcat
       61 aaatcaatat tggctattgg ccattgcata cgttgtatct atatcataat atgtacattt
      121 atattggctc atgtccaata tgaccgccat gttgacattg attattgact agttattaat
      181 agtaatcaat tacggggtca ttagttcata gcccatatat ggagttccgc gttacataac
      241 ttacggtaaa tggcccgcct ggctgaccgc ccaacgaccc ccgcccattg acgtcaataa
      301 tgacgtatgt tcccatagta acgccaatag ggactttcca ttgacgtcaa tgggtggagt
      361 atttacggta aactgcccac ttggcagtac atcaagtgta tcatatgcca agtccgcccc
      421 ctattgacgt caatgacggt aaatggcccg cctggcatta tgcccagtac atgaccttac
      481 gggactttcc tacttggcag tacatctacg tattagtcat cgctattacc atggtgatgc
      541 ggttttggca gtacaccaat gggcgtggat agcggtttga ctcacgggga tttccaagtc
      601 tccaccccat tgacgtcaat gggagtttgt tttggcacca aaatcaacgg gactttccaa
      661 aatgtcgtaa taaccccgcc ccgttgacgc aaatgggcgg taggcgtgta cggtgggagg
      721 tctatataag cagagctcgt ttagtgaacc gtcagatcct cactctcttc cgcatcgctg
      781 tctgcgaggg ccagctgttg ggctcgcggt tgaggacaaa ctcttcgcgg tctttccagt
      841 actcttggat cggaaacccg tcggcctccg aacggtactc cgccaccgag ggacctgagc
      901 gagtccgcat cgaccggatc ggaaaacctc tcgagaaagg cgtctaacca gtcacagtcg
      961 caaggtaggc tgagcaccgt ggcgggcggc agcgggtggc ggtcggggtt gtttctggcg
     1021 gaggtgctgc tgatgatgta attaaagtag gcggtcttga gacggcggat ggtcgaggtg
     1081 aggtgtggca ggcttgagat ccagctgttg gggtgagtac tccctctcaa aagcgggcat
     1141 tacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat tcacctggcc
     1201 cgatctggcc atacacttga gtgacaatga catccacttt gcctttctct ccacaggtgt
     1261 ccactcccag gtccaagttt gccaccatgg agtttcagac ccaggtactc atgtccctgc
     1321 tgctctgcat gtctggtgcg gccgcccacg tggacgacat caagaacacc gcccagaaga
     1381 agatcaccta cgacaagtac aacaagaaca aagaaaacat gaataacgag aagaacgaca
     1441 acaaggacaa caaggacaac atctacaacg acaacatcaa taacgataat atcaacaatg
     1501 acaacattaa caatgaggac gagtacaagt tcctgagcat gaagcactac aaggacagcc
     1561 tgtccaacaa gctgaacaat gagaacgacc acatgaacta cctgatccgg aagcggaagg
     1621 acaacaccca gggcagccag cacttcaacg agaatatcga gaataatgag aacgtggaga
     1681 acaacgagaa cattgagaac aatgaaaata atgaaaatat tgagaacatt gagaacaacg
     1741 aaaacaatga aaacaatgag aatatcgaaa ataacgaaaa taacgaaaat aacgagaaca
     1801 gcgccatcat gaacagcgag agctacaaca acatcatcaa cagcaacgag cacaacgagg
     1861 aacagatcaa gaagaaagaa gaggacctga tcgaggcctt cttccccttc atcctgaaga
     1921 agctggacaa cgaggccctg agcctggaca acaagtacga cgactactac aatctgccca
     1981 acgaccacaa tgacgcccac aaagagaact ccgccgacca caatctgctg ggctacaagc
     2041 tgggcaacaa cctgaagtcc tacctgatcg aagagaacga cgtgtcccag aagaaaaccg
     2101 acgatatcaa cgaggccgcc agcagcgaca gcgagaacat ccaggaaatc ctgagcaccg
     2161 acagcaatac cgcccacctg aaagagcgga agaaccagaa ggcccctccc ggcgagcaca
     2221 agcccgaagt gaagaacgcc ctgctgaaca gccaggtggc cagccctaag ggcgaggacg
     2281 agaagaagtc ccagccccag caccctctgg tcaacagcgg cgaccagctg cagcacccca
     2341 aagagatcga cgagaacgcc gagaagatcc ggcggaccct gctgaaagag agccgggaca
     2401 tcaaaaatac cgccgccatc atcgacgaga cagtgtacaa gttcgagcag ctgatcatga
     2461 agggccggta ctacgccacc gccgtgcgga acttcgtgat cttcaaagtg aactacatct
     2521 gcgagtacag caagtgcggc cccaacagcc ggtgctacat cgtggagaag gacaaagagc
     2581 agtgccgctg ccggcccaac tacattgtgg acatgagcgt gaactacttc aagtgcatcc
     2641 ccatgaagga catgaactgc gccaagaaca acggcggctg cgacgtgaat gccgagtgca
     2701 ccatcgtgga gggcgccgtg aagtgccagt gcagccacct gtacttcggc gacggcgtgt
     2761 tttgcgtgaa aaacagcggc gcgccgtcga cctccatcac ggcctataag agtgaggggg
     2821 agtcagcgga gttctccttc ccactcaacc ttggagagga aagcctgcag ggagagttga
     2881 gatggaaggc agagaaggct ccttcttccc agtcctggat caccttctcc ctaaagaacc
     2941 aaaaggtgtc tgtgcagaag tctactagca accccaagtt ccagctgtcc gaaacgctcc
     3001 cactcaccct tcagataccc caggtctccc ttcagtttgc tggttctggc aacctgaccc
     3061 tgactctgga cagagggata ctgtatcagg aagtgaacct ggtggtgatg aaagtgactc
     3121 agcccgacag caacactttg acctgtgagg tgatgggacc cacctcaccc aagatgagac
     3181 tgatcttgaa gcaggagaat caggaggcca gggtctccag gcaggagaaa gtgattcaag
     3241 tgcaggcccc tgaagcaggg gtgtggcaat gtctactgag tgaaggtgaa gaggtcaaga
     3301 tggactccaa gatccaggtt ttatccaaag ggttgaattc cggatcactg catcatattc
     3361 tggatgcaca gaaaatggtg tggaatcatc gttaagtcta gacacgtgat taattaagga
     3421 tcccccgacc tcgacctctg gctaataaag gaaatttatt ttcattgcaa tagtgtgttg
     3481 gaattttttg tgtctctcac tcggaaggac atatgggagg gcaaatcatt tggtcgagat
     3541 ccctcggaga tctctagcta gaggatcgat ccccgccccg gacgaactaa acctgactac
     3601 gacatctctg ccccttcttc gcggggcagt gcatgtaatc ccttcagttg gttggtacaa
     3661 cttgccaact gggccctgtt ccacatgtga cacggggggg gaccaaacac aaaggggttc
     3721 tctgactgta gttgacatcc ttataaatgg atgtgcacat ttgccaacac tgagtggctt
     3781 tcatcctgga gcagactttg cagtctgtgg actgcaacac aacattgcct ttatgtgtaa
     3841 ctcttggctg aagctcttac accaatgctg ggggacatgt acctcccagg ggcccaggaa
     3901 gactacggga ggctacacca acgtcaatca gaggggcctg tgtagctacc gataagcgga
     3961 ccctcaagag ggcattagca atagtgttta taaggccccc ttgttaaccc taaacgggta
     4021 gcatatgctt cccgggtagt agtatatact atccagacta accctaattc aatagcatat
     4081 gttacccaac gggaagcata tgctatcgaa ttagggttag taaaagggtc ctaaggaaca
     4141 gcgatatctc ccaccccatg agctgtcacg gttttattta catggggtca ggattccacg
     4201 agggtagtga accattttag tcacaagggc agtggctgaa gatcaaggag cgggcagtga
     4261 actctcctga atcttcgcct gcttcttcat tctccttcgt ttagctaata gaataactgc
     4321 tgagttgtga acagtaaggt gtatgtgagg tgctcgaaaa caaggtttca ggtgacgccc
     4381 ccagaataaa atttggacgg ggggttcagt ggtggcattg tgctatgaca ccaatataac
     4441 cctcacaaac cccttgggca ataaatacta gtgtaggaat gaaacattct gaatatcttt
     4501 aacaatagaa atccatgggg tggggacaag ccgtaaagac tggatgtcca tctcacacga
     4561 atttatggct atgggcaaca cataatccta gtgcaatatg atactggggt tattaagatg
     4621 tgtcccaggc agggaccaag acaggtgaac catgttgtta cactctattt gtaacaaggg
     4681 gaaagagagt ggacgccgac agcagcggac tccactggtt gtctctaaca cccccgaaaa
     4741 ttaaacgggg ctccacgcca atggggccca taaacaaaga caagtggcca ctcttttttt
     4801 tgaaattgtg gagtgggggc acgcgtcagc ccccacacgc cgccctgcgg ttttggactg
     4861 taaaataagg gtgtaataac ttggctgatt gtaaccccgc taaccactgc ggtcaaacca
     4921 cttgcccaca aaaccactaa tggcaccccg gggaatacct gcataagtag gtgggcgggc
     4981 caagataggg gcgcgattgc tgcgatctgg aggacaaatt acacacactt gcgcctgagc
     5041 gccaagcaca gggttgttgg tcctcatatt cacgaggtcg ctgagagcac ggtgggctaa
     5101 tgttgccatg ggtagcatat actacccaaa tatctggata gcatatgcta tcctaatcta
     5161 tatctgggta gcataggcta tcctaatcta tatctgggta gcatatgcta tcctaatcta
     5221 tatctgggta gtatatgcta tcctaattta tatctgggta gcataggcta tcctaatcta
     5281 tatctgggta gcatatgcta tcctaatcta tatctgggta gtatatgcta tcctaatctg
     5341 tatccgggta gcatatgcta tcctaataga gattagggta gtatatgcta tcctaattta
     5401 tatctgggta gcatatacta cccaaatatc tggatagcat atgctatcct aatctatatc
     5461 tgggtagcat atgctatcct aatctatatc tgggtagcat aggctatcct aatctatatc
     5521 tgggtagcat atgctatcct aatctatatc tgggtagtat atgctatcct aatttatatc
     5581 tgggtagcat aggctatcct aatctatatc tgggtagcat atgctatcct aatctatatc
     5641 tgggtagtat atgctatcct aatctgtatc cgggtagcat atgctatcct catgataagc
     5701 tgtcaaacat gagaattttc ttgaagacga aagggcctcg tgatacgcct atttttatag
     5761 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     5821 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     5881 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     5941 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     6001 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     6061 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     6121 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg
     6181 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     6241 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     6301 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     6361 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     6421 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca
     6481 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     6541 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     6601 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     6661 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     6721 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     6781 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     6841 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     6901 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     6961 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     7021 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     7081 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     7141 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     7201 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     7261 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     7321 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     7381 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     7441 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     7501 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     7561 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta
     7621 tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc
     7681 agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cccaatacgc
     7741 aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     7801 gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     7861 ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     7921 caatttcaca caggaaacag ctatgaccat gattacgcca agctctagct agaggtcgac
     7981 caattctcat gtttgacagc ttatcatcgc agatccgggc aacgttgttg ccattgctgc
     8041 aggcgcagaa ctggtaggta tgga
//
